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Carbon isotope signature variability among cultured microalgae: Influence of species, nutrients and growth

Brutemark, Andreas; Lindehoff, Elin; Graneli, Edna and Granéli, Wilhelm LU (2009) In Journal of Experimental Marine Biology and Ecology 372(1-2). p.98-105
Abstract
In this study we have investigated whether the carbon isotopic signature differs between different groups and species of marine phytoplankton depending on growth phase, nutrient conditions and salinity. The 15 investigated algal species, representing the Bacillariophyceae, Chlorophyceae, Cryptophyceae, Cyanophyceae, Dinophyceae and Haptophyceae classes were grown in batch monocultures and analysed for delta C-13 in both exponential and stationary phase. For all the cultured species, delta C-13 signatures ranged from -23.5 parts per thousand (Imantonia sp.) to - 12.3 parts per thousand (Nodulania spumigena) in the exponential phase and from - 18.8 parts per thousand (Amphidinium carterae) to - 8.0 parts per thousand (Anabaena lemmermannii)... (More)
In this study we have investigated whether the carbon isotopic signature differs between different groups and species of marine phytoplankton depending on growth phase, nutrient conditions and salinity. The 15 investigated algal species, representing the Bacillariophyceae, Chlorophyceae, Cryptophyceae, Cyanophyceae, Dinophyceae and Haptophyceae classes were grown in batch monocultures and analysed for delta C-13 in both exponential and stationary phase. For all the cultured species, delta C-13 signatures ranged from -23.5 parts per thousand (Imantonia sp.) to - 12.3 parts per thousand (Nodulania spumigena) in the exponential phase and from - 18.8 parts per thousand (Amphidinium carterae) to - 8.0 parts per thousand (Anabaena lemmermannii) in the stationary phase. Three species (Dunaliella tertiolecta, Rhodomonas sp.. Heterocapsa triquetra) were also grown under nutrient sufficient and nitrogen or phosphorus deficient conditions. Nitrogen limitation resulted in a more negative delta C-13 signature, whereas no effect could be observed during phosphorus limitation compared to nutrient sufficient conditions. Growth of Prymnesium parvum in two different salinities resulted in a more negative delta C-13 signature in the 26 parts per thousand-media compared to growth in 7 parts per thousand-media. Our results show that the carbon isotopic signature of phytoplankton may be affected by salinity, differ among different phytoplankton species, between exponential and stationary phase, as well as between nutrient treatments. (C) 2009 Elsevier B.V. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Stationary phase, Nutrients, Isotope, Exponential phase, Culture, Algae, Carbon
in
Journal of Experimental Marine Biology and Ecology
volume
372
issue
1-2
pages
98 - 105
publisher
Elsevier
external identifiers
  • wos:000265552700013
  • scopus:62949166481
ISSN
0022-0981
DOI
10.1016/j.jembe.2009.02.013
language
English
LU publication?
yes
id
5def8522-b30e-4b0f-8189-ede2e2f8cb0e (old id 1427920)
date added to LUP
2009-06-25 14:28:40
date last changed
2017-01-01 05:59:34
@article{5def8522-b30e-4b0f-8189-ede2e2f8cb0e,
  abstract     = {In this study we have investigated whether the carbon isotopic signature differs between different groups and species of marine phytoplankton depending on growth phase, nutrient conditions and salinity. The 15 investigated algal species, representing the Bacillariophyceae, Chlorophyceae, Cryptophyceae, Cyanophyceae, Dinophyceae and Haptophyceae classes were grown in batch monocultures and analysed for delta C-13 in both exponential and stationary phase. For all the cultured species, delta C-13 signatures ranged from -23.5 parts per thousand (Imantonia sp.) to - 12.3 parts per thousand (Nodulania spumigena) in the exponential phase and from - 18.8 parts per thousand (Amphidinium carterae) to - 8.0 parts per thousand (Anabaena lemmermannii) in the stationary phase. Three species (Dunaliella tertiolecta, Rhodomonas sp.. Heterocapsa triquetra) were also grown under nutrient sufficient and nitrogen or phosphorus deficient conditions. Nitrogen limitation resulted in a more negative delta C-13 signature, whereas no effect could be observed during phosphorus limitation compared to nutrient sufficient conditions. Growth of Prymnesium parvum in two different salinities resulted in a more negative delta C-13 signature in the 26 parts per thousand-media compared to growth in 7 parts per thousand-media. Our results show that the carbon isotopic signature of phytoplankton may be affected by salinity, differ among different phytoplankton species, between exponential and stationary phase, as well as between nutrient treatments. (C) 2009 Elsevier B.V. All rights reserved.},
  author       = {Brutemark, Andreas and Lindehoff, Elin and Graneli, Edna and Granéli, Wilhelm},
  issn         = {0022-0981},
  keyword      = {Stationary phase,Nutrients,Isotope,Exponential phase,Culture,Algae,Carbon},
  language     = {eng},
  number       = {1-2},
  pages        = {98--105},
  publisher    = {Elsevier},
  series       = {Journal of Experimental Marine Biology and Ecology},
  title        = {Carbon isotope signature variability among cultured microalgae: Influence of species, nutrients and growth},
  url          = {http://dx.doi.org/10.1016/j.jembe.2009.02.013},
  volume       = {372},
  year         = {2009},
}