Lipopolysaccharide induces cell death in cultured porcine myenteric neurons.
(2005) In Digestive Diseases and Sciences 50(9). p.1661-1668- Abstract
- Enteric bacteria execute, via lipopolysaccharide (LPS), a pathogenic role in intestinal inflammation. The effects of LPS on survival and neurotransmitter expression in cultured porcine myenteric neurons were investigated. Myenteric neurons were isolated and cultured for 6 days in medium, in LPS (100 ng/ml) with or without α-ketoglutarate or the nitric oxide synthase (NOS) inhibitor L-NAME, in α-ketoglutarate or in the NO donor SNAP. Neuronal survival and expression of vasoactive intestinal peptide (VIP) and NOS were evaluated by immunocytochemistry. Addition of LPS significantly decreased neuronal survival; only 40% survived, compared to controls run in parallel. The LPS-induced neurotoxic effect was not counteracted by the simultaneous... (More)
- Enteric bacteria execute, via lipopolysaccharide (LPS), a pathogenic role in intestinal inflammation. The effects of LPS on survival and neurotransmitter expression in cultured porcine myenteric neurons were investigated. Myenteric neurons were isolated and cultured for 6 days in medium, in LPS (100 ng/ml) with or without α-ketoglutarate or the nitric oxide synthase (NOS) inhibitor L-NAME, in α-ketoglutarate or in the NO donor SNAP. Neuronal survival and expression of vasoactive intestinal peptide (VIP) and NOS were evaluated by immunocytochemistry. Addition of LPS significantly decreased neuronal survival; only 40% survived, compared to controls run in parallel. The LPS-induced neurotoxic effect was not counteracted by the simultaneous presence of α-ketoglutarate or L-NAME. Either SNAP or α-ketoglutarate influenced neuronal survival. Culturing, particularly in the presence of LPS, markedly increased the proportion of VIP-immunoreactive neurons; NOS-immunoreactive neurons were unchanged. The reported LPS-induced neurotoxicity indicates loss of enteric neurons as a consequence of intestinal inflammation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/143918
- author
- Arciszewski, Marcin ; Pierzynowski, Stefan LU and Ekblad, Eva LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Myenteric Plexus: cytology, Animals, Cell Culture Techniques, Cell Death, Cell Survival, Female, Immunohistochemistry, Inflammation, Intestinal Diseases: etiology, Intestinal Diseases: immunology, Intestinal Diseases: microbiology, Lipopolysaccharides: pharmacology, Male, Myenteric Plexus: pathology, Neurons: drug effects, Neurons: physiology, Neurotransmitters: biosynthesis, Research Support, Non-U.S. Gov't, Swine
- in
- Digestive Diseases and Sciences
- volume
- 50
- issue
- 9
- pages
- 1661 - 1668
- publisher
- Springer
- external identifiers
-
- wos:000231312700015
- pmid:16133966
- scopus:23944452404
- pmid:16133966
- ISSN
- 1573-2568
- DOI
- 10.1007/s10620-005-2912-2
- language
- English
- LU publication?
- yes
- id
- fa83cbee-9c50-43a1-92d6-e36db1bada1d (old id 143918)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16133966&dopt=Abstract
- date added to LUP
- 2016-04-01 11:59:10
- date last changed
- 2022-01-26 21:11:40
@article{fa83cbee-9c50-43a1-92d6-e36db1bada1d, abstract = {{Enteric bacteria execute, via lipopolysaccharide (LPS), a pathogenic role in intestinal inflammation. The effects of LPS on survival and neurotransmitter expression in cultured porcine myenteric neurons were investigated. Myenteric neurons were isolated and cultured for 6 days in medium, in LPS (100 ng/ml) with or without α-ketoglutarate or the nitric oxide synthase (NOS) inhibitor L-NAME, in α-ketoglutarate or in the NO donor SNAP. Neuronal survival and expression of vasoactive intestinal peptide (VIP) and NOS were evaluated by immunocytochemistry. Addition of LPS significantly decreased neuronal survival; only 40% survived, compared to controls run in parallel. The LPS-induced neurotoxic effect was not counteracted by the simultaneous presence of α-ketoglutarate or L-NAME. Either SNAP or α-ketoglutarate influenced neuronal survival. Culturing, particularly in the presence of LPS, markedly increased the proportion of VIP-immunoreactive neurons; NOS-immunoreactive neurons were unchanged. The reported LPS-induced neurotoxicity indicates loss of enteric neurons as a consequence of intestinal inflammation.}}, author = {{Arciszewski, Marcin and Pierzynowski, Stefan and Ekblad, Eva}}, issn = {{1573-2568}}, keywords = {{Myenteric Plexus: cytology; Animals; Cell Culture Techniques; Cell Death; Cell Survival; Female; Immunohistochemistry; Inflammation; Intestinal Diseases: etiology; Intestinal Diseases: immunology; Intestinal Diseases: microbiology; Lipopolysaccharides: pharmacology; Male; Myenteric Plexus: pathology; Neurons: drug effects; Neurons: physiology; Neurotransmitters: biosynthesis; Research Support; Non-U.S. Gov't; Swine}}, language = {{eng}}, number = {{9}}, pages = {{1661--1668}}, publisher = {{Springer}}, series = {{Digestive Diseases and Sciences}}, title = {{Lipopolysaccharide induces cell death in cultured porcine myenteric neurons.}}, url = {{https://lup.lub.lu.se/search/files/2731467/624981.pdf}}, doi = {{10.1007/s10620-005-2912-2}}, volume = {{50}}, year = {{2005}}, }