Complex Nature of the Genome in a Wine-spoilage Yeast, Dekkera bruxellensis.
(2009) In Eukaryotic Cell 8(11). p.1739-1749- Abstract
- When the genome organization of thirty native isolates belonging to a wine-spoilage yeast Dekkera/Brettanomyces bruxellensis, a distant relative of Saccharomyces cerevisiae, was examined, the number of chromosomes varied drastically, from 4 to at least 9. When single gene probes were used in Southern analysis, the corresponding genes usually mapped to at least two chromosomal bands, excluding a simple haploid organization of the genome. When different loci were sequenced, in most cases several different haplotypes were obtained in each single isolate, and they belonged to two sub-types. Phylogenetic reconstruction using haplotypes revealed that the sequences from different isolates, belonging to one sub-type, were more similar to each... (More)
- When the genome organization of thirty native isolates belonging to a wine-spoilage yeast Dekkera/Brettanomyces bruxellensis, a distant relative of Saccharomyces cerevisiae, was examined, the number of chromosomes varied drastically, from 4 to at least 9. When single gene probes were used in Southern analysis, the corresponding genes usually mapped to at least two chromosomal bands, excluding a simple haploid organization of the genome. When different loci were sequenced, in most cases several different haplotypes were obtained in each single isolate, and they belonged to two sub-types. Phylogenetic reconstruction using haplotypes revealed that the sequences from different isolates, belonging to one sub-type, were more similar to each other than to the sequences belonging to the other sub-type, within the isolate. Reanalysis of the genome sequence confirmed that also the partially sequenced strain Y879 is not a simple hybrid and its genome contains of app. 1% polymorphic sites. The present situation could be explained by (i) a hybridization event where two similar but different genomes have recently fused together or, (ii) that the diploid progenitor of all analyzed strains lost a regular sexual cycle and the genome started to accumulate mutations. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1483999
- author
- Hellborg, Linda LU and Piskur, Jure LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Eukaryotic Cell
- volume
- 8
- issue
- 11
- pages
- 1739 - 1749
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000271368700012
- scopus:72449150687
- ISSN
- 1535-9778
- DOI
- 10.1128/EC.00115-09
- language
- English
- LU publication?
- yes
- id
- 3dbff1d5-9319-4199-882e-fb3bf7601bb7 (old id 1483999)
- date added to LUP
- 2016-04-01 11:53:36
- date last changed
- 2022-04-28 21:34:02
@article{3dbff1d5-9319-4199-882e-fb3bf7601bb7,
abstract = {{When the genome organization of thirty native isolates belonging to a wine-spoilage yeast Dekkera/Brettanomyces bruxellensis, a distant relative of Saccharomyces cerevisiae, was examined, the number of chromosomes varied drastically, from 4 to at least 9. When single gene probes were used in Southern analysis, the corresponding genes usually mapped to at least two chromosomal bands, excluding a simple haploid organization of the genome. When different loci were sequenced, in most cases several different haplotypes were obtained in each single isolate, and they belonged to two sub-types. Phylogenetic reconstruction using haplotypes revealed that the sequences from different isolates, belonging to one sub-type, were more similar to each other than to the sequences belonging to the other sub-type, within the isolate. Reanalysis of the genome sequence confirmed that also the partially sequenced strain Y879 is not a simple hybrid and its genome contains of app. 1% polymorphic sites. The present situation could be explained by (i) a hybridization event where two similar but different genomes have recently fused together or, (ii) that the diploid progenitor of all analyzed strains lost a regular sexual cycle and the genome started to accumulate mutations.}},
author = {{Hellborg, Linda and Piskur, Jure}},
issn = {{1535-9778}},
language = {{eng}},
number = {{11}},
pages = {{1739--1749}},
publisher = {{American Society for Microbiology}},
series = {{Eukaryotic Cell}},
title = {{Complex Nature of the Genome in a Wine-spoilage Yeast, Dekkera bruxellensis.}},
url = {{http://dx.doi.org/10.1128/EC.00115-09}},
doi = {{10.1128/EC.00115-09}},
volume = {{8}},
year = {{2009}},
}