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Seasonal changes in lipid composition and glycogen storage associated with freeze-tolerance of the earthworm, Dendrobaena octaedra

Overgaard, Johannes; Tollarova, Michaela; Hedlund, Katarina LU ; Petersen, Soren O. and Holmstrup, Martin (2009) In Journal of Comparative Physiology B 179(5). p.569-577
Abstract
The earthworm, Dendrobaena octaedra, is a common species in the uppermost soil and humus layers of coniferous forests and tundra in temperate and subarctic regions. The species is freeze-tolerant and may survive several months in a frozen state. Upon freezing, glycogen reserves are rapidly converted to glucose serving as a cryoprotectant and fuel for metabolism. In the present study we investigated the induction of freeze-tolerance under field conditions, and sought to find relationships between temperature, glycogen and fat reserves, membrane phospholipid composition and the degree of freeze-tolerance. Freeze-tolerance was induced when worms had experienced temperatures below 5A degrees C for 2 weeks or more. Freeze-tolerance was linked... (More)
The earthworm, Dendrobaena octaedra, is a common species in the uppermost soil and humus layers of coniferous forests and tundra in temperate and subarctic regions. The species is freeze-tolerant and may survive several months in a frozen state. Upon freezing, glycogen reserves are rapidly converted to glucose serving as a cryoprotectant and fuel for metabolism. In the present study we investigated the induction of freeze-tolerance under field conditions, and sought to find relationships between temperature, glycogen and fat reserves, membrane phospholipid composition and the degree of freeze-tolerance. Freeze-tolerance was induced when worms had experienced temperatures below 5A degrees C for 2 weeks or more. Freeze-tolerance was linked to the magnitude of glycogen reserves, which also fluctuated with field temperatures being highest in autumn and winter. On the other hand fat reserves seemed not to be linked with freeze-tolerance at all. However, high glycogen alone did not confer freeze-tolerance; alterations in the membrane phospholipid fatty acid composition (PLFA) were also necessary in order to secure freeze-tolerance. The changes in PLFA composition were generally similar to changes occurring in other ectothermic animals during winter acclimation with an increased degree of unsaturation of the PLFAs. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Winter, temperatures, Membrane lipids, Freeze-tolerance, Glycogen, Earthworms
in
Journal of Comparative Physiology B
volume
179
issue
5
pages
569 - 577
publisher
Springer
external identifiers
  • wos:000266948300003
  • scopus:67849108178
ISSN
0174-1578
DOI
10.1007/s00360-009-0341-9
language
English
LU publication?
yes
id
60fa8228-b919-417a-99d1-d46c4bb27925 (old id 1442042)
date added to LUP
2009-07-27 13:30:03
date last changed
2017-07-09 03:53:41
@article{60fa8228-b919-417a-99d1-d46c4bb27925,
  abstract     = {The earthworm, Dendrobaena octaedra, is a common species in the uppermost soil and humus layers of coniferous forests and tundra in temperate and subarctic regions. The species is freeze-tolerant and may survive several months in a frozen state. Upon freezing, glycogen reserves are rapidly converted to glucose serving as a cryoprotectant and fuel for metabolism. In the present study we investigated the induction of freeze-tolerance under field conditions, and sought to find relationships between temperature, glycogen and fat reserves, membrane phospholipid composition and the degree of freeze-tolerance. Freeze-tolerance was induced when worms had experienced temperatures below 5A degrees C for 2 weeks or more. Freeze-tolerance was linked to the magnitude of glycogen reserves, which also fluctuated with field temperatures being highest in autumn and winter. On the other hand fat reserves seemed not to be linked with freeze-tolerance at all. However, high glycogen alone did not confer freeze-tolerance; alterations in the membrane phospholipid fatty acid composition (PLFA) were also necessary in order to secure freeze-tolerance. The changes in PLFA composition were generally similar to changes occurring in other ectothermic animals during winter acclimation with an increased degree of unsaturation of the PLFAs.},
  author       = {Overgaard, Johannes and Tollarova, Michaela and Hedlund, Katarina and Petersen, Soren O. and Holmstrup, Martin},
  issn         = {0174-1578},
  keyword      = {Winter,temperatures,Membrane lipids,Freeze-tolerance,Glycogen,Earthworms},
  language     = {eng},
  number       = {5},
  pages        = {569--577},
  publisher    = {Springer},
  series       = {Journal of Comparative Physiology B},
  title        = {Seasonal changes in lipid composition and glycogen storage associated with freeze-tolerance of the earthworm, Dendrobaena octaedra},
  url          = {http://dx.doi.org/10.1007/s00360-009-0341-9},
  volume       = {179},
  year         = {2009},
}