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Molecular characterization and evolution of pheromone binding protein genes in Agrotis moths

Abraham, David LU ; Löfstedt, Christer LU and Picimbon, Jean-Francois LU (2005) In Insect Biochemistry and Molecular Biology 35(10). p.1100-1111
Abstract
Pheromone-binding proteins (PBPs) are soluble transporter proteins that increase the capture and the solubilization of pheromone molecules in the lymph surrounding the olfactory receptors. A polymerase chain reaction-based method was used to identify PBP genes in Agrotis species for an evolutionary genomic study of noctuid moth PBPs. From genomic DNA we determined the structure of different PBP genes in the two closely related species, Agrotis ipsilon and A. segetum. In all, we clearly identified four genes (Aips-1, Aips-2, Aseg-1 and Asey-2) that represent two distinct PBP orthology groups. We found that the four genes have the same exon-intron structure and that they comprise three exons and two introns but differ in length mainly... (More)
Pheromone-binding proteins (PBPs) are soluble transporter proteins that increase the capture and the solubilization of pheromone molecules in the lymph surrounding the olfactory receptors. A polymerase chain reaction-based method was used to identify PBP genes in Agrotis species for an evolutionary genomic study of noctuid moth PBPs. From genomic DNA we determined the structure of different PBP genes in the two closely related species, Agrotis ipsilon and A. segetum. In all, we clearly identified four genes (Aips-1, Aips-2, Aseg-1 and Asey-2) that represent two distinct PBP orthology groups. We found that the four genes have the same exon-intron structure and that they comprise three exons and two introns but differ in length mainly in the second intron. The three exons of Aseg-2 and Aips-2 have the same lengths but both intron I and intron 2 differ in length between the genes. In contrast, Aips-1 and Aseg-1 show dissimilarity only in the length of intron 2. Interestingly, introns 1 and 2 are inserted in the same positions in the Aips-1, Aips-2, Aseg-1 and Aseg-2 genes. These findings show that the Agrotis PBP genes have common ancestry and probably originate from gene duplication before the speciation of ipsilon and segetum. We found that expression of Aips-1/Aseg-1 and Aips-2/ Aseg-2 is antennal-specific, but expression is not restricted to the inale antennae. (c) 2005 Elsevier Ltd. All rights reserved. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Insect Biochemistry and Molecular Biology
volume
35
issue
10
pages
1100 - 1111
publisher
Elsevier
external identifiers
  • wos:000231489600003
  • pmid:16102416
  • scopus:23444436197
ISSN
1879-0240
DOI
10.1016/j.ibmb.2005.05.002
language
English
LU publication?
yes
id
aa7f3d4b-6eae-46e3-b4fc-a62768a3e45c (old id 146110)
date added to LUP
2007-06-26 16:08:05
date last changed
2017-11-19 04:10:55
@article{aa7f3d4b-6eae-46e3-b4fc-a62768a3e45c,
  abstract     = {Pheromone-binding proteins (PBPs) are soluble transporter proteins that increase the capture and the solubilization of pheromone molecules in the lymph surrounding the olfactory receptors. A polymerase chain reaction-based method was used to identify PBP genes in <i>Agrotis</i> species for an evolutionary genomic study of noctuid moth PBPs. From genomic DNA we determined the structure of different PBP genes in the two closely related species, Agrotis ipsilon and A. segetum. In all, we clearly identified four genes (Aips-1, Aips-2, Aseg-1 and Asey-2) that represent two distinct PBP orthology groups. We found that the four genes have the same exon-intron structure and that they comprise three exons and two introns but differ in length mainly in the second intron. The three exons of Aseg-2 and Aips-2 have the same lengths but both intron I and intron 2 differ in length between the genes. In contrast, Aips-1 and Aseg-1 show dissimilarity only in the length of intron 2. Interestingly, introns 1 and 2 are inserted in the same positions in the Aips-1, Aips-2, Aseg-1 and Aseg-2 genes. These findings show that the Agrotis PBP genes have common ancestry and probably originate from gene duplication before the speciation of ipsilon and segetum. We found that expression of Aips-1/Aseg-1 and Aips-2/ Aseg-2 is antennal-specific, but expression is not restricted to the inale antennae. (c) 2005 Elsevier Ltd. All rights reserved.},
  author       = {Abraham, David and Löfstedt, Christer and Picimbon, Jean-Francois},
  issn         = {1879-0240},
  language     = {eng},
  number       = {10},
  pages        = {1100--1111},
  publisher    = {Elsevier},
  series       = {Insect Biochemistry and Molecular Biology},
  title        = {Molecular characterization and evolution of pheromone binding protein genes in <i>Agrotis</i> moths},
  url          = {http://dx.doi.org/10.1016/j.ibmb.2005.05.002},
  volume       = {35},
  year         = {2005},
}