Cytological and molecular description of Hamiltosporidium tvaerminnensis gen. et sp nov., a microsporidian parasite of Daphnia magna, and establishment of Hamiltosporidium magnivora comb. nov.
(2011) In Parasitology 138(4). p.447-462- Abstract
- We describe the new microsporidium Hamiltosporidium tvaerminnensis gen. et sp. nov. with an emphasis on its ultrastructural characteristics and phylogenetic position as inferred from the sequence data of SSU rDNA, alpha-and beta-tubulin. This parasite was previously identified as Octosporea bayeri Jirovec, 1936 and has become a model system to study the ecology, epidemiology, evolution and genomics of microsporidia - host interactions. Here, we present evidence that shows its differences from O. bayeri. Hamiltosporidium tvaerminnensis exclusively infects the adipose tissue, the ovaries and the hypodermis of Daphnia magna and is found only in host populations located in coastal rock pool populations in Finland and Sweden. Merogonial stages... (More)
- We describe the new microsporidium Hamiltosporidium tvaerminnensis gen. et sp. nov. with an emphasis on its ultrastructural characteristics and phylogenetic position as inferred from the sequence data of SSU rDNA, alpha-and beta-tubulin. This parasite was previously identified as Octosporea bayeri Jirovec, 1936 and has become a model system to study the ecology, epidemiology, evolution and genomics of microsporidia - host interactions. Here, we present evidence that shows its differences from O. bayeri. Hamiltosporidium tvaerminnensis exclusively infects the adipose tissue, the ovaries and the hypodermis of Daphnia magna and is found only in host populations located in coastal rock pool populations in Finland and Sweden. Merogonial stages of H. tvaerminnensis have isolated nuclei; merozoites are formed by binary fission or by the cleaving of a plasmodium with a small number of nuclei. A sporogonial plasmodium with isolated nuclei yields 8 sporoblasts. Elongated spores are generated by the most finger-like plasmodia. The mature spores are polymorphic in shape and size. Most spores are pyriform (4.9-5.6x2.2-2.3 mu m) and have their polar filament arranged in 12-13 coils. A second, elongated spore type (6.8-12.0x1.6-2.1 mu m) is rod-shaped with blunt ends and measures 6.8-12.0x1.6-2.1 mu m. The envelope of the sporophorous vesicle is thin and fragile, formed at the beginning of the sporogony. Cytological and molecular comparisons with Flabelliforma magnivora, a parasite infecting the same tissues in the same host species, reveal that these two species are very closely related, yet distinct. Moreover, both cytological and molecular data indicate that these species are quite distant from F. montana, the type species of the genus Flabelliforma. We therefore propose that F. magnivora also be placed in Hamiltosporidium gen. nov. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1925763
- author
- Haag, Karen Luisa ; Larsson, Ronny LU ; Refardt, Dominik and Ebert, Dieter
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Ultrastructural characteristics, molecular phylogeny, Octosporea, bayeri, Flabelliforma magnivora, Microsporidia, Cladocerca
- in
- Parasitology
- volume
- 138
- issue
- 4
- pages
- 447 - 462
- publisher
- Cambridge University Press
- external identifiers
-
- wos:000288614600004
- scopus:79957477610
- pmid:20946698
- ISSN
- 1469-8161
- DOI
- 10.1017/S0031182010001393
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Zoology (Closed 2011) (011012000)
- id
- 1481f421-e005-4234-aaf9-79a7fea47429 (old id 1925763)
- date added to LUP
- 2016-04-01 10:11:34
- date last changed
- 2022-04-12 02:50:46
@article{1481f421-e005-4234-aaf9-79a7fea47429,
abstract = {{We describe the new microsporidium Hamiltosporidium tvaerminnensis gen. et sp. nov. with an emphasis on its ultrastructural characteristics and phylogenetic position as inferred from the sequence data of SSU rDNA, alpha-and beta-tubulin. This parasite was previously identified as Octosporea bayeri Jirovec, 1936 and has become a model system to study the ecology, epidemiology, evolution and genomics of microsporidia - host interactions. Here, we present evidence that shows its differences from O. bayeri. Hamiltosporidium tvaerminnensis exclusively infects the adipose tissue, the ovaries and the hypodermis of Daphnia magna and is found only in host populations located in coastal rock pool populations in Finland and Sweden. Merogonial stages of H. tvaerminnensis have isolated nuclei; merozoites are formed by binary fission or by the cleaving of a plasmodium with a small number of nuclei. A sporogonial plasmodium with isolated nuclei yields 8 sporoblasts. Elongated spores are generated by the most finger-like plasmodia. The mature spores are polymorphic in shape and size. Most spores are pyriform (4.9-5.6x2.2-2.3 mu m) and have their polar filament arranged in 12-13 coils. A second, elongated spore type (6.8-12.0x1.6-2.1 mu m) is rod-shaped with blunt ends and measures 6.8-12.0x1.6-2.1 mu m. The envelope of the sporophorous vesicle is thin and fragile, formed at the beginning of the sporogony. Cytological and molecular comparisons with Flabelliforma magnivora, a parasite infecting the same tissues in the same host species, reveal that these two species are very closely related, yet distinct. Moreover, both cytological and molecular data indicate that these species are quite distant from F. montana, the type species of the genus Flabelliforma. We therefore propose that F. magnivora also be placed in Hamiltosporidium gen. nov.}},
author = {{Haag, Karen Luisa and Larsson, Ronny and Refardt, Dominik and Ebert, Dieter}},
issn = {{1469-8161}},
keywords = {{Ultrastructural characteristics; molecular phylogeny; Octosporea; bayeri; Flabelliforma magnivora; Microsporidia; Cladocerca}},
language = {{eng}},
number = {{4}},
pages = {{447--462}},
publisher = {{Cambridge University Press}},
series = {{Parasitology}},
title = {{Cytological and molecular description of Hamiltosporidium tvaerminnensis gen. et sp nov., a microsporidian parasite of Daphnia magna, and establishment of Hamiltosporidium magnivora comb. nov.}},
url = {{http://dx.doi.org/10.1017/S0031182010001393}},
doi = {{10.1017/S0031182010001393}},
volume = {{138}},
year = {{2011}},
}