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C-13 incorporation into signature fatty acids as an assay for carbon allocation in arbuscular mycorrhiza

Olsson, Pål Axel LU ; van Aarle, I M; Gavito, M E; Bengtson, Per LU and Bengtsson, Göran LU (2005) In Applied and Environmental Microbiology 71(5). p.2592-2599
Abstract
The ubiquitous arbuscular mycorrhizal fungi consume significant amounts of plant assimilated C, but this C flow has been difficult to quantify. The neutral lipid fatty acid 16:1ω 5 is a quantitative signature for most arbuscular mycorrhizal fungi in roots and soil. We measured carbon transfer from four plant species to the arbuscular mycorrhizal fungus Glomus intraradices by estimating C-13 enrichment of 16:1ω 5 and compared it with C-13 enrichment of total root and mycelial C. Carbon allocation to mycelia was detected within 1 day in monoxenic arbuscular mycorrhizal root cultures labeled with [C-13] glucose. The 13 C enrichment of neutral lipid fatty acid 16:1ω 5 extracted from roots increased from 0.14% 1 day after labeling to 2.2% 7... (More)
The ubiquitous arbuscular mycorrhizal fungi consume significant amounts of plant assimilated C, but this C flow has been difficult to quantify. The neutral lipid fatty acid 16:1ω 5 is a quantitative signature for most arbuscular mycorrhizal fungi in roots and soil. We measured carbon transfer from four plant species to the arbuscular mycorrhizal fungus Glomus intraradices by estimating C-13 enrichment of 16:1ω 5 and compared it with C-13 enrichment of total root and mycelial C. Carbon allocation to mycelia was detected within 1 day in monoxenic arbuscular mycorrhizal root cultures labeled with [C-13] glucose. The 13 C enrichment of neutral lipid fatty acid 16:1ω 5 extracted from roots increased from 0.14% 1 day after labeling to 2.2% 7 days after labeling. The colonized roots usually were more enriched for C-13 in the arbuscular mycorrhizal fungal neutral lipid fatty acid 16:1w5 than for the root specific neutral lipid fatty acid 18:2ω 6,9. We labeled plant assimilates by using (CO2)-C-13 in whole-plant experiments. The extraradical mycelium often was more enriched for C-13 than was the intraradical mycelium, suggesting rapid translocation of carbon to and more active growth by the extraradical mycelium. Since there was a good correlation between 13C enrichment in neutral lipid fatty acid 16:1ω 5 and total C-13 in extraradical mycelia in different systems (r(2) = 0.94), we propose that the total amount of labeled C in intraradical and extraradical mycelium can be calculated from the C-13 enrichment of 16:1ω 5. The method described enables evaluation of C flow from plants to arbuscular mycorrhizal fungi to be made without extraction, purification and identification of fungal mycelia. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied and Environmental Microbiology
volume
71
issue
5
pages
2592 - 2599
publisher
American Society for Microbiology
external identifiers
  • wos:000229105300050
  • pmid:15870350
  • scopus:18444375576
ISSN
0099-2240
DOI
10.1128/AEM.71.5.2592-2599.2005
language
English
LU publication?
yes
id
e6d8bf89-b292-4834-aa53-c60e5d999d0a (old id 146797)
date added to LUP
2007-06-27 11:15:13
date last changed
2017-10-22 03:36:25
@article{e6d8bf89-b292-4834-aa53-c60e5d999d0a,
  abstract     = {The ubiquitous arbuscular mycorrhizal fungi consume significant amounts of plant assimilated C, but this C flow has been difficult to quantify. The neutral lipid fatty acid 16:1ω 5 is a quantitative signature for most arbuscular mycorrhizal fungi in roots and soil. We measured carbon transfer from four plant species to the arbuscular mycorrhizal fungus Glomus intraradices by estimating C-13 enrichment of 16:1ω 5 and compared it with C-13 enrichment of total root and mycelial C. Carbon allocation to mycelia was detected within 1 day in monoxenic arbuscular mycorrhizal root cultures labeled with [C-13] glucose. The 13 C enrichment of neutral lipid fatty acid 16:1ω 5 extracted from roots increased from 0.14% 1 day after labeling to 2.2% 7 days after labeling. The colonized roots usually were more enriched for C-13 in the arbuscular mycorrhizal fungal neutral lipid fatty acid 16:1w5 than for the root specific neutral lipid fatty acid 18:2ω 6,9. We labeled plant assimilates by using (CO2)-C-13 in whole-plant experiments. The extraradical mycelium often was more enriched for C-13 than was the intraradical mycelium, suggesting rapid translocation of carbon to and more active growth by the extraradical mycelium. Since there was a good correlation between 13C enrichment in neutral lipid fatty acid 16:1ω 5 and total C-13 in extraradical mycelia in different systems (r(2) = 0.94), we propose that the total amount of labeled C in intraradical and extraradical mycelium can be calculated from the C-13 enrichment of 16:1ω 5. The method described enables evaluation of C flow from plants to arbuscular mycorrhizal fungi to be made without extraction, purification and identification of fungal mycelia.},
  author       = {Olsson, Pål Axel and van Aarle, I M and Gavito, M E and Bengtson, Per and Bengtsson, Göran},
  issn         = {0099-2240},
  language     = {eng},
  number       = {5},
  pages        = {2592--2599},
  publisher    = {American Society for Microbiology},
  series       = {Applied and Environmental Microbiology},
  title        = {C-13 incorporation into signature fatty acids as an assay for carbon allocation in arbuscular mycorrhiza},
  url          = {http://dx.doi.org/10.1128/AEM.71.5.2592-2599.2005},
  volume       = {71},
  year         = {2005},
}