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Watching DNA condensation induced by poly(amido amine) dendrimers with time-resolved cryo-TEM.

Carnerup, Anna LU ; Ainalem, Marie-Louise LU ; Alfredsson, Viveka LU and Nylander, Tommy LU (2009) In Langmuir 25(21). p.12466-12470
Abstract
The condensation of DNA by poly(amido amine) dendrimers of generation 1, 2, and 4 has been followed by time-resolved cryogenic transmission electron microscopy (cryo-TEM). The recorded images show that significant morphological rearrangement occurs for DNA condensed with the lower generation dendrimers leading to the formation of toroidal aggregates. Higher charge density dendrimers, on the other hand, give rise to globular aggregates, where no transient morphologies are observed. We suggest that the dendrimers in this case are kinetically trapped as soon as they bind to the DNA strand.
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Langmuir
volume
25
issue
21
pages
12466 - 12470
publisher
The American Chemical Society
external identifiers
  • wos:000271106600014
  • pmid:19856988
  • scopus:70449360521
ISSN
0743-7463
DOI
10.1021/la903068v
language
English
LU publication?
yes
id
bb5ee93d-0c1c-4f88-bea3-63cd0fb37682 (old id 1499975)
date added to LUP
2009-11-06 12:12:01
date last changed
2017-02-05 03:27:08
@article{bb5ee93d-0c1c-4f88-bea3-63cd0fb37682,
  abstract     = {The condensation of DNA by poly(amido amine) dendrimers of generation 1, 2, and 4 has been followed by time-resolved cryogenic transmission electron microscopy (cryo-TEM). The recorded images show that significant morphological rearrangement occurs for DNA condensed with the lower generation dendrimers leading to the formation of toroidal aggregates. Higher charge density dendrimers, on the other hand, give rise to globular aggregates, where no transient morphologies are observed. We suggest that the dendrimers in this case are kinetically trapped as soon as they bind to the DNA strand.},
  author       = {Carnerup, Anna and Ainalem, Marie-Louise and Alfredsson, Viveka and Nylander, Tommy},
  issn         = {0743-7463},
  language     = {eng},
  number       = {21},
  pages        = {12466--12470},
  publisher    = {The American Chemical Society},
  series       = {Langmuir},
  title        = {Watching DNA condensation induced by poly(amido amine) dendrimers with time-resolved cryo-TEM.},
  url          = {http://dx.doi.org/10.1021/la903068v},
  volume       = {25},
  year         = {2009},
}