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Changes in Membrane Phospholipids as a Mechanistic Explanation for Decreased Freeze Tolerance in Earthworms Exposed to Sublethal Copper Concentrations

Bindesbol, Anne-Mette; Bayley, Mark; Damgaard, Christian; Hedlund, Katarina LU and Holmstrup, Martin (2009) In Environmental Science & Technology 43(14). p.5495-5500
Abstract
At low temperature, cell membrane functionality depends on adjustments of membrane phospholipid fatty acid (PLFA) composition. We here test the hypothesis that previous exposure to copper (Cu) may deteriorate tolerance to freezing temperatures because of Cu-induced changes of PLFA composition of cell membranes in the freeze-tolerant earthworm Dendrobaena octaedra. Cu levels and freezing temperatures were varied in a full factorial design. We measured PLFA composition and lipid peroxidation. A highly significant interaction was observed between subzero temperatures and Cu concentrations above 120 mg/kg dry soil. Lipid peroxidation increased slightly in worms exposed to Cu. In particular, the analysis showed that Cu had a significant... (More)
At low temperature, cell membrane functionality depends on adjustments of membrane phospholipid fatty acid (PLFA) composition. We here test the hypothesis that previous exposure to copper (Cu) may deteriorate tolerance to freezing temperatures because of Cu-induced changes of PLFA composition of cell membranes in the freeze-tolerant earthworm Dendrobaena octaedra. Cu levels and freezing temperatures were varied in a full factorial design. We measured PLFA composition and lipid peroxidation. A highly significant interaction was observed between subzero temperatures and Cu concentrations above 120 mg/kg dry soil. Lipid peroxidation increased slightly in worms exposed to Cu. In particular, the analysis showed that Cu had a significant negative effect on the polyunsaturated PLFA, linoleic acid (18:2 omega 6,9), which has previously been reported to correlate positively (R-2 = 0.92) with freeze tolerance in D. octaedra. This supports our hypothesis that reduced tolerance to freezing temperatures in Cu-exposed worms may be due to membrane damage. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Environmental Science & Technology
volume
43
issue
14
pages
5495 - 5500
publisher
The American Chemical Society
external identifiers
  • wos:000268138000054
  • scopus:67650483694
ISSN
1520-5851
DOI
10.1021/es900898y
language
English
LU publication?
yes
id
d127567d-2071-4748-b996-09807a0c4a66 (old id 1461698)
date added to LUP
2009-08-21 14:53:43
date last changed
2017-08-27 04:28:20
@article{d127567d-2071-4748-b996-09807a0c4a66,
  abstract     = {At low temperature, cell membrane functionality depends on adjustments of membrane phospholipid fatty acid (PLFA) composition. We here test the hypothesis that previous exposure to copper (Cu) may deteriorate tolerance to freezing temperatures because of Cu-induced changes of PLFA composition of cell membranes in the freeze-tolerant earthworm Dendrobaena octaedra. Cu levels and freezing temperatures were varied in a full factorial design. We measured PLFA composition and lipid peroxidation. A highly significant interaction was observed between subzero temperatures and Cu concentrations above 120 mg/kg dry soil. Lipid peroxidation increased slightly in worms exposed to Cu. In particular, the analysis showed that Cu had a significant negative effect on the polyunsaturated PLFA, linoleic acid (18:2 omega 6,9), which has previously been reported to correlate positively (R-2 = 0.92) with freeze tolerance in D. octaedra. This supports our hypothesis that reduced tolerance to freezing temperatures in Cu-exposed worms may be due to membrane damage.},
  author       = {Bindesbol, Anne-Mette and Bayley, Mark and Damgaard, Christian and Hedlund, Katarina and Holmstrup, Martin},
  issn         = {1520-5851},
  language     = {eng},
  number       = {14},
  pages        = {5495--5500},
  publisher    = {The American Chemical Society},
  series       = {Environmental Science & Technology},
  title        = {Changes in Membrane Phospholipids as a Mechanistic Explanation for Decreased Freeze Tolerance in Earthworms Exposed to Sublethal Copper Concentrations},
  url          = {http://dx.doi.org/10.1021/es900898y},
  volume       = {43},
  year         = {2009},
}