Advanced

Acoustic differential extraction for forensic analysis of sexual assault evidence.

Norris, Jessica Voorhees; Evander, Mikael LU ; Horsman-Hall, Katie M; Nilsson, Johan LU ; Laurell, Thomas LU and Landers, James P (2009) In Analytical Chemistry 81(15). p.6089-6095
Abstract
Forensic DNA analysis of samples obtained from sexual assault evidence relies on separation of male and female components of the recovered genetic material. The conventional separation method used by crime laboratories, differential extraction (DE), is one of the most time-consuming sample preparation steps, requires extensive sample handling, is difficult to automate, and often results in inefficient separation of female DNA from the male sample components. To circumvent conventional DE, acoustic differential extraction (ADE) analysis was developed on a microfluidic device. The ADE method relies on acoustic trapping of sperm cells in the presence of epithelial cell lysate (which is unretained), and laminar flow valving to direct the male... (More)
Forensic DNA analysis of samples obtained from sexual assault evidence relies on separation of male and female components of the recovered genetic material. The conventional separation method used by crime laboratories, differential extraction (DE), is one of the most time-consuming sample preparation steps, requires extensive sample handling, is difficult to automate, and often results in inefficient separation of female DNA from the male sample components. To circumvent conventional DE, acoustic differential extraction (ADE) analysis was developed on a microfluidic device. The ADE method relies on acoustic trapping of sperm cells in the presence of epithelial cell lysate (which is unretained), and laminar flow valving to direct the male and female fractions to separate outlets. Following the separation of sperm from epithelial cell lysate, DNA extraction, quantitation, amplification, and separation were performed using conventional laboratory methods. The results show that highly purified male and female fractions can be obtained with the ADE microdevice from mock sexual assault samples in 14 min. ADE analysis provides the potential to significantly alter the means by which sexual assault evidence is processed in crime laboratories. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
81
issue
15
pages
6089 - 6095
publisher
The American Chemical Society
external identifiers
  • wos:000268455600019
  • pmid:19591449
  • scopus:68049094085
ISSN
1520-6882
DOI
10.1021/ac900439b
language
English
LU publication?
yes
id
d3971fee-4704-442f-914c-f8fd8447336e (old id 1453177)
date added to LUP
2009-09-01 08:48:10
date last changed
2017-11-12 03:20:26
@article{d3971fee-4704-442f-914c-f8fd8447336e,
  abstract     = {Forensic DNA analysis of samples obtained from sexual assault evidence relies on separation of male and female components of the recovered genetic material. The conventional separation method used by crime laboratories, differential extraction (DE), is one of the most time-consuming sample preparation steps, requires extensive sample handling, is difficult to automate, and often results in inefficient separation of female DNA from the male sample components. To circumvent conventional DE, acoustic differential extraction (ADE) analysis was developed on a microfluidic device. The ADE method relies on acoustic trapping of sperm cells in the presence of epithelial cell lysate (which is unretained), and laminar flow valving to direct the male and female fractions to separate outlets. Following the separation of sperm from epithelial cell lysate, DNA extraction, quantitation, amplification, and separation were performed using conventional laboratory methods. The results show that highly purified male and female fractions can be obtained with the ADE microdevice from mock sexual assault samples in 14 min. ADE analysis provides the potential to significantly alter the means by which sexual assault evidence is processed in crime laboratories.},
  author       = {Norris, Jessica Voorhees and Evander, Mikael and Horsman-Hall, Katie M and Nilsson, Johan and Laurell, Thomas and Landers, James P},
  issn         = {1520-6882},
  language     = {eng},
  number       = {15},
  pages        = {6089--6095},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Acoustic differential extraction for forensic analysis of sexual assault evidence.},
  url          = {http://dx.doi.org/10.1021/ac900439b},
  volume       = {81},
  year         = {2009},
}