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Exploitation of Engineered Light-Switchable Myosin XI for Nanotechnological Applications

Salhotra, Aseem ; Rahman, Mohammad A. ; Ruijgrok, Paul V. ; Meinecke, Christoph R. ; Ušaj, Marko ; Zemsky, Sasha ; Lindberg, Frida W. LU ; Surendiran, Pradheebha LU ; Lyttleton, Roman W. LU and Linke, Heiner LU orcid , et al. (2023) In ACS Nano 17(17). p.17233-17244
Abstract

For certain nanotechnological applications of the contractile proteins actin and myosin, e.g., in biosensing and network-based biocomputation, it would be desirable to temporarily switch on/off motile function in parts of nanostructured devices, e.g., for sorting or programming. Myosin XI motor constructs, engineered with a light-switchable domain for switching actin motility between high and low velocities (light-sensitive motors (LSMs) below), are promising in this regard. However, they were not designed for use in nanotechnology, where longevity of operation, long shelf life, and selectivity of function in specific regions of a nanofabricated network are important. Here, we tested if these criteria can be fulfilled using existing LSM... (More)

For certain nanotechnological applications of the contractile proteins actin and myosin, e.g., in biosensing and network-based biocomputation, it would be desirable to temporarily switch on/off motile function in parts of nanostructured devices, e.g., for sorting or programming. Myosin XI motor constructs, engineered with a light-switchable domain for switching actin motility between high and low velocities (light-sensitive motors (LSMs) below), are promising in this regard. However, they were not designed for use in nanotechnology, where longevity of operation, long shelf life, and selectivity of function in specific regions of a nanofabricated network are important. Here, we tested if these criteria can be fulfilled using existing LSM constructs or if additional developments will be required. We demonstrated extended shelf life as well as longevity of the actin-propelling function compared to those in previous studies. We also evaluated several approaches for selective immobilization with a maintained actin propelling function in dedicated nanochannels only. Whereas selectivity was feasible using certain nanopatterning combinations, the reproducibility was not satisfactory. In summary, the study demonstrates the feasibility of using engineered light-controlled myosin XI motors for myosin-driven actin transport in nanotechnological applications. Before use for, e.g., sorting or programming, additional work is however needed to achieve reproducibility of the nanofabrication and, further, optimize the motor properties.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
actin, engineered myosin XI, light-switchable motor, nanofabrication, spatiotemporal motility control, surface chemistry
in
ACS Nano
volume
17
issue
17
pages
12 pages
publisher
The American Chemical Society (ACS)
external identifiers
  • pmid:37639711
  • scopus:85171203426
ISSN
1936-0851
DOI
10.1021/acsnano.3c05137
language
English
LU publication?
yes
id
142b37e0-b2a5-431c-ae85-074f59ce940e
date added to LUP
2024-01-12 13:23:21
date last changed
2024-04-13 06:51:58
@article{142b37e0-b2a5-431c-ae85-074f59ce940e,
  abstract     = {{<p>For certain nanotechnological applications of the contractile proteins actin and myosin, e.g., in biosensing and network-based biocomputation, it would be desirable to temporarily switch on/off motile function in parts of nanostructured devices, e.g., for sorting or programming. Myosin XI motor constructs, engineered with a light-switchable domain for switching actin motility between high and low velocities (light-sensitive motors (LSMs) below), are promising in this regard. However, they were not designed for use in nanotechnology, where longevity of operation, long shelf life, and selectivity of function in specific regions of a nanofabricated network are important. Here, we tested if these criteria can be fulfilled using existing LSM constructs or if additional developments will be required. We demonstrated extended shelf life as well as longevity of the actin-propelling function compared to those in previous studies. We also evaluated several approaches for selective immobilization with a maintained actin propelling function in dedicated nanochannels only. Whereas selectivity was feasible using certain nanopatterning combinations, the reproducibility was not satisfactory. In summary, the study demonstrates the feasibility of using engineered light-controlled myosin XI motors for myosin-driven actin transport in nanotechnological applications. Before use for, e.g., sorting or programming, additional work is however needed to achieve reproducibility of the nanofabrication and, further, optimize the motor properties.</p>}},
  author       = {{Salhotra, Aseem and Rahman, Mohammad A. and Ruijgrok, Paul V. and Meinecke, Christoph R. and Ušaj, Marko and Zemsky, Sasha and Lindberg, Frida W. and Surendiran, Pradheebha and Lyttleton, Roman W. and Linke, Heiner and Korten, Till and Bryant, Zev and Månsson, Alf}},
  issn         = {{1936-0851}},
  keywords     = {{actin; engineered myosin XI; light-switchable motor; nanofabrication; spatiotemporal motility control; surface chemistry}},
  language     = {{eng}},
  month        = {{09}},
  number       = {{17}},
  pages        = {{17233--17244}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{ACS Nano}},
  title        = {{Exploitation of Engineered Light-Switchable Myosin XI for Nanotechnological Applications}},
  url          = {{http://dx.doi.org/10.1021/acsnano.3c05137}},
  doi          = {{10.1021/acsnano.3c05137}},
  volume       = {{17}},
  year         = {{2023}},
}