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Spatial patterns of gene expression in the extramatrical mycelium and mycorrhizal root tips formed by the ectomycorrhizal fungus Paxillus involutus in association with birch (Betula pendula) seedlings in soil microcosms

Wright, Derek LU ; Johansson, Tomas LU ; Le Quéré, Antoine LU ; Söderström, Bengt LU and Tunlid, Anders LU (2005) In New Phytologist 167(2). p.579-596
Abstract
(.)Functional compartmentation of the extramatrical mycelium of ectomycorrhizal (ECM) fungi is considered important for the operation of ECM associations, although the molecular basis is poorly characterized. (.)Global gene expression profiles of mycelium colonizing an ammonium sulphate ((NH4)(2)SO4) nutrient patch, rhizomorphs and ECM root tips of the Betula pendula-Paxillus involutus association were compared by cDNA microarray analysis. (.)The expression profiles of rhizomorphs and nutrient patch mycelium were similar to each other but distinctly different from that of mycorrhizal tips. Statistical analyses revealed 337 of 1075 fungal genes differentially regulated among these three tissues. Clusters of genes exhibiting distinct... (More)
(.)Functional compartmentation of the extramatrical mycelium of ectomycorrhizal (ECM) fungi is considered important for the operation of ECM associations, although the molecular basis is poorly characterized. (.)Global gene expression profiles of mycelium colonizing an ammonium sulphate ((NH4)(2)SO4) nutrient patch, rhizomorphs and ECM root tips of the Betula pendula-Paxillus involutus association were compared by cDNA microarray analysis. (.)The expression profiles of rhizomorphs and nutrient patch mycelium were similar to each other but distinctly different from that of mycorrhizal tips. Statistical analyses revealed 337 of 1075 fungal genes differentially regulated among these three tissues. Clusters of genes exhibiting distinct expression patterns within specific tissues were identified. Genes implicated in the glutamine synthetase/glutamate synthase (GS/GOGAT) and urea cycles, and the provision of carbon skeletons for ammonium assimilation via beta-oxidation and the glyoxylate cycle, were highly expressed in rhizomorph and nutrient patch mycelium. Genes implicated in vesicular transport, cytoskeleton organization and morphogenesis and protein degradation were also differentially expressed. (.)Differential expression of genes among the extramatrical mycelium and mycorrhizal tips indicates functional specialization of tissues forming ECM associations. (c) New Phytologist ( 2005). (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
New Phytologist
volume
167
issue
2
pages
579 - 596
publisher
Wiley-Blackwell
external identifiers
  • wos:000230286800024
  • pmid:15998408
  • scopus:25144441284
ISSN
1469-8137
DOI
10.1111/j.1469-8137.2005.01441.x
language
English
LU publication?
yes
id
7adc86c0-f099-4eac-9028-c01254dc2943 (old id 146780)
date added to LUP
2007-07-02 07:42:05
date last changed
2017-10-29 03:37:01
@article{7adc86c0-f099-4eac-9028-c01254dc2943,
  abstract     = {(.)Functional compartmentation of the extramatrical mycelium of ectomycorrhizal (ECM) fungi is considered important for the operation of ECM associations, although the molecular basis is poorly characterized. (.)Global gene expression profiles of mycelium colonizing an ammonium sulphate ((NH4)(2)SO4) nutrient patch, rhizomorphs and ECM root tips of the Betula pendula-Paxillus involutus association were compared by cDNA microarray analysis. (.)The expression profiles of rhizomorphs and nutrient patch mycelium were similar to each other but distinctly different from that of mycorrhizal tips. Statistical analyses revealed 337 of 1075 fungal genes differentially regulated among these three tissues. Clusters of genes exhibiting distinct expression patterns within specific tissues were identified. Genes implicated in the glutamine synthetase/glutamate synthase (GS/GOGAT) and urea cycles, and the provision of carbon skeletons for ammonium assimilation via beta-oxidation and the glyoxylate cycle, were highly expressed in rhizomorph and nutrient patch mycelium. Genes implicated in vesicular transport, cytoskeleton organization and morphogenesis and protein degradation were also differentially expressed. (.)Differential expression of genes among the extramatrical mycelium and mycorrhizal tips indicates functional specialization of tissues forming ECM associations. (c) New Phytologist ( 2005).},
  author       = {Wright, Derek and Johansson, Tomas and Le Quéré, Antoine and Söderström, Bengt and Tunlid, Anders},
  issn         = {1469-8137},
  language     = {eng},
  number       = {2},
  pages        = {579--596},
  publisher    = {Wiley-Blackwell},
  series       = {New Phytologist},
  title        = {Spatial patterns of gene expression in the extramatrical mycelium and mycorrhizal root tips formed by the ectomycorrhizal fungus Paxillus involutus in association with birch (Betula pendula) seedlings in soil microcosms},
  url          = {http://dx.doi.org/10.1111/j.1469-8137.2005.01441.x},
  volume       = {167},
  year         = {2005},
}