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New sequence-based data on the relative DNA contents of chromosomes in the normal male and female human diploid genomes for radiation molecular cytogenetics.

Repin, Mikhail V ; Golubev, Pavel LU and Repina, Ludmila A (2009) In Molecular Cytogenetics 2.
Abstract
ABSTRACT: BACKGROUND: The objective of this work is to obtain the correct relative DNA contents of chromosomes in the normal male and female human diploid genomes for the use at FISH analysis of radiation-induced chromosome aberrations. RESULTS: The relative DNA contents of chromosomes in the male and female human diploid genomes have been calculated from the publicly available international Human Genome Project data. New sequence-based data on the relative DNA contents of human chromosomes were compared with the data recommended by the International Atomic Energy Agency in 2001. The differences in the values of the relative DNA contents of chromosomes obtained by using different approaches for 15 human chromosomes, mainly for large... (More)
ABSTRACT: BACKGROUND: The objective of this work is to obtain the correct relative DNA contents of chromosomes in the normal male and female human diploid genomes for the use at FISH analysis of radiation-induced chromosome aberrations. RESULTS: The relative DNA contents of chromosomes in the male and female human diploid genomes have been calculated from the publicly available international Human Genome Project data. New sequence-based data on the relative DNA contents of human chromosomes were compared with the data recommended by the International Atomic Energy Agency in 2001. The differences in the values of the relative DNA contents of chromosomes obtained by using different approaches for 15 human chromosomes, mainly for large chromosomes, were below 2%. For the chromosomes 13, 17, 20 and 22 the differences were above 5%. CONCLUSION: New sequence-based data on the relative DNA contents of chromosomes in the normal male and female human diploid genomes were obtained. This approach, based on the genome sequence, can be recommended for the use in radiation molecular cytogenetics. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Molecular Cytogenetics
volume
2
article number
13
publisher
BioMed Central (BMC)
external identifiers
  • pmid:19500331
  • scopus:68149181017
  • pmid:19500331
ISSN
1755-8166
DOI
10.1186/1755-8166-2-13
language
English
LU publication?
yes
id
7091b5c9-fe4b-447d-a221-7b6694868c69 (old id 1434453)
date added to LUP
2016-04-01 14:43:53
date last changed
2021-05-06 02:22:38
@article{7091b5c9-fe4b-447d-a221-7b6694868c69,
  abstract     = {ABSTRACT: BACKGROUND: The objective of this work is to obtain the correct relative DNA contents of chromosomes in the normal male and female human diploid genomes for the use at FISH analysis of radiation-induced chromosome aberrations. RESULTS: The relative DNA contents of chromosomes in the male and female human diploid genomes have been calculated from the publicly available international Human Genome Project data. New sequence-based data on the relative DNA contents of human chromosomes were compared with the data recommended by the International Atomic Energy Agency in 2001. The differences in the values of the relative DNA contents of chromosomes obtained by using different approaches for 15 human chromosomes, mainly for large chromosomes, were below 2%. For the chromosomes 13, 17, 20 and 22 the differences were above 5%. CONCLUSION: New sequence-based data on the relative DNA contents of chromosomes in the normal male and female human diploid genomes were obtained. This approach, based on the genome sequence, can be recommended for the use in radiation molecular cytogenetics.},
  author       = {Repin, Mikhail V and Golubev, Pavel and Repina, Ludmila A},
  issn         = {1755-8166},
  language     = {eng},
  publisher    = {BioMed Central (BMC)},
  series       = {Molecular Cytogenetics},
  title        = {New sequence-based data on the relative DNA contents of chromosomes in the normal male and female human diploid genomes for radiation molecular cytogenetics.},
  url          = {http://dx.doi.org/10.1186/1755-8166-2-13},
  doi          = {10.1186/1755-8166-2-13},
  volume       = {2},
  year         = {2009},
}