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Formation of carbonated apatite particles from a supersaturated inorganic blood serum model

Sandin, Karin ; Kloo, Lars ; Nevsten, Pernilla LU ; Wallenberg, Reine LU and Olsson, Lars-Fride (2009) In Journal of Materials Science: Materials in Medicine 20(8). p.1677-1687
Abstract
Pathological calcification is common among for instance dialysis patients, and this causes an increase in mortality risk. An elevated serum phosphate concentration among those patients strongly correlates to this increase. In this work investigations of the conditions, composition, crystallinity and morphology of in vitro calcification are performed and related to results from in vivo studies. The study was performed under conditions mimicking physiological ones, i.e. a pH around 7.40, a temperature of 37A degrees C, an ionic strength of 150 mM and ion concentrations close to those in human serum including the effects of elevated phosphate concentrations. The course of precipitation involves an initial precipitate that subsequently... (More)
Pathological calcification is common among for instance dialysis patients, and this causes an increase in mortality risk. An elevated serum phosphate concentration among those patients strongly correlates to this increase. In this work investigations of the conditions, composition, crystallinity and morphology of in vitro calcification are performed and related to results from in vivo studies. The study was performed under conditions mimicking physiological ones, i.e. a pH around 7.40, a temperature of 37A degrees C, an ionic strength of 150 mM and ion concentrations close to those in human serum including the effects of elevated phosphate concentrations. The course of precipitation involves an initial precipitate that subsequently re-dissolves to give another precipitate, in accordance with the well-known Ostwald ripening theory. The final bulk precipitate consists of a macroscopically amorphous carbonated apatite. The amorphous apatite is formed from assemblies of spherical particles in the mu m range, in turn composed of nano-crystalline needles of about 10 x 100 nm. Even the initially formed precipitate, as well as a small amount of precipitate that occurs on the liquid surface, consist of a carbonated calcium phosphate. The in vitro observed carbonated apatite bears strong resemblance to in vivo cardiovascular calcification known from literature. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Materials Science: Materials in Medicine
volume
20
issue
8
pages
1677 - 1687
publisher
Springer
external identifiers
  • wos:000268103300012
  • scopus:68149124109
  • pmid:19347257
ISSN
1573-4838
DOI
10.1007/s10856-009-3735-z
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Polymer and Materials Chemistry (LTH) (011001041)
id
fc0c30b8-89b3-4030-b66f-68a44516bbea (old id 1461513)
date added to LUP
2016-04-01 14:08:34
date last changed
2025-01-17 14:35:05
@article{fc0c30b8-89b3-4030-b66f-68a44516bbea,
  abstract     = {{Pathological calcification is common among for instance dialysis patients, and this causes an increase in mortality risk. An elevated serum phosphate concentration among those patients strongly correlates to this increase. In this work investigations of the conditions, composition, crystallinity and morphology of in vitro calcification are performed and related to results from in vivo studies. The study was performed under conditions mimicking physiological ones, i.e. a pH around 7.40, a temperature of 37A degrees C, an ionic strength of 150 mM and ion concentrations close to those in human serum including the effects of elevated phosphate concentrations. The course of precipitation involves an initial precipitate that subsequently re-dissolves to give another precipitate, in accordance with the well-known Ostwald ripening theory. The final bulk precipitate consists of a macroscopically amorphous carbonated apatite. The amorphous apatite is formed from assemblies of spherical particles in the mu m range, in turn composed of nano-crystalline needles of about 10 x 100 nm. Even the initially formed precipitate, as well as a small amount of precipitate that occurs on the liquid surface, consist of a carbonated calcium phosphate. The in vitro observed carbonated apatite bears strong resemblance to in vivo cardiovascular calcification known from literature.}},
  author       = {{Sandin, Karin and Kloo, Lars and Nevsten, Pernilla and Wallenberg, Reine and Olsson, Lars-Fride}},
  issn         = {{1573-4838}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{1677--1687}},
  publisher    = {{Springer}},
  series       = {{Journal of Materials Science: Materials in Medicine}},
  title        = {{Formation of carbonated apatite particles from a supersaturated inorganic blood serum model}},
  url          = {{http://dx.doi.org/10.1007/s10856-009-3735-z}},
  doi          = {{10.1007/s10856-009-3735-z}},
  volume       = {{20}},
  year         = {{2009}},
}