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Complex Nature of the Genome in a Wine-spoilage Yeast, Dekkera bruxellensis.

Hellborg, Linda LU and Piskur, Jure LU (2009) In Eukaryotic Cell 8(11). p.1739-1749
Abstract
When the genome organization of thirty native isolates belonging to a wine-spoilage yeast Dekkera/Brettanomyces bruxellensis, a distant relative of Saccharomyces cerevisiae, was examined, the number of chromosomes varied drastically, from 4 to at least 9. When single gene probes were used in Southern analysis, the corresponding genes usually mapped to at least two chromosomal bands, excluding a simple haploid organization of the genome. When different loci were sequenced, in most cases several different haplotypes were obtained in each single isolate, and they belonged to two sub-types. Phylogenetic reconstruction using haplotypes revealed that the sequences from different isolates, belonging to one sub-type, were more similar to each... (More)
When the genome organization of thirty native isolates belonging to a wine-spoilage yeast Dekkera/Brettanomyces bruxellensis, a distant relative of Saccharomyces cerevisiae, was examined, the number of chromosomes varied drastically, from 4 to at least 9. When single gene probes were used in Southern analysis, the corresponding genes usually mapped to at least two chromosomal bands, excluding a simple haploid organization of the genome. When different loci were sequenced, in most cases several different haplotypes were obtained in each single isolate, and they belonged to two sub-types. Phylogenetic reconstruction using haplotypes revealed that the sequences from different isolates, belonging to one sub-type, were more similar to each other than to the sequences belonging to the other sub-type, within the isolate. Reanalysis of the genome sequence confirmed that also the partially sequenced strain Y879 is not a simple hybrid and its genome contains of app. 1% polymorphic sites. The present situation could be explained by (i) a hybridization event where two similar but different genomes have recently fused together or, (ii) that the diploid progenitor of all analyzed strains lost a regular sexual cycle and the genome started to accumulate mutations. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Eukaryotic Cell
volume
8
issue
11
pages
1739 - 1749
publisher
American Society for Microbiology
external identifiers
  • wos:000271368700012
  • scopus:72449150687
ISSN
1535-9778
DOI
10.1128/EC.00115-09
language
English
LU publication?
yes
id
3dbff1d5-9319-4199-882e-fb3bf7601bb7 (old id 1483999)
date added to LUP
2009-10-08 12:13:32
date last changed
2017-10-22 03:40:35
@article{3dbff1d5-9319-4199-882e-fb3bf7601bb7,
  abstract     = {When the genome organization of thirty native isolates belonging to a wine-spoilage yeast Dekkera/Brettanomyces bruxellensis, a distant relative of Saccharomyces cerevisiae, was examined, the number of chromosomes varied drastically, from 4 to at least 9. When single gene probes were used in Southern analysis, the corresponding genes usually mapped to at least two chromosomal bands, excluding a simple haploid organization of the genome. When different loci were sequenced, in most cases several different haplotypes were obtained in each single isolate, and they belonged to two sub-types. Phylogenetic reconstruction using haplotypes revealed that the sequences from different isolates, belonging to one sub-type, were more similar to each other than to the sequences belonging to the other sub-type, within the isolate. Reanalysis of the genome sequence confirmed that also the partially sequenced strain Y879 is not a simple hybrid and its genome contains of app. 1% polymorphic sites. The present situation could be explained by (i) a hybridization event where two similar but different genomes have recently fused together or, (ii) that the diploid progenitor of all analyzed strains lost a regular sexual cycle and the genome started to accumulate mutations.},
  author       = {Hellborg, Linda and Piskur, Jure},
  issn         = {1535-9778},
  language     = {eng},
  number       = {11},
  pages        = {1739--1749},
  publisher    = {American Society for Microbiology},
  series       = {Eukaryotic Cell},
  title        = {Complex Nature of the Genome in a Wine-spoilage Yeast, Dekkera bruxellensis.},
  url          = {http://dx.doi.org/10.1128/EC.00115-09},
  volume       = {8},
  year         = {2009},
}