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LY294002 Enhances Boswellic Acid-induced Apoptosis in Colon Cancer Cells

Liu, Jian-Jun and Duan, Rui-Dong LU (2009) In Anticancer research 29(8). p.2987-2991
Abstract
Background: Boswellic acids, a type of pentacyclic triterpenoids, have been shown to induce apoptosis in colon cancer cells. The phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt) pathway is crucial for cell proliferation and survival. Whether the apoptotic effects of boswellic acid could be affected by inhibition of PI3K/Akt pathway was examined. Materials and Methods: Colon cancer HT29 cells were treated with 3-acetyl-11-keto-beta boswellic acid AKBA in the absence and presence of LY294002 or Wortmanin, inhibitors of PI3K. Apoptosis was determined by flow cytometry and caspase assay. The activation of Akt was examined by Western blot. Results: AKBA at 30 mu M only slightly induced apoptosis. Preincubation of the cells with... (More)
Background: Boswellic acids, a type of pentacyclic triterpenoids, have been shown to induce apoptosis in colon cancer cells. The phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt) pathway is crucial for cell proliferation and survival. Whether the apoptotic effects of boswellic acid could be affected by inhibition of PI3K/Akt pathway was examined. Materials and Methods: Colon cancer HT29 cells were treated with 3-acetyl-11-keto-beta boswellic acid AKBA in the absence and presence of LY294002 or Wortmanin, inhibitors of PI3K. Apoptosis was determined by flow cytometry and caspase assay. The activation of Akt was examined by Western blot. Results: AKBA at 30 mu M only slightly induced apoptosis. Preincubation of the cells with LY294002 or wortmannin significantly enhanced the AKBA-induced apoptosis up to 20-fold. Further study showed that at the doses used, AKBA induced phosphorylation of Akt at both Ser473 and Thr308 positions, indicating an activation of the PI3K/Akt pathway. Conclusion: AKBA may activate the PI3K/Akt pathway and inhibition of the P13K pathway significantly enhances AKBA-induced apoptosis. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
colon cancer cells, Akt, PI3 kinase, Apoptosis, boswellic acids, LY294002, wortmannin
in
Anticancer research
volume
29
issue
8
pages
2987 - 2991
publisher
International Institute of Cancer Research
external identifiers
  • wos:000268846800016
  • scopus:68549111000
ISSN
1791-7530
language
English
LU publication?
yes
id
264482bd-167f-4516-9f90-71fe4b2336ba (old id 1478432)
date added to LUP
2016-04-01 12:22:02
date last changed
2024-10-09 07:32:42
@article{264482bd-167f-4516-9f90-71fe4b2336ba,
  abstract     = {{Background: Boswellic acids, a type of pentacyclic triterpenoids, have been shown to induce apoptosis in colon cancer cells. The phosphatidylinositol-3-kinase (PI3K)/protein kinase B (Akt) pathway is crucial for cell proliferation and survival. Whether the apoptotic effects of boswellic acid could be affected by inhibition of PI3K/Akt pathway was examined. Materials and Methods: Colon cancer HT29 cells were treated with 3-acetyl-11-keto-beta boswellic acid AKBA in the absence and presence of LY294002 or Wortmanin, inhibitors of PI3K. Apoptosis was determined by flow cytometry and caspase assay. The activation of Akt was examined by Western blot. Results: AKBA at 30 mu M only slightly induced apoptosis. Preincubation of the cells with LY294002 or wortmannin significantly enhanced the AKBA-induced apoptosis up to 20-fold. Further study showed that at the doses used, AKBA induced phosphorylation of Akt at both Ser473 and Thr308 positions, indicating an activation of the PI3K/Akt pathway. Conclusion: AKBA may activate the PI3K/Akt pathway and inhibition of the P13K pathway significantly enhances AKBA-induced apoptosis.}},
  author       = {{Liu, Jian-Jun and Duan, Rui-Dong}},
  issn         = {{1791-7530}},
  keywords     = {{colon cancer cells; Akt; PI3 kinase; Apoptosis; boswellic acids; LY294002; wortmannin}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{2987--2991}},
  publisher    = {{International Institute of Cancer Research}},
  series       = {{Anticancer research}},
  title        = {{LY294002 Enhances Boswellic Acid-induced Apoptosis in Colon Cancer Cells}},
  volume       = {{29}},
  year         = {{2009}},
}