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Solubilisation and colonisation of wood ash by ectomycorrhizal fungi isolated from a wood ash fertilised spruce forest

Mahmood, Shahid LU ; Finlay, R D; Erland, Susanne LU and Wallander, Håkan LU (2001) In FEMS Microbiology Ecology 35(2). p.151-161
Abstract
In Sweden application of granulated wood ash has been suggested as a method to supplement nutrient loss resulting from harvesting of forest residues for bioenergy production. Mycelia of two ectomycorrhizal fungi Piloderma sp.I and Ha-96-3. were commonly found to colonise ash granules in a wood ash fertilised spruce forest. Thirty-eight fungal isolates were selected from 10 taxa to investigate the possible role of different ectomycorrhizal Fungi in nutrient mobilisation from ash. The taxa were Cenococcum geophilum Fr,, Piloderma croceum Erikss, and Hjortst., Piloderma sp. I, Thelephora terrestris (Ehrenb.) Fr., Tylospora fibrillosa Donk, and five unidentified species, all originating from a wood ash fertilised spruce forest. The isolates... (More)
In Sweden application of granulated wood ash has been suggested as a method to supplement nutrient loss resulting from harvesting of forest residues for bioenergy production. Mycelia of two ectomycorrhizal fungi Piloderma sp.I and Ha-96-3. were commonly found to colonise ash granules in a wood ash fertilised spruce forest. Thirty-eight fungal isolates were selected from 10 taxa to investigate the possible role of different ectomycorrhizal Fungi in nutrient mobilisation from ash. The taxa were Cenococcum geophilum Fr,, Piloderma croceum Erikss, and Hjortst., Piloderma sp. I, Thelephora terrestris (Ehrenb.) Fr., Tylospora fibrillosa Donk, and five unidentified species, all originating from a wood ash fertilised spruce forest. The isolates were tested for their ability to solubilise tricalcium phosphate (TCP) or hardened wood ash (HWA) in vitro. Ha-96-3, P, croceum and Piloderma sp. I were the only taxa which solubilised TCP. Abundant calcium oxalate crystals were formed in TCP and HWA plates with Piloderma sp. I. Ha-96-3 and two isolates of P. croceum produced intermediate amounts of crystals. Ha-96-1 and T. fibrillosa produced low amounts of crystal but no crystal formation was observed by any of the other isolates. Piloderma sp. I from HWA plates had significantly higher concentrations of P, compared to P. croceum or Ha-96-3. Piloderma sp. I and P. croceum were further tested for their ability to colonise wood ash in microcosms containing intact mycorrhizal associations. After 7 months Piloderma sp. I colonised ash amended patches with a dense, mat like mycelium, whereas P. croceum mycelia avoided the ash patches, Possible differences between these fungi in patterns of carbon allocation were investigated by labelling seedlings with (CO2)-C-14. Piloderma sp. I mycelia allocated significantly more C-14 to ash patches than P. croceum. P. croceum allocated relatively more C-14 to control patches than to the ash patches. The possible role of ectomycorrhizal fungi in mobilisation of nutrients from wood ash is discussed. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved. (Less)
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Contribution to journal
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published
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in
FEMS Microbiology Ecology
volume
35
issue
2
pages
151 - 161
publisher
Elsevier
external identifiers
  • scopus:0035075415
ISSN
1574-6941
DOI
10.1016/S0168-6496(00)00124-0
language
English
LU publication?
yes
id
584df734-9556-42a3-baea-23062e559da6 (old id 146968)
date added to LUP
2007-06-29 12:28:31
date last changed
2018-05-29 11:13:57
@article{584df734-9556-42a3-baea-23062e559da6,
  abstract     = {In Sweden application of granulated wood ash has been suggested as a method to supplement nutrient loss resulting from harvesting of forest residues for bioenergy production. Mycelia of two ectomycorrhizal fungi Piloderma sp.I and Ha-96-3. were commonly found to colonise ash granules in a wood ash fertilised spruce forest. Thirty-eight fungal isolates were selected from 10 taxa to investigate the possible role of different ectomycorrhizal Fungi in nutrient mobilisation from ash. The taxa were Cenococcum geophilum Fr,, Piloderma croceum Erikss, and Hjortst., Piloderma sp. I, Thelephora terrestris (Ehrenb.) Fr., Tylospora fibrillosa Donk, and five unidentified species, all originating from a wood ash fertilised spruce forest. The isolates were tested for their ability to solubilise tricalcium phosphate (TCP) or hardened wood ash (HWA) in vitro. Ha-96-3, P, croceum and Piloderma sp. I were the only taxa which solubilised TCP. Abundant calcium oxalate crystals were formed in TCP and HWA plates with Piloderma sp. I. Ha-96-3 and two isolates of P. croceum produced intermediate amounts of crystals. Ha-96-1 and T. fibrillosa produced low amounts of crystal but no crystal formation was observed by any of the other isolates. Piloderma sp. I from HWA plates had significantly higher concentrations of P, compared to P. croceum or Ha-96-3. Piloderma sp. I and P. croceum were further tested for their ability to colonise wood ash in microcosms containing intact mycorrhizal associations. After 7 months Piloderma sp. I colonised ash amended patches with a dense, mat like mycelium, whereas P. croceum mycelia avoided the ash patches, Possible differences between these fungi in patterns of carbon allocation were investigated by labelling seedlings with (CO2)-C-14. Piloderma sp. I mycelia allocated significantly more C-14 to ash patches than P. croceum. P. croceum allocated relatively more C-14 to control patches than to the ash patches. The possible role of ectomycorrhizal fungi in mobilisation of nutrients from wood ash is discussed. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.},
  author       = {Mahmood, Shahid and Finlay, R D and Erland, Susanne and Wallander, Håkan},
  issn         = {1574-6941},
  language     = {eng},
  number       = {2},
  pages        = {151--161},
  publisher    = {Elsevier},
  series       = {FEMS Microbiology Ecology},
  title        = {Solubilisation and colonisation of wood ash by ectomycorrhizal fungi isolated from a wood ash fertilised spruce forest},
  url          = {http://dx.doi.org/10.1016/S0168-6496(00)00124-0},
  volume       = {35},
  year         = {2001},
}