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Adhesion of conidia and germlings of the plant pathogenic fungus Bipolaris sorokiniana to solid surfaces

Apoga, D; Jansson, Hans-Börje LU and Tunlid, Anders LU (2001) In Mycological Research1989-01-01+01:002010-01-01+01:00 105(10). p.1251-1260
Abstract
Soon after coming in contact with its host, the plant pathogenic fungus Bipolaris sorokiniana produces an extracellular material that appears to be important for adhering conidia and germlings to the host surface. To further understand this step of the infection, the adhesion of B. sorokiniana to artificial solid surfaces was examined. On a hydrophobic (polystyrene) surface adhesion occurred in two stages, the first by conidia and the second by germlings. Conidial adhesion occurred shortly (0-1 h) after hydration. The conidia were easily detached by increasing the shear force and including detergents in the washing buffer. As conidia were hydrophobic, these observations indicate that conidial adhesion to polystyrene is due to weak,... (More)
Soon after coming in contact with its host, the plant pathogenic fungus Bipolaris sorokiniana produces an extracellular material that appears to be important for adhering conidia and germlings to the host surface. To further understand this step of the infection, the adhesion of B. sorokiniana to artificial solid surfaces was examined. On a hydrophobic (polystyrene) surface adhesion occurred in two stages, the first by conidia and the second by germlings. Conidial adhesion occurred shortly (0-1 h) after hydration. The conidia were easily detached by increasing the shear force and including detergents in the washing buffer. As conidia were hydrophobic, these observations indicate that conidial adhesion to polystyrene is due to weak, hydrophobic interaction. The second stage of adhesion was accompanied by conidial germination and occurred 1-2 h after hydration and contact with the surface. Concomitant with the delayed adhesion, the fungus produced an extracellular matrix (ECM). The adhesion of germlings was firm and surface-unspecific since they adhered to both hydrophobic and hydrophilic (glass) surfaces, Except for strong bases, hydrochloric acid and broad-specificity proteases (including Pronase E), none of the hydrolytic enzymes, electrolyte solutions, ionic and hydrophobic detergents and organic solvents removed germlings from the solid surfaces. The adhesion of germlings incubated in the presence of the protein glycosylation inhibitor tunicamycin or the lectins Con A (Concanavalin A) and GNA (from Galanthus nivalis) was significantly reduced, which indicates the involvement of surface glycoproteins in this process. The surface proteins of germlings were labelled with I-125, extracted and analysed by two-dimensional gel electrophoresis. This revealed about 40 surface proteins over a wide pH range (4-10) with molecular masses between 10 and 100 kDa. (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
in
Mycological Research1989-01-01+01:002010-01-01+01:00
volume
105
issue
10
pages
1251 - 1260
publisher
Elsevier
external identifiers
  • scopus:0035198015
ISSN
1469-8102
DOI
10.1017/S0953756201004683
language
English
LU publication?
yes
id
9b1d0c5f-fecf-42c5-94c5-15d4c939ff12 (old id 146937)
date added to LUP
2007-06-29 09:02:53
date last changed
2018-05-29 09:56:24
@article{9b1d0c5f-fecf-42c5-94c5-15d4c939ff12,
  abstract     = {Soon after coming in contact with its host, the plant pathogenic fungus Bipolaris sorokiniana produces an extracellular material that appears to be important for adhering conidia and germlings to the host surface. To further understand this step of the infection, the adhesion of B. sorokiniana to artificial solid surfaces was examined. On a hydrophobic (polystyrene) surface adhesion occurred in two stages, the first by conidia and the second by germlings. Conidial adhesion occurred shortly (0-1 h) after hydration. The conidia were easily detached by increasing the shear force and including detergents in the washing buffer. As conidia were hydrophobic, these observations indicate that conidial adhesion to polystyrene is due to weak, hydrophobic interaction. The second stage of adhesion was accompanied by conidial germination and occurred 1-2 h after hydration and contact with the surface. Concomitant with the delayed adhesion, the fungus produced an extracellular matrix (ECM). The adhesion of germlings was firm and surface-unspecific since they adhered to both hydrophobic and hydrophilic (glass) surfaces, Except for strong bases, hydrochloric acid and broad-specificity proteases (including Pronase E), none of the hydrolytic enzymes, electrolyte solutions, ionic and hydrophobic detergents and organic solvents removed germlings from the solid surfaces. The adhesion of germlings incubated in the presence of the protein glycosylation inhibitor tunicamycin or the lectins Con A (Concanavalin A) and GNA (from Galanthus nivalis) was significantly reduced, which indicates the involvement of surface glycoproteins in this process. The surface proteins of germlings were labelled with I-125, extracted and analysed by two-dimensional gel electrophoresis. This revealed about 40 surface proteins over a wide pH range (4-10) with molecular masses between 10 and 100 kDa.},
  author       = {Apoga, D and Jansson, Hans-Börje and Tunlid, Anders},
  issn         = {1469-8102},
  language     = {eng},
  number       = {10},
  pages        = {1251--1260},
  publisher    = {Elsevier},
  series       = {Mycological Research1989-01-01+01:002010-01-01+01:00},
  title        = {Adhesion of conidia and germlings of the plant pathogenic fungus Bipolaris sorokiniana to solid surfaces},
  url          = {http://dx.doi.org/10.1017/S0953756201004683},
  volume       = {105},
  year         = {2001},
}