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Spatial and Temporal Distribution of Sucrose Synthase in the Radish Hypocotyl in Relation to Thickening Growth

Rouhier, Hervé LU and Usuda, H (2001) In Plant and Cell Physiology 42(6). p.583-593
Abstract
Sucrose synthase (SuSy) is a key enzyme in the development of storage root of radish. Clarification of its spatial and temporal expression during the thickening growth of radish hypocotyl, which later develops into storage root, was carried out immunologically using light microscopy. Sequential harvests at 3, 7, 11 and 13 d after sowing (DAS) were performed on two radish cultivars having different sink capacity. A very low level of SuSy was observed 3 DAS for both cultivars. White Cherrish (WC; strong storage root) showed the maximum level of SuSy between 7 and 11 DAS with increased cell development (thickening), while in Kosena (K; low storage root) the level remained high after 13 d of growth. A high level of SuSy was found in companion... (More)
Sucrose synthase (SuSy) is a key enzyme in the development of storage root of radish. Clarification of its spatial and temporal expression during the thickening growth of radish hypocotyl, which later develops into storage root, was carried out immunologically using light microscopy. Sequential harvests at 3, 7, 11 and 13 d after sowing (DAS) were performed on two radish cultivars having different sink capacity. A very low level of SuSy was observed 3 DAS for both cultivars. White Cherrish (WC; strong storage root) showed the maximum level of SuSy between 7 and 11 DAS with increased cell development (thickening), while in Kosena (K; low storage root) the level remained high after 13 d of growth. A high level of SuSy was found in companion cells, which was consistent with previous observations, but SuSy was also found in the xylem parenchyma and in some cortical cells. The level of SuSy differed according to the localization and depended highly on cell development. Both cell division and cell enlargement were stimulated in WC compared with K. The role of SuSy during thickening growth of radish hypocotyl is discussed in terms of utilizing photosynthates. (Less)
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Contribution to journal
publication status
published
subject
in
Plant and Cell Physiology
volume
42
issue
6
pages
583 - 593
publisher
Oxford University Press
external identifiers
  • scopus:0034953548
ISSN
1471-9053
language
English
LU publication?
yes
id
4a061b90-b1ae-4144-92f5-c5ff5da861f1 (old id 149417)
alternative location
http://pcp.oxfordjournals.org/cgi/content/full/42/6/583
date added to LUP
2007-06-29 14:35:20
date last changed
2018-05-29 09:58:32
@article{4a061b90-b1ae-4144-92f5-c5ff5da861f1,
  abstract     = {Sucrose synthase (SuSy) is a key enzyme in the development of storage root of radish. Clarification of its spatial and temporal expression during the thickening growth of radish hypocotyl, which later develops into storage root, was carried out immunologically using light microscopy. Sequential harvests at 3, 7, 11 and 13 d after sowing (DAS) were performed on two radish cultivars having different sink capacity. A very low level of SuSy was observed 3 DAS for both cultivars. White Cherrish (WC; strong storage root) showed the maximum level of SuSy between 7 and 11 DAS with increased cell development (thickening), while in Kosena (K; low storage root) the level remained high after 13 d of growth. A high level of SuSy was found in companion cells, which was consistent with previous observations, but SuSy was also found in the xylem parenchyma and in some cortical cells. The level of SuSy differed according to the localization and depended highly on cell development. Both cell division and cell enlargement were stimulated in WC compared with K. The role of SuSy during thickening growth of radish hypocotyl is discussed in terms of utilizing photosynthates.},
  author       = {Rouhier, Hervé and Usuda, H},
  issn         = {1471-9053},
  language     = {eng},
  number       = {6},
  pages        = {583--593},
  publisher    = {Oxford University Press},
  series       = {Plant and Cell Physiology},
  title        = {Spatial and Temporal Distribution of Sucrose Synthase in the Radish Hypocotyl in Relation to Thickening Growth},
  volume       = {42},
  year         = {2001},
}