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Stromal cell-derived inducing activity does not promote dopaminergic differentiation, but enhances differentiation and proliferation of neural stem cell-derived astrocytes.

Roybon, Laurent LU ; Brundin, Patrik LU and Li, Jia-Yi LU (2005) In Experimental Neurology 196(2). p.373-380
Abstract
Previous evidence has shown that stromal cell-derived inducing activity (SDIA), produced by the mouse PA6 stromal cell line, promotes dopaminergic differentiation of mouse, monkey and human embryonic stem cells in vitro. To examine whether PA6 stromal cells can enhance the yield of dopaminergic differentiation from neural progenitors, we generated neurospheres from embryonic day 11.5 (E11.5) (midbrain and forebrain) and E14.5 (ventral mesencephalon and cortex) rat embryos and allowed them to differentiate in co-culture with PA6 cells or poly-L-lysine/laminin-coated dishes. We observed that SDIA did not promote dopaminergic differentiation of E11.5 and E14.5 neurospheres but more prominently, enhanced astrocyte differentiation, cell... (More)
Previous evidence has shown that stromal cell-derived inducing activity (SDIA), produced by the mouse PA6 stromal cell line, promotes dopaminergic differentiation of mouse, monkey and human embryonic stem cells in vitro. To examine whether PA6 stromal cells can enhance the yield of dopaminergic differentiation from neural progenitors, we generated neurospheres from embryonic day 11.5 (E11.5) (midbrain and forebrain) and E14.5 (ventral mesencephalon and cortex) rat embryos and allowed them to differentiate in co-culture with PA6 cells or poly-L-lysine/laminin-coated dishes. We observed that SDIA did not promote dopaminergic differentiation of E11.5 and E14.5 neurospheres but more prominently, enhanced astrocyte differentiation, cell survival and astrocyte proliferation. Our results suggest that PA6 cells do not have a general capacity to promote differentiation into dopaminergic neurons from all types of stem cells, but that they may specifically induce dopaminergic differentiation of highly uncommitted stem cells such as embryonic stem cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
astrocytes, dopaminergic neurons, neurosphere, PA6 stromal cell, neural stem cells
in
Experimental Neurology
volume
196
issue
2
pages
373 - 380
publisher
Academic Press
external identifiers
  • wos:000233547700018
  • pmid:16199035
  • scopus:27744478490
ISSN
0014-4886
DOI
10.1016/j.expneurol.2005.08.023
language
English
LU publication?
yes
id
782ab644-07a1-48b3-8c0a-087f1d9e6be0 (old id 144922)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16199035&dopt=Abstract
date added to LUP
2007-07-19 10:37:41
date last changed
2017-04-30 10:00:48
@article{782ab644-07a1-48b3-8c0a-087f1d9e6be0,
  abstract     = {Previous evidence has shown that stromal cell-derived inducing activity (SDIA), produced by the mouse PA6 stromal cell line, promotes dopaminergic differentiation of mouse, monkey and human embryonic stem cells in vitro. To examine whether PA6 stromal cells can enhance the yield of dopaminergic differentiation from neural progenitors, we generated neurospheres from embryonic day 11.5 (E11.5) (midbrain and forebrain) and E14.5 (ventral mesencephalon and cortex) rat embryos and allowed them to differentiate in co-culture with PA6 cells or poly-L-lysine/laminin-coated dishes. We observed that SDIA did not promote dopaminergic differentiation of E11.5 and E14.5 neurospheres but more prominently, enhanced astrocyte differentiation, cell survival and astrocyte proliferation. Our results suggest that PA6 cells do not have a general capacity to promote differentiation into dopaminergic neurons from all types of stem cells, but that they may specifically induce dopaminergic differentiation of highly uncommitted stem cells such as embryonic stem cells.},
  author       = {Roybon, Laurent and Brundin, Patrik and Li, Jia-Yi},
  issn         = {0014-4886},
  keyword      = {astrocytes,dopaminergic neurons,neurosphere,PA6 stromal cell,neural stem cells},
  language     = {eng},
  number       = {2},
  pages        = {373--380},
  publisher    = {Academic Press},
  series       = {Experimental Neurology},
  title        = {Stromal cell-derived inducing activity does not promote dopaminergic differentiation, but enhances differentiation and proliferation of neural stem cell-derived astrocytes.},
  url          = {http://dx.doi.org/10.1016/j.expneurol.2005.08.023},
  volume       = {196},
  year         = {2005},
}