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Microscopic detection of phosphatase activity of saprophytic and arbuscular mycorrhizal fungi using a fluorogenic substrate

van Aarle, Ingrid LU ; Olsson, Pål Axel LU and Söderström, Bengt LU (2001) In Mycologia 93(1). p.17-24
Abstract
The location and activity of saprophytic and arbuscular mycorrhizal (NM) fungal phosphatases have been investigated with a fluorogenic substrate (ELF) that forms a fluorescent crystalline precipitate at the site of phosphatase activity. This ELF substrate method was compared with other detection methods such as fast blue RR salt staining and p-nitrophenyl phosphate. The ELF substrate was successfully used to determine the location of phosphatase activity in AM fungal hyphae, and to visualize the active AM fungal colonization of roots. The substrate was taken up by fungal hyphae, when acid and alkaline buffers were used, and internal phosphatase activity was visualized. The release of both acid and alkaline phosphatases by a saprophytic... (More)
The location and activity of saprophytic and arbuscular mycorrhizal (NM) fungal phosphatases have been investigated with a fluorogenic substrate (ELF) that forms a fluorescent crystalline precipitate at the site of phosphatase activity. This ELF substrate method was compared with other detection methods such as fast blue RR salt staining and p-nitrophenyl phosphate. The ELF substrate was successfully used to determine the location of phosphatase activity in AM fungal hyphae, and to visualize the active AM fungal colonization of roots. The substrate was taken up by fungal hyphae, when acid and alkaline buffers were used, and internal phosphatase activity was visualized. The release of both acid and alkaline phosphatases by a saprophytic fungus to an agar medium was demonstrated. ELF combined with an alkaline buffer led to specific staining of active fungal structures such as hyphae, arbuscules and young vesicles in AM-colonized roots. The results indicate that the ELF substrate is a sensitive indicator for fungal phosphatase activity and that it can be used to visualize hyphal phosphatase activity in both roots and external hyphae. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Mycologia
volume
93
issue
1
pages
17 - 24
publisher
NYBG NEW YORK BOTANICAL GARDEN
external identifiers
  • scopus:0035732933
ISSN
0027-5514
language
English
LU publication?
yes
id
1460814e-5e79-470a-a833-2b5a041f7ad0 (old id 146982)
date added to LUP
2007-07-02 07:45:19
date last changed
2018-05-29 10:38:00
@article{1460814e-5e79-470a-a833-2b5a041f7ad0,
  abstract     = {The location and activity of saprophytic and arbuscular mycorrhizal (NM) fungal phosphatases have been investigated with a fluorogenic substrate (ELF) that forms a fluorescent crystalline precipitate at the site of phosphatase activity. This ELF substrate method was compared with other detection methods such as fast blue RR salt staining and p-nitrophenyl phosphate. The ELF substrate was successfully used to determine the location of phosphatase activity in AM fungal hyphae, and to visualize the active AM fungal colonization of roots. The substrate was taken up by fungal hyphae, when acid and alkaline buffers were used, and internal phosphatase activity was visualized. The release of both acid and alkaline phosphatases by a saprophytic fungus to an agar medium was demonstrated. ELF combined with an alkaline buffer led to specific staining of active fungal structures such as hyphae, arbuscules and young vesicles in AM-colonized roots. The results indicate that the ELF substrate is a sensitive indicator for fungal phosphatase activity and that it can be used to visualize hyphal phosphatase activity in both roots and external hyphae.},
  author       = {van Aarle, Ingrid and Olsson, Pål Axel and Söderström, Bengt},
  issn         = {0027-5514},
  language     = {eng},
  number       = {1},
  pages        = {17--24},
  publisher    = {NYBG NEW YORK BOTANICAL GARDEN},
  series       = {Mycologia},
  title        = {Microscopic detection of phosphatase activity of saprophytic and arbuscular mycorrhizal fungi using a fluorogenic substrate},
  volume       = {93},
  year         = {2001},
}