Differential proteome analysis of the preeclamptic placenta using optimized protein extraction.
Centlow, Magnus LU ; Hansson, Stefan LU
and Welinder, Charlotte
LU
(2010)
In Journal of Biomedicine and Biotechnology
2010(Sep 13).
- Abstract
- The human placenta is a difficult tissue to work with using proteomic technology since it contains large amounts of lipids and glycogen. Both lipids and glycogen are known to interfere with the first step in the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the isoelectric focusing. In order to gain the best possible protein separation on 2D-PAGE, an optimized sample preparation protocol for placental proteins was developed. Two different buffers, urea/CHAPS and Hepes, were used for solubilization in combination with six different precipitation methods. The removal of glycogen from the samples by centrifugation was crucial for the final proteome maps. Solubilization with urea/CHAPS in combination with... (More)
- The human placenta is a difficult tissue to work with using proteomic technology since it contains large amounts of lipids and glycogen. Both lipids and glycogen are known to interfere with the first step in the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the isoelectric focusing. In order to gain the best possible protein separation on 2D-PAGE, an optimized sample preparation protocol for placental proteins was developed. Two different buffers, urea/CHAPS and Hepes, were used for solubilization in combination with six different precipitation methods. The removal of glycogen from the samples by centrifugation was crucial for the final proteome maps. Solubilization with urea/CHAPS in combination with dichloromethane/methanol or acidified acetone proved to be the best precipitation procedures. When applied to clinical placenta samples apolipoprotein A1 was found to be accumulated in the preeclamptic placenta, where it may either have a nutritional effect or act as a modifier of signal transduction. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1483439
- author
- Centlow, Magnus
LU
; Hansson, Stefan
LU
and Welinder, Charlotte
LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biomedicine and Biotechnology
- volume
- 2010
- issue
- Sep 13
- article number
- 458748
- publisher
- Hindawi Limited
- external identifiers
-
- wos:000271075100001
- pmid:19756160
- scopus:70449715729
- pmid:19756160
- ISSN
- 1110-7251
- DOI
- 10.1155/2010/458748
- language
- English
- LU publication?
- yes
- id
- 29d67e30-a75c-4fa2-ac36-101a1b7e6068 (old id 1483439)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/19756160?dopt=Abstract
- date added to LUP
- 2016-04-04 07:00:09
- date last changed
- 2022-01-29 01:33:25
@article{29d67e30-a75c-4fa2-ac36-101a1b7e6068,
abstract = {{The human placenta is a difficult tissue to work with using proteomic technology since it contains large amounts of lipids and glycogen. Both lipids and glycogen are known to interfere with the first step in the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), the isoelectric focusing. In order to gain the best possible protein separation on 2D-PAGE, an optimized sample preparation protocol for placental proteins was developed. Two different buffers, urea/CHAPS and Hepes, were used for solubilization in combination with six different precipitation methods. The removal of glycogen from the samples by centrifugation was crucial for the final proteome maps. Solubilization with urea/CHAPS in combination with dichloromethane/methanol or acidified acetone proved to be the best precipitation procedures. When applied to clinical placenta samples apolipoprotein A1 was found to be accumulated in the preeclamptic placenta, where it may either have a nutritional effect or act as a modifier of signal transduction.}},
author = {{Centlow, Magnus and Hansson, Stefan and Welinder, Charlotte}},
issn = {{1110-7251}},
language = {{eng}},
number = {{Sep 13}},
publisher = {{Hindawi Limited}},
series = {{Journal of Biomedicine and Biotechnology}},
title = {{Differential proteome analysis of the preeclamptic placenta using optimized protein extraction.}},
url = {{http://dx.doi.org/10.1155/2010/458748}},
doi = {{10.1155/2010/458748}},
volume = {{2010}},
year = {{2010}},
}