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Characterization of Hydropsyche slossonae (Trichoptera : Hydropsychidae) capture net polypeptides

Tessier, L; Biosvert, J L; Vought, Lena LU and Lacoursière, Jean LU (2000) In Canadian Entomologist 132(1). p.59-68
Abstract
The aim of this study was to characterize polypeptide components of the capture net spun by trichopteran larvae Hydropsyche slossonae (Banks) (Trichoptera: Hydropsychidae). Thirty-one polypeptide bands were identified by SDS - polyacrylamide gel electrophoresis (SDS-PAGE) from extracted net material, with molecular weights ranging from 8500 to 179 000. Comparison with published data on Bombyx mori (L.) (Lepidoptera: Bombycidae) silk, treated under similar denaturing conditions, shows that six low molecular weight polypeptides ranging between 8500 and 18 800 in the silk of H. slossonae are absent from that of B. mori; furthermore, two high molecular weight polypeptides (210 000 and 220 000) detected in the silk of B. mori are not present in... (More)
The aim of this study was to characterize polypeptide components of the capture net spun by trichopteran larvae Hydropsyche slossonae (Banks) (Trichoptera: Hydropsychidae). Thirty-one polypeptide bands were identified by SDS - polyacrylamide gel electrophoresis (SDS-PAGE) from extracted net material, with molecular weights ranging from 8500 to 179 000. Comparison with published data on Bombyx mori (L.) (Lepidoptera: Bombycidae) silk, treated under similar denaturing conditions, shows that six low molecular weight polypeptides ranging between 8500 and 18 800 in the silk of H. slossonae are absent from that of B. mori; furthermore, two high molecular weight polypeptides (210 000 and 220 000) detected in the silk of B. mori are not present in that of H. slossonae. Differences between both groups are probably related to their mode of living and to the specific use of silk (in air versus under water). Our findings are consistent with the current trend in the literature that silk spun by aquatic and terrestrial insects, as well as those spun by different species, is apparently made of different biopolymers according to the protein constituents. Hence, the polypeptide characterization of silk, combined with sequence data and (or) antibodies cross-reactivity data, could represent a potential tool for taxonomic classification improvement of aquatic insects. These results could eventually be used to characterize hydropsychid capture net anomalies induced by environmental pollution. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Canadian Entomologist
volume
132
issue
1
pages
59 - 68
publisher
Entomological Society of Canada
external identifiers
  • scopus:0034030059
ISSN
1918-3240
language
English
LU publication?
yes
id
6afd16b1-dd05-41ca-af64-204643588c02 (old id 149818)
date added to LUP
2007-06-29 08:12:49
date last changed
2017-01-01 07:11:33
@article{6afd16b1-dd05-41ca-af64-204643588c02,
  abstract     = {The aim of this study was to characterize polypeptide components of the capture net spun by trichopteran larvae Hydropsyche slossonae (Banks) (Trichoptera: Hydropsychidae). Thirty-one polypeptide bands were identified by SDS - polyacrylamide gel electrophoresis (SDS-PAGE) from extracted net material, with molecular weights ranging from 8500 to 179 000. Comparison with published data on Bombyx mori (L.) (Lepidoptera: Bombycidae) silk, treated under similar denaturing conditions, shows that six low molecular weight polypeptides ranging between 8500 and 18 800 in the silk of H. slossonae are absent from that of B. mori; furthermore, two high molecular weight polypeptides (210 000 and 220 000) detected in the silk of B. mori are not present in that of H. slossonae. Differences between both groups are probably related to their mode of living and to the specific use of silk (in air versus under water). Our findings are consistent with the current trend in the literature that silk spun by aquatic and terrestrial insects, as well as those spun by different species, is apparently made of different biopolymers according to the protein constituents. Hence, the polypeptide characterization of silk, combined with sequence data and (or) antibodies cross-reactivity data, could represent a potential tool for taxonomic classification improvement of aquatic insects. These results could eventually be used to characterize hydropsychid capture net anomalies induced by environmental pollution.},
  author       = {Tessier, L and Biosvert, J L and Vought, Lena and Lacoursière, Jean},
  issn         = {1918-3240},
  language     = {eng},
  number       = {1},
  pages        = {59--68},
  publisher    = {Entomological Society of Canada},
  series       = {Canadian Entomologist},
  title        = {Characterization of Hydropsyche slossonae (Trichoptera : Hydropsychidae) capture net polypeptides},
  volume       = {132},
  year         = {2000},
}