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Genetic diversity and differentiation of allopolyploid Dactylorhiza (Orchidaceae) with particular focus on the Dactylorhiza majalis ssp traunsteineri/lapponica complex

Olofsson, Sofie LU and Hedrén, Mikael LU (2009) In Biological Journal of the Linnean Society 97(1). p.52-67
Abstract
Genetic differentiation of Dactylorhiza majalis ssp. traunsteineri from the Alps, Scandinavia, and Britain was studied and compared with other allotetraploid members of the systematically challenging genus Dactylorhiza. One-hundred and eleven populations from altogether 18 taxa were analysed for eight polymorphic plastid markers and two size-variable fragments from the nuclear internal transcribed spacer (ITS) region. In total, 60 plastid haplotypes and six ITS alleles were found among the 737 individuals analysed. No clear differentiation between populations of ssp. traunsteineri from the three regions was revealed. However, ssp. traunsteineri was genetically differentiated from Dactylorhiza baumanniana, Dactylorhiza elata, and D. majalis... (More)
Genetic differentiation of Dactylorhiza majalis ssp. traunsteineri from the Alps, Scandinavia, and Britain was studied and compared with other allotetraploid members of the systematically challenging genus Dactylorhiza. One-hundred and eleven populations from altogether 18 taxa were analysed for eight polymorphic plastid markers and two size-variable fragments from the nuclear internal transcribed spacer (ITS) region. In total, 60 plastid haplotypes and six ITS alleles were found among the 737 individuals analysed. No clear differentiation between populations of ssp. traunsteineri from the three regions was revealed. However, ssp. traunsteineri was genetically differentiated from Dactylorhiza baumanniana, Dactylorhiza elata, and D. majalis ssp. sphagnicola, although the majority of allotetraploid taxa remained inseparable. Judging from the degree of concerted evolution in ITS, D. majalis ssp. alpestris may be regarded as a relatively old allotetraploid, whereas ssp. baltica and ssp. purpurella may be considerably younger. Based on plastid data, the Alp region had the highest genetic diversity followed by Scandinavia and Britain. The geographic distribution of haplotypes provided support for possible refugial areas around the Alps and for several independent immigration routes into Scandinavia after the last ice age. (C) 2009 The Linnean Society of London, Biological Journal of the Linnean Society, 2009, 97, 52-67. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
plastid DNA, phylogeography, ITS, narrow-leaved marsh-orchid, polyploid, evolution
in
Biological Journal of the Linnean Society
volume
97
issue
1
pages
52 - 67
publisher
Linnean Society of London
external identifiers
  • wos:000265406800005
  • scopus:65249116377
ISSN
0024-4066
DOI
10.1111/j.1095-8312.2008.01183.x
language
English
LU publication?
yes
id
4a0ba458-a955-4070-8045-c6d29f47fcf9 (old id 1428085)
date added to LUP
2009-06-25 11:57:35
date last changed
2017-01-01 04:20:14
@article{4a0ba458-a955-4070-8045-c6d29f47fcf9,
  abstract     = {Genetic differentiation of Dactylorhiza majalis ssp. traunsteineri from the Alps, Scandinavia, and Britain was studied and compared with other allotetraploid members of the systematically challenging genus Dactylorhiza. One-hundred and eleven populations from altogether 18 taxa were analysed for eight polymorphic plastid markers and two size-variable fragments from the nuclear internal transcribed spacer (ITS) region. In total, 60 plastid haplotypes and six ITS alleles were found among the 737 individuals analysed. No clear differentiation between populations of ssp. traunsteineri from the three regions was revealed. However, ssp. traunsteineri was genetically differentiated from Dactylorhiza baumanniana, Dactylorhiza elata, and D. majalis ssp. sphagnicola, although the majority of allotetraploid taxa remained inseparable. Judging from the degree of concerted evolution in ITS, D. majalis ssp. alpestris may be regarded as a relatively old allotetraploid, whereas ssp. baltica and ssp. purpurella may be considerably younger. Based on plastid data, the Alp region had the highest genetic diversity followed by Scandinavia and Britain. The geographic distribution of haplotypes provided support for possible refugial areas around the Alps and for several independent immigration routes into Scandinavia after the last ice age. (C) 2009 The Linnean Society of London, Biological Journal of the Linnean Society, 2009, 97, 52-67.},
  author       = {Olofsson, Sofie and Hedrén, Mikael},
  issn         = {0024-4066},
  keyword      = {plastid DNA,phylogeography,ITS,narrow-leaved marsh-orchid,polyploid,evolution},
  language     = {eng},
  number       = {1},
  pages        = {52--67},
  publisher    = {Linnean Society of London},
  series       = {Biological Journal of the Linnean Society},
  title        = {Genetic diversity and differentiation of allopolyploid Dactylorhiza (Orchidaceae) with particular focus on the Dactylorhiza majalis ssp traunsteineri/lapponica complex},
  url          = {http://dx.doi.org/10.1111/j.1095-8312.2008.01183.x},
  volume       = {97},
  year         = {2009},
}