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Two novel IgG endopeptidases of Streptococcus equi

Hulting, Greta; Flock, Margareta; Frykberg, Lars; Lannergård, Jonas LU ; Flock, Jan-Ingmar and Guss, Bengt (2009) In FEMS Microbiology Letters 298(1). p.44-50
Abstract
Streptococcus equi ssp. equi causes strangles, a highly contagious and serious disease in the upper respiratory tract of horses. Streptococcus equi ssp. zooepidemicus, another subspecies of this genus, is regarded as an opportunistic commensal in horses. The present study describes the characterization of two novel immunoglobulin G (IgG) endopeptidases of these subspecies, IdeE2 and IdeZ2. Both enzymes display sequence similarities with two previously characterized IgG endopeptidases, IdeE of S. equi ssp. equi and IdeZ of S. equi ssp. zooepidemicus. IdeE2 and IdeZ2 display high substrate-specificity in comparison with IdeE and IdeZ, as they both completely cleave horse IgG, while the activity against IgG from mouse, rabbit, cat, cow, sheep... (More)
Streptococcus equi ssp. equi causes strangles, a highly contagious and serious disease in the upper respiratory tract of horses. Streptococcus equi ssp. zooepidemicus, another subspecies of this genus, is regarded as an opportunistic commensal in horses. The present study describes the characterization of two novel immunoglobulin G (IgG) endopeptidases of these subspecies, IdeE2 and IdeZ2. Both enzymes display sequence similarities with two previously characterized IgG endopeptidases, IdeE of S. equi ssp. equi and IdeZ of S. equi ssp. zooepidemicus. IdeE2 and IdeZ2 display high substrate-specificity in comparison with IdeE and IdeZ, as they both completely cleave horse IgG, while the activity against IgG from mouse, rabbit, cat, cow, sheep and goat is low or absent. The potential use of IdeE and IdeE2 as vaccine components was studied in a mouse infection model. In this vaccination and challenge study, both enzymes induced protection against S. equi ssp. equi infection. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
vaccine, IgG endopeptidases, IdeS, IdeZ, Streptococcus equi, IdeE
in
FEMS Microbiology Letters
volume
298
issue
1
pages
44 - 50
publisher
Elsevier
external identifiers
  • wos:000268170700006
  • scopus:68149128414
ISSN
1574-6968
DOI
10.1111/j.1574-6968.2009.01698.x
language
English
LU publication?
yes
id
e623d9fe-8e34-4354-8435-b0617211e392 (old id 1461631)
date added to LUP
2009-08-31 10:53:38
date last changed
2017-08-20 03:59:21
@article{e623d9fe-8e34-4354-8435-b0617211e392,
  abstract     = {Streptococcus equi ssp. equi causes strangles, a highly contagious and serious disease in the upper respiratory tract of horses. Streptococcus equi ssp. zooepidemicus, another subspecies of this genus, is regarded as an opportunistic commensal in horses. The present study describes the characterization of two novel immunoglobulin G (IgG) endopeptidases of these subspecies, IdeE2 and IdeZ2. Both enzymes display sequence similarities with two previously characterized IgG endopeptidases, IdeE of S. equi ssp. equi and IdeZ of S. equi ssp. zooepidemicus. IdeE2 and IdeZ2 display high substrate-specificity in comparison with IdeE and IdeZ, as they both completely cleave horse IgG, while the activity against IgG from mouse, rabbit, cat, cow, sheep and goat is low or absent. The potential use of IdeE and IdeE2 as vaccine components was studied in a mouse infection model. In this vaccination and challenge study, both enzymes induced protection against S. equi ssp. equi infection.},
  author       = {Hulting, Greta and Flock, Margareta and Frykberg, Lars and Lannergård, Jonas and Flock, Jan-Ingmar and Guss, Bengt},
  issn         = {1574-6968},
  keyword      = {vaccine,IgG endopeptidases,IdeS,IdeZ,Streptococcus equi,IdeE},
  language     = {eng},
  number       = {1},
  pages        = {44--50},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Letters},
  title        = {Two novel IgG endopeptidases of Streptococcus equi},
  url          = {http://dx.doi.org/10.1111/j.1574-6968.2009.01698.x},
  volume       = {298},
  year         = {2009},
}