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Association between major histocompatibility complex class IIB alleles and resistance to Aeromonas salmonicida in Atlantic salmon

Langefors, Åsa LU ; Lohm, Jakob LU ; Grahn, Mats LU ; Andersen, O and von Schantz, Torbjörn LU (2001) In Royal Society of London. Proceedings B. Biological Sciences 268(1466). p.479-485
Abstract
We have tested the importance of genetic variation in the major histocompatibility complex (MHC) class IIB in Atlantic salmon (Salmo salar) for survival after challenge with a highly virulent bacterial pathogen. Forty juvenile full siblings from each of 120 families were infected with the bacterium Aeromonas salmonicida, which causes high mortality in salmon due to furunculosis. Fishes from high-resistance (HR, < 35% mortality) and low-resistance (LR, > 80% mortality) families were screened for their MHC class IIB genotypes using the denaturing gradient gel electrophoresis (DGGE) technique. The exon 2 sequences, encoding the major part of the peptide-binding region, were established far each DGGE fragment. One allele, e, containing a... (More)
We have tested the importance of genetic variation in the major histocompatibility complex (MHC) class IIB in Atlantic salmon (Salmo salar) for survival after challenge with a highly virulent bacterial pathogen. Forty juvenile full siblings from each of 120 families were infected with the bacterium Aeromonas salmonicida, which causes high mortality in salmon due to furunculosis. Fishes from high-resistance (HR, < 35% mortality) and low-resistance (LR, > 80% mortality) families were screened for their MHC class IIB genotypes using the denaturing gradient gel electrophoresis (DGGE) technique. The exon 2 sequences, encoding the major part of the peptide-binding region, were established far each DGGE fragment. One allele, e, containing a missense single base substitution was significantly more prevalent in HR families than in LR families. An odds-ratio test showed that broods carrying this allele had a 12-fold higher chance of being HR than broods without the e allele. A second allele, i, showed significantly higher frequencies in uninfected and surviving individuals than in infected dead individuals. A third allele, j, tended to be more prevalent both in LR families and in individuals that had died of the infection. There was no correlation between MHC heterozygosity and resistance to A. salmonicida. Our results support the hypothesis that MHC polymorphism is maintained through pathogen-driven selection acting by means of frequency-dependent selection rather than heterozygous advantage. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Royal Society of London. Proceedings B. Biological Sciences
volume
268
issue
1466
pages
479 - 485
publisher
Royal Society
external identifiers
  • scopus:0035819811
ISSN
1471-2954
DOI
10.1098/rspb.2000.1378
language
English
LU publication?
yes
id
39467973-10f9-45db-9a8b-fc5dd2244766 (old id 145856)
date added to LUP
2007-06-26 09:07:27
date last changed
2018-06-24 03:42:19
@article{39467973-10f9-45db-9a8b-fc5dd2244766,
  abstract     = {We have tested the importance of genetic variation in the major histocompatibility complex (MHC) class IIB in Atlantic salmon (Salmo salar) for survival after challenge with a highly virulent bacterial pathogen. Forty juvenile full siblings from each of 120 families were infected with the bacterium Aeromonas salmonicida, which causes high mortality in salmon due to furunculosis. Fishes from high-resistance (HR, &lt; 35% mortality) and low-resistance (LR, &gt; 80% mortality) families were screened for their MHC class IIB genotypes using the denaturing gradient gel electrophoresis (DGGE) technique. The exon 2 sequences, encoding the major part of the peptide-binding region, were established far each DGGE fragment. One allele, e, containing a missense single base substitution was significantly more prevalent in HR families than in LR families. An odds-ratio test showed that broods carrying this allele had a 12-fold higher chance of being HR than broods without the e allele. A second allele, i, showed significantly higher frequencies in uninfected and surviving individuals than in infected dead individuals. A third allele, j, tended to be more prevalent both in LR families and in individuals that had died of the infection. There was no correlation between MHC heterozygosity and resistance to A. salmonicida. Our results support the hypothesis that MHC polymorphism is maintained through pathogen-driven selection acting by means of frequency-dependent selection rather than heterozygous advantage.},
  author       = {Langefors, Åsa and Lohm, Jakob and Grahn, Mats and Andersen, O and von Schantz, Torbjörn},
  issn         = {1471-2954},
  language     = {eng},
  number       = {1466},
  pages        = {479--485},
  publisher    = {Royal Society},
  series       = {Royal Society of London. Proceedings B. Biological Sciences},
  title        = {Association between major histocompatibility complex class IIB alleles and resistance to Aeromonas salmonicida in Atlantic salmon},
  url          = {http://dx.doi.org/10.1098/rspb.2000.1378},
  volume       = {268},
  year         = {2001},
}