Watching DNA condensation induced by poly(amido amine) dendrimers with time-resolved cryo-TEM.
(2009) In Langmuir 25(21). p.12466-12470- Abstract
- The condensation of DNA by poly(amido amine) dendrimers of generation 1, 2, and 4 has been followed by time-resolved cryogenic transmission electron microscopy (cryo-TEM). The recorded images show that significant morphological rearrangement occurs for DNA condensed with the lower generation dendrimers leading to the formation of toroidal aggregates. Higher charge density dendrimers, on the other hand, give rise to globular aggregates, where no transient morphologies are observed. We suggest that the dendrimers in this case are kinetically trapped as soon as they bind to the DNA strand.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1499975
- author
- Carnerup, Anna LU ; Ainalem, Marie-Louise LU ; Alfredsson, Viveka LU and Nylander, Tommy LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Langmuir
- volume
- 25
- issue
- 21
- pages
- 12466 - 12470
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000271106600014
- pmid:19856988
- scopus:70449360521
- ISSN
- 0743-7463
- DOI
- 10.1021/la903068v
- language
- English
- LU publication?
- yes
- id
- bb5ee93d-0c1c-4f88-bea3-63cd0fb37682 (old id 1499975)
- date added to LUP
- 2016-04-01 11:37:16
- date last changed
- 2022-04-28 17:33:18
@article{bb5ee93d-0c1c-4f88-bea3-63cd0fb37682, abstract = {{The condensation of DNA by poly(amido amine) dendrimers of generation 1, 2, and 4 has been followed by time-resolved cryogenic transmission electron microscopy (cryo-TEM). The recorded images show that significant morphological rearrangement occurs for DNA condensed with the lower generation dendrimers leading to the formation of toroidal aggregates. Higher charge density dendrimers, on the other hand, give rise to globular aggregates, where no transient morphologies are observed. We suggest that the dendrimers in this case are kinetically trapped as soon as they bind to the DNA strand.}}, author = {{Carnerup, Anna and Ainalem, Marie-Louise and Alfredsson, Viveka and Nylander, Tommy}}, issn = {{0743-7463}}, language = {{eng}}, number = {{21}}, pages = {{12466--12470}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Langmuir}}, title = {{Watching DNA condensation induced by poly(amido amine) dendrimers with time-resolved cryo-TEM.}}, url = {{http://dx.doi.org/10.1021/la903068v}}, doi = {{10.1021/la903068v}}, volume = {{25}}, year = {{2009}}, }