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Molecular analysis of FRIGIDA, a major determinant of natural variation in Arabidopsis flowering time

Johanson, Urban LU ; West, Joanne; Lister, Clare; Michaels, Scott; Amasino, Richard and Dean, Caroline (2000) In Science 290(5490). p.344-347
Abstract
Vernalization, the acceleration of flowering by a long period of cold temperature, ensures that many plants overwinter vegetatively and flower in spring. In Arabidopsis, allelic variation at the FRIGIDA (FRI) locus is a major determinant of natural variation in flowering time. Dominant alleles of FRI confer late flowering, which is reversed to earliness by vernalization. We cloned FRI and analyzed the molecular basis of the allelic variation. Most of the early-flowering ecotypes analyzed carry FRI alleles containing one of two different deletions that disrupt the open reading frame. Loss-of-function mutations at FRI have thus provided the basis for the evolution of many early-flowering ecotypes.
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author
organization
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type
Contribution to journal
publication status
published
subject
keywords
Sequence Deletion, Genetic, Amino Acid Substitution, Alleles, Arabidopsis/*genetics/physiology, *Arabidopsis Proteins, Cloning, Molecular, Plant, *Genes, Genes, Recessive, *Genetic Variation, Mutation, Open Reading Frames, Plant Proteins/chemistry/*genetics/physiology, Polymorphism, Polymerase Chain Reaction, Temperature
in
Science
volume
290
issue
5490
pages
344 - 347
publisher
The American Association for the Advancement of Science
external identifiers
  • scopus:0034644643
ISSN
1095-9203
DOI
10.1126/science.290.5490.344
language
English
LU publication?
yes
id
7b6fe467-3e9a-4cee-8dcc-f61ff722eada (old id 1428949)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11030654
date added to LUP
2009-06-23 16:28:27
date last changed
2017-11-19 04:22:37
@article{7b6fe467-3e9a-4cee-8dcc-f61ff722eada,
  abstract     = {Vernalization, the acceleration of flowering by a long period of cold temperature, ensures that many plants overwinter vegetatively and flower in spring. In Arabidopsis, allelic variation at the FRIGIDA (FRI) locus is a major determinant of natural variation in flowering time. Dominant alleles of FRI confer late flowering, which is reversed to earliness by vernalization. We cloned FRI and analyzed the molecular basis of the allelic variation. Most of the early-flowering ecotypes analyzed carry FRI alleles containing one of two different deletions that disrupt the open reading frame. Loss-of-function mutations at FRI have thus provided the basis for the evolution of many early-flowering ecotypes.},
  author       = {Johanson, Urban and West, Joanne and Lister, Clare and Michaels, Scott and Amasino, Richard and Dean, Caroline},
  issn         = {1095-9203},
  keyword      = {Sequence Deletion,Genetic,Amino Acid Substitution,Alleles,Arabidopsis/*genetics/physiology,*Arabidopsis Proteins,Cloning,Molecular,Plant,*Genes,Genes,Recessive,*Genetic Variation,Mutation,Open Reading Frames,Plant Proteins/chemistry/*genetics/physiology,Polymorphism,Polymerase Chain Reaction,Temperature},
  language     = {eng},
  number       = {5490},
  pages        = {344--347},
  publisher    = {The American Association for the Advancement of Science},
  series       = {Science},
  title        = {Molecular analysis of FRIGIDA, a major determinant of natural variation in Arabidopsis flowering time},
  url          = {http://dx.doi.org/10.1126/science.290.5490.344},
  volume       = {290},
  year         = {2000},
}