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Targeting the hydrophilic regions of recombinant proteins by MS via in-solution buffer-free trypsin digestion

Betancourt, Lázaro H. LU ; Espinosa, Luis A. ; Ramos, Yassel ; Bequet-Romero, Mónica ; Rodríguez, Elías N. ; Sánchez, Aniel LU ; Marko-Varga, Gyorgy LU ; González, Luis J. and Besada, Vladimir (2019) In European Journal of Mass Spectrometry
Abstract

A desalting step using reversed phase chromatography is a common practice prior to mass spectrometry analysis of proteolytic digests in spite of the detrimental exclusion of the hydrophilic peptides. The detection of such peptides is also important for the complete coverage of protein sequences and the analysis of posttranslational modifications as inquired by regulatory agencies for the commercialization of biotechnological products. The procedure described here, named in-solution buffer-free digestion, simplifies the sample processing and circumvents the above-mentioned limitations by allowing the detection of tryptic hydrophilic peptides via direct ESI-MS analysis. Two DNA recombinant proteins such as HBcAg (hepatitis B core antigen)... (More)

A desalting step using reversed phase chromatography is a common practice prior to mass spectrometry analysis of proteolytic digests in spite of the detrimental exclusion of the hydrophilic peptides. The detection of such peptides is also important for the complete coverage of protein sequences and the analysis of posttranslational modifications as inquired by regulatory agencies for the commercialization of biotechnological products. The procedure described here, named in-solution buffer-free digestion, simplifies the sample processing and circumvents the above-mentioned limitations by allowing the detection of tryptic hydrophilic peptides via direct ESI-MS analysis. Two DNA recombinant proteins such as HBcAg (hepatitis B core antigen) and fusion VEGF (vascular endothelial growth factor) were analyzed with the proposed in-solution buffer-free digestion allowing the detection of extremely hydrophilic di-, tri- and tetra-peptides, C-terminal His-tail peptide, as well as disulfide-containing peptides. All these molecular species are hardly seen in mass spectrometric analysis using a standard digestion that includes a C18-desalting step. The procedure was also successfully tried on hydrophilic tetra- and hexa-peptides of Ribonuclease B carrying an N-glycosylation site occupied with “high-mannose” N-glycan chains. The in-solution buffer-free digestion constitutes a simple and straightforward approach to analyse the hydrophilic proteolytic peptides which are commonly elusive to the detection by conventional mass spectrometric analysis.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
epub
subject
keywords
buffer-free, digestion, HBcAg, Hydrophilic, mass spectrometry, peptides, RNase B, sequence coverage, vascular endothelial growth factor
in
European Journal of Mass Spectrometry
publisher
I M Publications
external identifiers
  • pmid:31822129
  • scopus:85077168675
ISSN
1469-0667
DOI
10.1177/1469066719893492
language
English
LU publication?
yes
id
1426bf8e-d7ce-4a60-b884-387419a01998
date added to LUP
2020-01-16 11:12:17
date last changed
2020-01-17 03:00:04
@article{1426bf8e-d7ce-4a60-b884-387419a01998,
  abstract     = {<p>A desalting step using reversed phase chromatography is a common practice prior to mass spectrometry analysis of proteolytic digests in spite of the detrimental exclusion of the hydrophilic peptides. The detection of such peptides is also important for the complete coverage of protein sequences and the analysis of posttranslational modifications as inquired by regulatory agencies for the commercialization of biotechnological products. The procedure described here, named in-solution buffer-free digestion, simplifies the sample processing and circumvents the above-mentioned limitations by allowing the detection of tryptic hydrophilic peptides via direct ESI-MS analysis. Two DNA recombinant proteins such as HBcAg (hepatitis B core antigen) and fusion VEGF (vascular endothelial growth factor) were analyzed with the proposed in-solution buffer-free digestion allowing the detection of extremely hydrophilic di-, tri- and tetra-peptides, C-terminal His-tail peptide, as well as disulfide-containing peptides. All these molecular species are hardly seen in mass spectrometric analysis using a standard digestion that includes a C18-desalting step. The procedure was also successfully tried on hydrophilic tetra- and hexa-peptides of Ribonuclease B carrying an N-glycosylation site occupied with “high-mannose” N-glycan chains. The in-solution buffer-free digestion constitutes a simple and straightforward approach to analyse the hydrophilic proteolytic peptides which are commonly elusive to the detection by conventional mass spectrometric analysis.</p>},
  author       = {Betancourt, Lázaro H. and Espinosa, Luis A. and Ramos, Yassel and Bequet-Romero, Mónica and Rodríguez, Elías N. and Sánchez, Aniel and Marko-Varga, Gyorgy and González, Luis J. and Besada, Vladimir},
  issn         = {1469-0667},
  language     = {eng},
  month        = {12},
  publisher    = {I M Publications},
  series       = {European Journal of Mass Spectrometry},
  title        = {Targeting the hydrophilic regions of recombinant proteins by MS via in-solution buffer-free trypsin digestion},
  url          = {http://dx.doi.org/10.1177/1469066719893492},
  doi          = {10.1177/1469066719893492},
  year         = {2019},
}