Is there a specific lysophospholipase in human pancreatic juice?
(1993) In Biochimica et Biophysica Acta 1167(3). p.326-330- Abstract
- The existence of a specific lysophospholipase in human pancreatic juice was evaluated. The proteins were separated by a series of chromatographic steps including Sephacryl S-200, cholate-Sepharose 4B, Sephadex G-100 and CM-Sephadex G-50. The enzyme activities against 1-palmitoyl lysolecithin (LL) as well as tributyrin (TB) and p-nitrophenyl butyrate (PNPB) were determined in all the fractions of these purification procedures. Enzyme activity against LL was always eluted in parallel with activities against TB and PNPB, and no unique activity against LL could be found. The specific activity against LL was 40-times lower than that against PNPB and 200-times lower than that against TB. It is concluded that there is no unique lysophospholipase... (More)
- The existence of a specific lysophospholipase in human pancreatic juice was evaluated. The proteins were separated by a series of chromatographic steps including Sephacryl S-200, cholate-Sepharose 4B, Sephadex G-100 and CM-Sephadex G-50. The enzyme activities against 1-palmitoyl lysolecithin (LL) as well as tributyrin (TB) and p-nitrophenyl butyrate (PNPB) were determined in all the fractions of these purification procedures. Enzyme activity against LL was always eluted in parallel with activities against TB and PNPB, and no unique activity against LL could be found. The specific activity against LL was 40-times lower than that against PNPB and 200-times lower than that against TB. It is concluded that there is no unique lysophospholipase in human pancreatic juice and that the hydrolysis of lysolecithin is most likely performed by carboxyl ester lipase. (Less)
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https://lup.lub.lu.se/record/1454023c-8c86-4140-a875-32c2f9b48831
- author
- Duan, Rui-Dong LU and Borgström, Bengt
- organization
- publishing date
- 1993
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biochimica et Biophysica Acta
- volume
- 1167
- issue
- 3
- pages
- 5 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0027154721
- ISSN
- 0006-3002
- DOI
- 10.1016/0005-2760(93)90236-3
- language
- English
- LU publication?
- yes
- id
- 1454023c-8c86-4140-a875-32c2f9b48831
- date added to LUP
- 2019-02-03 09:49:51
- date last changed
- 2024-01-15 13:44:28
@article{1454023c-8c86-4140-a875-32c2f9b48831, abstract = {{The existence of a specific lysophospholipase in human pancreatic juice was evaluated. The proteins were separated by a series of chromatographic steps including Sephacryl S-200, cholate-Sepharose 4B, Sephadex G-100 and CM-Sephadex G-50. The enzyme activities against 1-palmitoyl lysolecithin (LL) as well as tributyrin (TB) and p-nitrophenyl butyrate (PNPB) were determined in all the fractions of these purification procedures. Enzyme activity against LL was always eluted in parallel with activities against TB and PNPB, and no unique activity against LL could be found. The specific activity against LL was 40-times lower than that against PNPB and 200-times lower than that against TB. It is concluded that there is no unique lysophospholipase in human pancreatic juice and that the hydrolysis of lysolecithin is most likely performed by carboxyl ester lipase.}}, author = {{Duan, Rui-Dong and Borgström, Bengt}}, issn = {{0006-3002}}, language = {{eng}}, number = {{3}}, pages = {{326--330}}, publisher = {{Elsevier}}, series = {{Biochimica et Biophysica Acta}}, title = {{Is there a specific lysophospholipase in human pancreatic juice?}}, url = {{http://dx.doi.org/10.1016/0005-2760(93)90236-3}}, doi = {{10.1016/0005-2760(93)90236-3}}, volume = {{1167}}, year = {{1993}}, }