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Molecular and anatomical evidence for a three-way association between Pinus sylvestris and the ectomycorrhizal fungi Suillus bovinus and Gomphidius roseus

Olsson, Pål Axel LU ; Munzenberger, B; Mahmood, Shahid LU and Erland, Susanne LU (2000) In Mycological Research 104(11). p.1372-1378
Abstract
Many intimate associations between different species of ectomycorrhizal fungi are inferred on the basis of the consistent cooccurrence of their fruit bodies. Suillus bovinus and Gomphidius roseus, where the latter never occurs without the former, is one example. This association was examined with PCR identification and light microscopy. S. bovinus and G. roseus were unambiguously separated on the basis of RFLPs of the PCR-amplified ITS region of ribosomal DNA. Tuberculate mycorrhizas of Pinus sylvestris sampled under fruit bodies of G. roseus and S. bovinus were investigated and the majority were identified as mixed associations involving both G. roseus and S. bovinus. Tuberculate mycorrhizas, which macroscopically resemble the ones of... (More)
Many intimate associations between different species of ectomycorrhizal fungi are inferred on the basis of the consistent cooccurrence of their fruit bodies. Suillus bovinus and Gomphidius roseus, where the latter never occurs without the former, is one example. This association was examined with PCR identification and light microscopy. S. bovinus and G. roseus were unambiguously separated on the basis of RFLPs of the PCR-amplified ITS region of ribosomal DNA. Tuberculate mycorrhizas of Pinus sylvestris sampled under fruit bodies of G. roseus and S. bovinus were investigated and the majority were identified as mixed associations involving both G. roseus and S. bovinus. Tuberculate mycorrhizas, which macroscopically resemble the ones of Suillus species, contained typical chlamydospores of G. roseus and they had haustoria where G. roseus hyphae penetrated the cortical root cells. Pine seedlings collected near the fruit bodies of the two species were mainly colonised by S. bovinus. Mycelial rhizomorphs collected under the fruit bodies of G. roseus were identified as S. bovinus, while both fungal species were present at the base of G. roseus fruit bodies. The significance of these observations and the possibility that G. roseus acts as a parasite are discussed. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Mycological Research
volume
104
issue
11
pages
1372 - 1378
publisher
Cambridge University Press
external identifiers
  • scopus:0034486507
ISSN
1469-8102
DOI
10.1017/S0953756200002823
language
English
LU publication?
yes
id
47967cc8-bca4-4a93-a199-0f93c7b5fe37 (old id 146986)
date added to LUP
2007-06-29 14:11:24
date last changed
2017-01-01 04:58:25
@article{47967cc8-bca4-4a93-a199-0f93c7b5fe37,
  abstract     = {Many intimate associations between different species of ectomycorrhizal fungi are inferred on the basis of the consistent cooccurrence of their fruit bodies. Suillus bovinus and Gomphidius roseus, where the latter never occurs without the former, is one example. This association was examined with PCR identification and light microscopy. S. bovinus and G. roseus were unambiguously separated on the basis of RFLPs of the PCR-amplified ITS region of ribosomal DNA. Tuberculate mycorrhizas of Pinus sylvestris sampled under fruit bodies of G. roseus and S. bovinus were investigated and the majority were identified as mixed associations involving both G. roseus and S. bovinus. Tuberculate mycorrhizas, which macroscopically resemble the ones of Suillus species, contained typical chlamydospores of G. roseus and they had haustoria where G. roseus hyphae penetrated the cortical root cells. Pine seedlings collected near the fruit bodies of the two species were mainly colonised by S. bovinus. Mycelial rhizomorphs collected under the fruit bodies of G. roseus were identified as S. bovinus, while both fungal species were present at the base of G. roseus fruit bodies. The significance of these observations and the possibility that G. roseus acts as a parasite are discussed.},
  author       = {Olsson, Pål Axel and Munzenberger, B and Mahmood, Shahid and Erland, Susanne},
  issn         = {1469-8102},
  language     = {eng},
  number       = {11},
  pages        = {1372--1378},
  publisher    = {Cambridge University Press},
  series       = {Mycological Research},
  title        = {Molecular and anatomical evidence for a three-way association between Pinus sylvestris and the ectomycorrhizal fungi Suillus bovinus and Gomphidius roseus},
  url          = {http://dx.doi.org/10.1017/S0953756200002823},
  volume       = {104},
  year         = {2000},
}