Refractometry of microscopic objects with digital holography
Gustafsson, Mats LU
and Sebesta, Mikael
LU
(2004)
In Applied Optics
43(25).
p.4796-4801
- Abstract
- Digital holography has some desirable properties for refractometry of microscopic objects since it gives phase and amplitude information of an object in all depths of focus from one set of exposures. We show that the amplitude part of the image can be used to observe how the Becke lines move between different depths of focus and hence determine whether an object has a higher or a lower index of refraction than its surrounding medium, i.e., the sign of the relief. It is also shown that one single-phase image provides an independent technique to determine the sign of relief between an object and the surrounding medium.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/144341
- author
- Gustafsson, Mats
LU
and Sebesta, Mikael
LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Applied Optics
- volume
- 43
- issue
- 25
- pages
- 4796 - 4801
- publisher
- Optical Society of America
- external identifiers
-
- wos:000223663300003
- pmid:15449465
- scopus:4544340790
- ISSN
- 2155-3165
- DOI
- 10.1364/AO.43.004796
- language
- English
- LU publication?
- yes
- id
- 940dbaa6-4cd1-472a-a7d2-9b3b421ae375 (old id 144341)
- date added to LUP
- 2016-04-01 11:51:10
- date last changed
- 2022-01-26 19:14:44
@article{940dbaa6-4cd1-472a-a7d2-9b3b421ae375,
abstract = {{Digital holography has some desirable properties for refractometry of microscopic objects since it gives phase and amplitude information of an object in all depths of focus from one set of exposures. We show that the amplitude part of the image can be used to observe how the Becke lines move between different depths of focus and hence determine whether an object has a higher or a lower index of refraction than its surrounding medium, i.e., the sign of the relief. It is also shown that one single-phase image provides an independent technique to determine the sign of relief between an object and the surrounding medium.}},
author = {{Gustafsson, Mats and Sebesta, Mikael}},
issn = {{2155-3165}},
language = {{eng}},
number = {{25}},
pages = {{4796--4801}},
publisher = {{Optical Society of America}},
series = {{Applied Optics}},
title = {{Refractometry of microscopic objects with digital holography}},
url = {{http://dx.doi.org/10.1364/AO.43.004796}},
doi = {{10.1364/AO.43.004796}},
volume = {{43}},
year = {{2004}},
}