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Incorporation of antimicrobial compounds in mesoporous silica film monolith

Izquierdo-Barba, Isabel; Vallet-Regi, Maria; Kupferschmidt, Natalia; Terasaki, Osamu; Schmidtchen, Artur LU and Malmsten, Martin (2009) In Biomaterials 30(29). p.5729-5736
Abstract
Incorporation of the antimicrobial peptide LL-37 (LLGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES), as well as low molecular weight antimicrobial chlorhexidine, into mesoporous silica was obtained using an EISA one-pot synthesis method. FTIR confirmed efficient encapsulation of both LL-37 and chlorhexidine into mesoporous silica, while XRD and TEM showed that antimicrobial agent incorporation can be achieved without greatly affecting the structure of the mesoporous silica. The modified mesoporous silica released LL-37 and chlorhexidine slowly, reaching maximum release after about 200 h. The release rate could also be controlled through incorporation of SH groups in the pore walls, adding to pore hydrophobicity and reducing the release rate by about... (More)
Incorporation of the antimicrobial peptide LL-37 (LLGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES), as well as low molecular weight antimicrobial chlorhexidine, into mesoporous silica was obtained using an EISA one-pot synthesis method. FTIR confirmed efficient encapsulation of both LL-37 and chlorhexidine into mesoporous silica, while XRD and TEM showed that antimicrobial agent incorporation can be achieved without greatly affecting the structure of the mesoporous silica. The modified mesoporous silica released LL-37 and chlorhexidine slowly, reaching maximum release after about 200 h. The release rate could also be controlled through incorporation of SH groups in the pore walls, adding to pore hydrophobicity and reducing the release rate by about 50% compared to the unmodified mesoporous silica. Mesoporous silica containing either LL-37 or chlorhexidine displayed potent bactericidal properties against both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. While chlorhexidine-loaded mesoporous silica displayed an accompanying high toxicity, as judged from hemolysis, LDH release, and MTT assay, the corresponding material containing LL-37 showed very low toxicity by all these assays, comparable to that observed for mesoporous silica in the absence of antibacterial drug, as well as to the negative controls in the respective assays. Mesoporous silica containing LL-37 therefore holds potential as an implantable material or a surface coating for such materials, as it combines potent bactericidal action with low toxicity, important features for controlling implant-related infections, e.g., for multiresistant pathogens or for cases where access to the infection site of systemically administered antibiotics is limited due to collagen capsule formation or other factors. (C) 2009 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Silica, Mesoporous, LL-37, Antimicrobial, Chlorhexicline
in
Biomaterials
volume
30
issue
29
pages
5729 - 5736
publisher
Elsevier
external identifiers
  • wos:000270115200053
  • scopus:68549110160
ISSN
1878-5905
DOI
10.1016/j.biomaterials.2009.07.003
language
English
LU publication?
yes
id
8f92fc70-222c-4c40-8452-66e36661e1f2 (old id 1490142)
date added to LUP
2009-10-19 15:45:00
date last changed
2017-12-10 03:40:04
@article{8f92fc70-222c-4c40-8452-66e36661e1f2,
  abstract     = {Incorporation of the antimicrobial peptide LL-37 (LLGDFFRKSKEKIGKEFKRIVQRIKDFLRNLVPRTES), as well as low molecular weight antimicrobial chlorhexidine, into mesoporous silica was obtained using an EISA one-pot synthesis method. FTIR confirmed efficient encapsulation of both LL-37 and chlorhexidine into mesoporous silica, while XRD and TEM showed that antimicrobial agent incorporation can be achieved without greatly affecting the structure of the mesoporous silica. The modified mesoporous silica released LL-37 and chlorhexidine slowly, reaching maximum release after about 200 h. The release rate could also be controlled through incorporation of SH groups in the pore walls, adding to pore hydrophobicity and reducing the release rate by about 50% compared to the unmodified mesoporous silica. Mesoporous silica containing either LL-37 or chlorhexidine displayed potent bactericidal properties against both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. While chlorhexidine-loaded mesoporous silica displayed an accompanying high toxicity, as judged from hemolysis, LDH release, and MTT assay, the corresponding material containing LL-37 showed very low toxicity by all these assays, comparable to that observed for mesoporous silica in the absence of antibacterial drug, as well as to the negative controls in the respective assays. Mesoporous silica containing LL-37 therefore holds potential as an implantable material or a surface coating for such materials, as it combines potent bactericidal action with low toxicity, important features for controlling implant-related infections, e.g., for multiresistant pathogens or for cases where access to the infection site of systemically administered antibiotics is limited due to collagen capsule formation or other factors. (C) 2009 Elsevier Ltd. All rights reserved.},
  author       = {Izquierdo-Barba, Isabel and Vallet-Regi, Maria and Kupferschmidt, Natalia and Terasaki, Osamu and Schmidtchen, Artur and Malmsten, Martin},
  issn         = {1878-5905},
  keyword      = {Silica,Mesoporous,LL-37,Antimicrobial,Chlorhexicline},
  language     = {eng},
  number       = {29},
  pages        = {5729--5736},
  publisher    = {Elsevier},
  series       = {Biomaterials},
  title        = {Incorporation of antimicrobial compounds in mesoporous silica film monolith},
  url          = {http://dx.doi.org/10.1016/j.biomaterials.2009.07.003},
  volume       = {30},
  year         = {2009},
}