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Increased expression of the oxidative pentose phosphate pathway and gluconeogenesis in anaerobically growing xylose-utilizing Saccharomyces cerevisiae

Runquist, David LU ; Hahn-Hägerdal, Bärbel LU and Bettiga, Maurizio LU (2009) In Microbial Cell Factories 8(1).
Abstract
Fermentation of xylose to ethanol has been achieved in S. cerevisiae by genetic engineering. Xylose utilization is however slow compared to glucose, and during anaerobic conditions addition of glucose has been necessary for cellular growth. In the current study, the xylose-utilizing strain TMB 3415 was employed to investigate differences between anaerobic utilization of glucose and xylose. This strain carried a xylose reductase (XYL1 K270R) engineered for increased NADH utilization and was capable of sustained anaerobic growth on xylose as sole carbon source. Metabolic and transcriptional characterization could thus for the first time be performed without addition of a co-substrate or oxygen.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Microbial Cell Factories
volume
8
issue
1
publisher
BioMed Central
external identifiers
  • wos:000270970500001
  • scopus:70449428931
ISSN
1475-2859
DOI
10.1186/1475-2859-8-49
language
English
LU publication?
yes
id
927d2729-1d03-4d9a-a7fc-c3b9bb777544 (old id 1497411)
date added to LUP
2009-11-25 12:33:54
date last changed
2017-12-10 03:59:15
@article{927d2729-1d03-4d9a-a7fc-c3b9bb777544,
  abstract     = {Fermentation of xylose to ethanol has been achieved in S. cerevisiae by genetic engineering. Xylose utilization is however slow compared to glucose, and during anaerobic conditions addition of glucose has been necessary for cellular growth. In the current study, the xylose-utilizing strain TMB 3415 was employed to investigate differences between anaerobic utilization of glucose and xylose. This strain carried a xylose reductase (XYL1 K270R) engineered for increased NADH utilization and was capable of sustained anaerobic growth on xylose as sole carbon source. Metabolic and transcriptional characterization could thus for the first time be performed without addition of a co-substrate or oxygen.},
  articleno    = {49},
  author       = {Runquist, David and Hahn-Hägerdal, Bärbel and Bettiga, Maurizio},
  issn         = {1475-2859},
  language     = {eng},
  number       = {1},
  publisher    = {BioMed Central},
  series       = {Microbial Cell Factories},
  title        = {Increased expression of the oxidative pentose phosphate pathway and gluconeogenesis in anaerobically growing xylose-utilizing Saccharomyces cerevisiae},
  url          = {http://dx.doi.org/10.1186/1475-2859-8-49},
  volume       = {8},
  year         = {2009},
}