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Acoustic Whole Blood Plasmapheresis Chip for Prostate Specific Antigen Microarray Diagnostics.

Lenshof, Andreas LU ; Ahmad Tajudin, Asilah LU ; Järås, Kerstin LU ; Swärd-Nilsson, Ann-Margret; Åberg, Lena; Marko-Varga, György LU ; Malm, Johan LU ; Lilja, Hans LU and Laurell, Thomas LU (2009) In Analytical Chemistry 81(Jul 13). p.6030-6037
Abstract
The generation of high quality plasma from whole blood is of major interest for many biomedical analyses and clinical diagnostic methods. However, it has proven to be a major challenge to make use of microfluidic separation devices to process fluids with high cell content, such as whole blood. Here, we report on an acoustophoresis based separation chip that prepares diagnostic plasma from whole blood linked to a clinical application. This acoustic separator has the capacity to sequentially remove enriched blood cells in multiple steps to yield high quality plasma of low cellular content. The generated plasma fulfills the standard requirements (<6.0 x 10(9) erythrocytes/L) recommended by the Council of Europe. Further, we successfully... (More)
The generation of high quality plasma from whole blood is of major interest for many biomedical analyses and clinical diagnostic methods. However, it has proven to be a major challenge to make use of microfluidic separation devices to process fluids with high cell content, such as whole blood. Here, we report on an acoustophoresis based separation chip that prepares diagnostic plasma from whole blood linked to a clinical application. This acoustic separator has the capacity to sequentially remove enriched blood cells in multiple steps to yield high quality plasma of low cellular content. The generated plasma fulfills the standard requirements (<6.0 x 10(9) erythrocytes/L) recommended by the Council of Europe. Further, we successfully linked the plasmapheresis microchip to our previously developed porous silicon sandwich antibody microarray chip for prostate specific antigen (PSA) detection. PSA was detected by good linearity (R(2) > 0.99) in the generated plasma via fluorescence readout without any signal amplification at clinically relevant levels (0.19-21.8 ng/mL). (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
81
issue
Jul 13
pages
6030 - 6037
publisher
The American Chemical Society
external identifiers
  • wos:000268455600012
  • pmid:19594154
  • scopus:68049113566
ISSN
1520-6882
DOI
10.1021/ac9013572
language
English
LU publication?
yes
id
9fb466b2-e376-428c-8e99-5e9f5311d968 (old id 1453134)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19594154?dopt=Abstract
date added to LUP
2009-08-04 11:37:16
date last changed
2017-12-17 04:03:25
@article{9fb466b2-e376-428c-8e99-5e9f5311d968,
  abstract     = {The generation of high quality plasma from whole blood is of major interest for many biomedical analyses and clinical diagnostic methods. However, it has proven to be a major challenge to make use of microfluidic separation devices to process fluids with high cell content, such as whole blood. Here, we report on an acoustophoresis based separation chip that prepares diagnostic plasma from whole blood linked to a clinical application. This acoustic separator has the capacity to sequentially remove enriched blood cells in multiple steps to yield high quality plasma of low cellular content. The generated plasma fulfills the standard requirements (&lt;6.0 x 10(9) erythrocytes/L) recommended by the Council of Europe. Further, we successfully linked the plasmapheresis microchip to our previously developed porous silicon sandwich antibody microarray chip for prostate specific antigen (PSA) detection. PSA was detected by good linearity (R(2) &gt; 0.99) in the generated plasma via fluorescence readout without any signal amplification at clinically relevant levels (0.19-21.8 ng/mL).},
  author       = {Lenshof, Andreas and Ahmad Tajudin, Asilah and Järås, Kerstin and Swärd-Nilsson, Ann-Margret and Åberg, Lena and Marko-Varga, György and Malm, Johan and Lilja, Hans and Laurell, Thomas},
  issn         = {1520-6882},
  language     = {eng},
  number       = {Jul 13},
  pages        = {6030--6037},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Acoustic Whole Blood Plasmapheresis Chip for Prostate Specific Antigen Microarray Diagnostics.},
  url          = {http://dx.doi.org/10.1021/ac9013572},
  volume       = {81},
  year         = {2009},
}