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Refractometry of microscopic objects with digital holography

Gustafsson, Mats LU and Sebesta, Mikael LU (2004) In Applied Optics 43(25). p.4796-4801
Abstract
Digital holography has some desirable properties for refractometry of microscopic objects since it gives phase and amplitude information of an object in all depths of focus from one set of exposures. We show that the amplitude part of the image can be used to observe how the Becke lines move between different depths of focus and hence determine whether an object has a higher or a lower index of refraction than its surrounding medium, i.e., the sign of the relief. It is also shown that one single-phase image provides an independent technique to determine the sign of relief between an object and the surrounding medium.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Optics
volume
43
issue
25
pages
4796 - 4801
publisher
OSA
external identifiers
  • wos:000223663300003
  • pmid:15449465
  • scopus:4544340790
ISSN
2155-3165
DOI
10.1364/AO.43.004796
language
English
LU publication?
yes
id
940dbaa6-4cd1-472a-a7d2-9b3b421ae375 (old id 144341)
date added to LUP
2007-07-05 09:41:44
date last changed
2017-06-11 03:30:37
@article{940dbaa6-4cd1-472a-a7d2-9b3b421ae375,
  abstract     = {Digital holography has some desirable properties for refractometry of microscopic objects since it gives phase and amplitude information of an object in all depths of focus from one set of exposures. We show that the amplitude part of the image can be used to observe how the Becke lines move between different depths of focus and hence determine whether an object has a higher or a lower index of refraction than its surrounding medium, i.e., the sign of the relief. It is also shown that one single-phase image provides an independent technique to determine the sign of relief between an object and the surrounding medium.},
  author       = {Gustafsson, Mats and Sebesta, Mikael},
  issn         = {2155-3165},
  language     = {eng},
  number       = {25},
  pages        = {4796--4801},
  publisher    = {OSA},
  series       = {Applied Optics},
  title        = {Refractometry of microscopic objects with digital holography},
  url          = {http://dx.doi.org/10.1364/AO.43.004796},
  volume       = {43},
  year         = {2004},
}