Advanced

Adaptation of a rapid and economical microcentrifugation method to measure thymidine and leucine incorporation by soil bacteria

Bååth, Erland LU ; Pettersson, Marie LU and Söderberg, Katarina LU (2001) In Soil Biology & Biochemistry 33(11). p.1571-1574
Abstract
A simplified method using centrifugation and microcentrifuge tubes instead of filtration to measure bacterial activity using thymidine or leucine incorporation, originally used in aquatic habitats, has been adapted for soil. A final step employing hot NaOH to solubilize macromolecules before adding scintillation fluid was necessary to achieve the same incorporation rates as those in the filtration technique. The microcentrifugation technique has several advantages, including being less costly, less laborious and having lower zero-time controls. The samples could also be stored for at least 2 weeks after incorporation was stopped by adding TCA. The microcentrifugation technique would therefore be most useful when a large number of samples,... (More)
A simplified method using centrifugation and microcentrifuge tubes instead of filtration to measure bacterial activity using thymidine or leucine incorporation, originally used in aquatic habitats, has been adapted for soil. A final step employing hot NaOH to solubilize macromolecules before adding scintillation fluid was necessary to achieve the same incorporation rates as those in the filtration technique. The microcentrifugation technique has several advantages, including being less costly, less laborious and having lower zero-time controls. The samples could also be stored for at least 2 weeks after incorporation was stopped by adding TCA. The microcentrifugation technique would therefore be most useful when a large number of samples, some with very low incorporation rates, are to be studied, for example, when studying bacterial community tolerance. (C) 2001 Elsevier Science Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Soil Biology & Biochemistry
volume
33
issue
11
pages
1571 - 1574
publisher
Elsevier
external identifiers
  • scopus:0034839622
ISSN
0038-0717
DOI
10.1016/S0038-0717(01)00073-6
language
English
LU publication?
yes
id
c7e8dbb6-62ac-4ab9-b344-d3300129820a (old id 146945)
date added to LUP
2007-06-29 09:17:59
date last changed
2018-07-15 04:13:23
@article{c7e8dbb6-62ac-4ab9-b344-d3300129820a,
  abstract     = {A simplified method using centrifugation and microcentrifuge tubes instead of filtration to measure bacterial activity using thymidine or leucine incorporation, originally used in aquatic habitats, has been adapted for soil. A final step employing hot NaOH to solubilize macromolecules before adding scintillation fluid was necessary to achieve the same incorporation rates as those in the filtration technique. The microcentrifugation technique has several advantages, including being less costly, less laborious and having lower zero-time controls. The samples could also be stored for at least 2 weeks after incorporation was stopped by adding TCA. The microcentrifugation technique would therefore be most useful when a large number of samples, some with very low incorporation rates, are to be studied, for example, when studying bacterial community tolerance. (C) 2001 Elsevier Science Ltd. All rights reserved.},
  author       = {Bååth, Erland and Pettersson, Marie and Söderberg, Katarina},
  issn         = {0038-0717},
  language     = {eng},
  number       = {11},
  pages        = {1571--1574},
  publisher    = {Elsevier},
  series       = {Soil Biology & Biochemistry},
  title        = {Adaptation of a rapid and economical microcentrifugation method to measure thymidine and leucine incorporation by soil bacteria},
  url          = {http://dx.doi.org/10.1016/S0038-0717(01)00073-6},
  volume       = {33},
  year         = {2001},
}