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Versican V2 Assembles the Extracellular Matrix Surrounding the Nodes of Ranvier in the CNS

Dours-Zimmermann, Maria T.; Maurer, Konrad; Rauch, Uwe LU ; Stoffel, Wilhelm; Faessler, Reinhard and Zimmermann, Dieter R. (2009) In Journal of Neuroscience 29(24). p.7731-7742
Abstract
The CNS-restricted versican splice-variant V2 is a large chondroitin sulfate proteoglycan incorporated in the extracellular matrix surrounding myelinated fibers and particularly accumulating at nodes of Ranvier. In vitro, it is a potent inhibitor of axonal growth and therefore considered to participate in the reduction of structural plasticity connected to myelination. To study the role of versican V2 during postnatal development, we designed a novel isoform-specific gene inactivation approach circumventing early embryonic lethality of the complete knock-out and preventing compensation by the remaining versican splice variants. These mice are viable and fertile; however, they display major molecular alterations at the nodes of Ranvier.... (More)
The CNS-restricted versican splice-variant V2 is a large chondroitin sulfate proteoglycan incorporated in the extracellular matrix surrounding myelinated fibers and particularly accumulating at nodes of Ranvier. In vitro, it is a potent inhibitor of axonal growth and therefore considered to participate in the reduction of structural plasticity connected to myelination. To study the role of versican V2 during postnatal development, we designed a novel isoform-specific gene inactivation approach circumventing early embryonic lethality of the complete knock-out and preventing compensation by the remaining versican splice variants. These mice are viable and fertile; however, they display major molecular alterations at the nodes of Ranvier. While the clustering of nodal sodium channels and paranodal structures appear in versican V2-deficient mice unaffected, the formation of the extracellular matrix surrounding the nodes is largely impaired. The conjoint loss of tenascin-R and phosphacan from the perinodal matrix provide strong evidence that versican V2, possibly controlled by a nodal receptor, organizes the extracellular matrix assembly in vivo. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Neuroscience
volume
29
issue
24
pages
7731 - 7742
publisher
Society for Neuroscience
external identifiers
  • wos:000267131000013
  • scopus:67449158777
ISSN
1529-2401
DOI
10.1523/JNEUROSCI.4158-08.2009
language
English
LU publication?
yes
id
2bed7299-ff8e-4e5a-9eee-74e89c7fbcbf (old id 1441285)
date added to LUP
2009-07-28 09:30:27
date last changed
2017-10-01 04:01:38
@article{2bed7299-ff8e-4e5a-9eee-74e89c7fbcbf,
  abstract     = {The CNS-restricted versican splice-variant V2 is a large chondroitin sulfate proteoglycan incorporated in the extracellular matrix surrounding myelinated fibers and particularly accumulating at nodes of Ranvier. In vitro, it is a potent inhibitor of axonal growth and therefore considered to participate in the reduction of structural plasticity connected to myelination. To study the role of versican V2 during postnatal development, we designed a novel isoform-specific gene inactivation approach circumventing early embryonic lethality of the complete knock-out and preventing compensation by the remaining versican splice variants. These mice are viable and fertile; however, they display major molecular alterations at the nodes of Ranvier. While the clustering of nodal sodium channels and paranodal structures appear in versican V2-deficient mice unaffected, the formation of the extracellular matrix surrounding the nodes is largely impaired. The conjoint loss of tenascin-R and phosphacan from the perinodal matrix provide strong evidence that versican V2, possibly controlled by a nodal receptor, organizes the extracellular matrix assembly in vivo.},
  author       = {Dours-Zimmermann, Maria T. and Maurer, Konrad and Rauch, Uwe and Stoffel, Wilhelm and Faessler, Reinhard and Zimmermann, Dieter R.},
  issn         = {1529-2401},
  language     = {eng},
  number       = {24},
  pages        = {7731--7742},
  publisher    = {Society for Neuroscience},
  series       = {Journal of Neuroscience},
  title        = {Versican V2 Assembles the Extracellular Matrix Surrounding the Nodes of Ranvier in the CNS},
  url          = {http://dx.doi.org/10.1523/JNEUROSCI.4158-08.2009},
  volume       = {29},
  year         = {2009},
}