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The effectiveness of enzymatic replacement therapy measured by turbidimetry and the lipaemic index in exocrine pancreatic insufficient young, growing pigs, fed a high-fat diet.

Donaldson, J; Fedkiv, Olexandr LU ; Pawlowska, M; Kowalik, S; Erlwanger, K H; Weström, Björn LU ; Kruszewska, D and Pierzynowski, Stefan LU (2009) In Advances in Medical Sciences2006-01-01+01:00 54(1). p.7-13
Abstract
Purpose: Conventionally, the management of exocrine pancreatic insufficiency (EPI) involves the consumption of a specific diet as well as the replacement of pancreatic enzymes, the effectiveness of which is usually measured by a classical method of blood analyses of non-esterified fatty acids (NEFA) and triglycerides (TG). Dietary supplementation with a pancreatic enzyme preparation (PEP), in conjunction with a high-fat diet, on growth performance, digestibility and absorption (analysed using turbidimetry) of dietary fat in pigs with EPI was investigated.Materials/Methods: EPI was developed by surgical ligation of the pancreatic duct of six male pigs, 6 weeks of age. The pigs were fed a high fat diet (twice daily). A PEP containing 1800 mg... (More)
Purpose: Conventionally, the management of exocrine pancreatic insufficiency (EPI) involves the consumption of a specific diet as well as the replacement of pancreatic enzymes, the effectiveness of which is usually measured by a classical method of blood analyses of non-esterified fatty acids (NEFA) and triglycerides (TG). Dietary supplementation with a pancreatic enzyme preparation (PEP), in conjunction with a high-fat diet, on growth performance, digestibility and absorption (analysed using turbidimetry) of dietary fat in pigs with EPI was investigated.Materials/Methods: EPI was developed by surgical ligation of the pancreatic duct of six male pigs, 6 weeks of age. The pigs were fed a high fat diet (twice daily). A PEP containing 1800 mg entero-coated pancreatin was included in the high fat meals. Blood, urine and faecal samples were collected. The urine and faeces were analysed for dry matter, crude protein and fat content. The lipaemic index and plasma lipid profiles were assessed.Results: EPI completely stopped growth of the pigs. Treatment with PEP significantly increased (P<0.05) growth and body mass as well as the digestibility of dry matter and crude protein. PEP significantly improved the co-efficient of fat absorption, the lipaemic index (measured by turbidimetry methods) and caused significant changes in plasma nonesterified fatty acids and triglyceride concentrations.Conclusions: The short term enzymatic replacement therapy together with a high fat meal has immediate beneficial effects on diet digestibility and on the growth retardation observed in EPI pigs. The turbidimetry method used to measure lipaemic index is a reliable, quick and efficient technique in measuring plasma lipid profiles and thus a good tool for assessing fat absorption. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Advances in Medical Sciences2006-01-01+01:00
volume
54
issue
1
pages
7 - 13
publisher
Versita
external identifiers
  • wos:000279560500002
  • scopus:75349112371
ISSN
1898-4002
DOI
10.2478/v10039-009-0011-x
language
English
LU publication?
yes
id
e6dea265-08dc-47d0-8ab6-e41566e12186 (old id 1470170)
date added to LUP
2009-09-08 13:49:19
date last changed
2017-08-13 03:45:05
@article{e6dea265-08dc-47d0-8ab6-e41566e12186,
  abstract     = {Purpose: Conventionally, the management of exocrine pancreatic insufficiency (EPI) involves the consumption of a specific diet as well as the replacement of pancreatic enzymes, the effectiveness of which is usually measured by a classical method of blood analyses of non-esterified fatty acids (NEFA) and triglycerides (TG). Dietary supplementation with a pancreatic enzyme preparation (PEP), in conjunction with a high-fat diet, on growth performance, digestibility and absorption (analysed using turbidimetry) of dietary fat in pigs with EPI was investigated.Materials/Methods: EPI was developed by surgical ligation of the pancreatic duct of six male pigs, 6 weeks of age. The pigs were fed a high fat diet (twice daily). A PEP containing 1800 mg entero-coated pancreatin was included in the high fat meals. Blood, urine and faecal samples were collected. The urine and faeces were analysed for dry matter, crude protein and fat content. The lipaemic index and plasma lipid profiles were assessed.Results: EPI completely stopped growth of the pigs. Treatment with PEP significantly increased (P&lt;0.05) growth and body mass as well as the digestibility of dry matter and crude protein. PEP significantly improved the co-efficient of fat absorption, the lipaemic index (measured by turbidimetry methods) and caused significant changes in plasma nonesterified fatty acids and triglyceride concentrations.Conclusions: The short term enzymatic replacement therapy together with a high fat meal has immediate beneficial effects on diet digestibility and on the growth retardation observed in EPI pigs. The turbidimetry method used to measure lipaemic index is a reliable, quick and efficient technique in measuring plasma lipid profiles and thus a good tool for assessing fat absorption.},
  author       = {Donaldson, J and Fedkiv, Olexandr and Pawlowska, M and Kowalik, S and Erlwanger, K H and Weström, Björn and Kruszewska, D and Pierzynowski, Stefan},
  issn         = {1898-4002},
  language     = {eng},
  number       = {1},
  pages        = {7--13},
  publisher    = {Versita},
  series       = {Advances in Medical Sciences2006-01-01+01:00},
  title        = {The effectiveness of enzymatic replacement therapy measured by turbidimetry and the lipaemic index in exocrine pancreatic insufficient young, growing pigs, fed a high-fat diet.},
  url          = {http://dx.doi.org/10.2478/v10039-009-0011-x},
  volume       = {54},
  year         = {2009},
}