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Myosin 5a controls insulin granule recruitment during late-phase secretion.

Ivarsson, Rosita LU ; Jing, Xingjun LU ; Waselle, Laurent; Regazzi, Romano and Renström, Erik LU (2005) In Traffic: the International Journal of Intracellular Transport 6(11). p.1027-1035
Abstract
We have examined the importance of the actin-based molecular motor myosin 5a for insulin granule transport and insulin secretion. Expression of myosin 5a was downregulated in clonal INS-1E cells using RNAinterference. Stimulated hormone secretion was reduced by 46% and single-cell exocytosis, measured by capacitance recordings, was inhibited by 42% after silencing. Silencing of Slac-2c/MYRIP, which links insulin granules to myosin 5a, resulted in similar inhibition of single-cell exocytosis. Antibody inhibition of the myosin 5a-Slac-2c/MYRIP interaction significantly reduced the recruitment of insulin granules for release. The pool of releasable granules independent of myosin 5a activity was estimated to approximately 550 granules. Total... (More)
We have examined the importance of the actin-based molecular motor myosin 5a for insulin granule transport and insulin secretion. Expression of myosin 5a was downregulated in clonal INS-1E cells using RNAinterference. Stimulated hormone secretion was reduced by 46% and single-cell exocytosis, measured by capacitance recordings, was inhibited by 42% after silencing. Silencing of Slac-2c/MYRIP, which links insulin granules to myosin 5a, resulted in similar inhibition of single-cell exocytosis. Antibody inhibition of the myosin 5a-Slac-2c/MYRIP interaction significantly reduced the recruitment of insulin granules for release. The pool of releasable granules independent of myosin 5a activity was estimated to approximately 550 granules. Total internal reflection microscopy was then applied to directly investigate granule recruitment to the plasma membrane. Silencing of myosin 5a inhibited granule recruitment during late phase of insulin secretion. In conclusion, we propose a model where insulin granules are transported through the actin network via both myosin 5a-mediated transport and via passive diffusion, with the former playing the major role during stimulatory conditions. (Less)
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Contribution to journal
publication status
published
subject
in
Traffic: the International Journal of Intracellular Transport
volume
6
issue
11
pages
1027 - 1035
publisher
Wiley-Blackwell
external identifiers
  • pmid:16190983
  • wos:000232169600007
  • scopus:26944465715
ISSN
1398-9219
DOI
10.1111/j.1600-0854.2005.00342.x
language
English
LU publication?
yes
id
1ac3541f-b9ec-494d-bfa5-23e31ac45179 (old id 144988)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16190983&dopt=Abstract
date added to LUP
2007-07-13 14:20:23
date last changed
2017-08-13 03:38:08
@article{1ac3541f-b9ec-494d-bfa5-23e31ac45179,
  abstract     = {We have examined the importance of the actin-based molecular motor myosin 5a for insulin granule transport and insulin secretion. Expression of myosin 5a was downregulated in clonal INS-1E cells using RNAinterference. Stimulated hormone secretion was reduced by 46% and single-cell exocytosis, measured by capacitance recordings, was inhibited by 42% after silencing. Silencing of Slac-2c/MYRIP, which links insulin granules to myosin 5a, resulted in similar inhibition of single-cell exocytosis. Antibody inhibition of the myosin 5a-Slac-2c/MYRIP interaction significantly reduced the recruitment of insulin granules for release. The pool of releasable granules independent of myosin 5a activity was estimated to approximately 550 granules. Total internal reflection microscopy was then applied to directly investigate granule recruitment to the plasma membrane. Silencing of myosin 5a inhibited granule recruitment during late phase of insulin secretion. In conclusion, we propose a model where insulin granules are transported through the actin network via both myosin 5a-mediated transport and via passive diffusion, with the former playing the major role during stimulatory conditions.},
  author       = {Ivarsson, Rosita and Jing, Xingjun and Waselle, Laurent and Regazzi, Romano and Renström, Erik},
  issn         = {1398-9219},
  language     = {eng},
  number       = {11},
  pages        = {1027--1035},
  publisher    = {Wiley-Blackwell},
  series       = {Traffic: the International Journal of Intracellular Transport},
  title        = {Myosin 5a controls insulin granule recruitment during late-phase secretion.},
  url          = {http://dx.doi.org/10.1111/j.1600-0854.2005.00342.x},
  volume       = {6},
  year         = {2005},
}