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Identification of molecular targets associated with transformed diffuse large B cell lymphoma using highly purified tumor cells.

Andreasson, Ulrika LU ; Dictor, Michael LU ; Jerkeman, Mats LU ; Berglund, Mattias; Sundström, Christer; Linderoth, Johan LU ; Rosenquist, Richard; Borrebaeck, Carl LU and Ek, Sara LU (2009) In American Journal of Hematology 84. p.803-808
Abstract
Follicular lymphoma (FL) frequently transforms into the more aggressive diffuse large B cell lymphoma (DLBCL-tr), but no protein biomarkers have been identified for predictive or early diagnosis. Gene expression analyses have identified genes changing on transformation but have failed to be reproducible in different studies, reflecting the heterogeneity within the tumor tissue and between tumor samples. Gene expression analyses on Affymetrix Human Genome U133 Plus 2.0 arrays were performed, using flow cytometry sorted tumor cells derived from FL and transformed DLBCL. To identify molecular targets associated with the transformation, subsequent immunohistochemistry (IHC) analyses of the corresponding proteins were performed. Using highly... (More)
Follicular lymphoma (FL) frequently transforms into the more aggressive diffuse large B cell lymphoma (DLBCL-tr), but no protein biomarkers have been identified for predictive or early diagnosis. Gene expression analyses have identified genes changing on transformation but have failed to be reproducible in different studies, reflecting the heterogeneity within the tumor tissue and between tumor samples. Gene expression analyses on Affymetrix Human Genome U133 Plus 2.0 arrays were performed, using flow cytometry sorted tumor cells derived from FL and transformed DLBCL. To identify molecular targets associated with the transformation, subsequent immunohistochemistry (IHC) analyses of the corresponding proteins were performed. Using highly purified cells, this study identified 163 genes, which were significantly deregulated during the transformation in a majority of cases. Among the upregulated transcripts, 13 genes were selected for validation using IHC, based on the availability of commercial antibodies, and galectin-3 and NEK2 proteins specifically identify DLBCL-tr, when compared with FL. We demonstrate that by purifying tumor cells through cell sorting, thereby reducing the heterogeneity due to infiltrating cells, it was possible to identify distinct differences between tumor entities rather than variations due to cellular composition. Galectin-3 and NEK2 both identified a subgroup of DLBCL-tr, and the function of these protein markers also suggests a biological role in the transformation process. Am. J. Hematol. 2010. (c) 2009 Wiley-Liss, Inc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
American Journal of Hematology
volume
84
pages
803 - 808
publisher
John Wiley & Sons
external identifiers
  • wos:000272481500008
  • pmid:19844990
  • scopus:73349093689
ISSN
0361-8609
DOI
10.1002/ajh.21549
project
CREATE Health
language
English
LU publication?
yes
id
5e1133d6-a984-4764-a98a-79d7c9aa87b4 (old id 1500136)
date added to LUP
2009-11-04 12:49:14
date last changed
2017-08-13 03:35:27
@article{5e1133d6-a984-4764-a98a-79d7c9aa87b4,
  abstract     = {Follicular lymphoma (FL) frequently transforms into the more aggressive diffuse large B cell lymphoma (DLBCL-tr), but no protein biomarkers have been identified for predictive or early diagnosis. Gene expression analyses have identified genes changing on transformation but have failed to be reproducible in different studies, reflecting the heterogeneity within the tumor tissue and between tumor samples. Gene expression analyses on Affymetrix Human Genome U133 Plus 2.0 arrays were performed, using flow cytometry sorted tumor cells derived from FL and transformed DLBCL. To identify molecular targets associated with the transformation, subsequent immunohistochemistry (IHC) analyses of the corresponding proteins were performed. Using highly purified cells, this study identified 163 genes, which were significantly deregulated during the transformation in a majority of cases. Among the upregulated transcripts, 13 genes were selected for validation using IHC, based on the availability of commercial antibodies, and galectin-3 and NEK2 proteins specifically identify DLBCL-tr, when compared with FL. We demonstrate that by purifying tumor cells through cell sorting, thereby reducing the heterogeneity due to infiltrating cells, it was possible to identify distinct differences between tumor entities rather than variations due to cellular composition. Galectin-3 and NEK2 both identified a subgroup of DLBCL-tr, and the function of these protein markers also suggests a biological role in the transformation process. Am. J. Hematol. 2010. (c) 2009 Wiley-Liss, Inc.},
  author       = {Andreasson, Ulrika and Dictor, Michael and Jerkeman, Mats and Berglund, Mattias and Sundström, Christer and Linderoth, Johan and Rosenquist, Richard and Borrebaeck, Carl and Ek, Sara},
  issn         = {0361-8609},
  language     = {eng},
  pages        = {803--808},
  publisher    = {John Wiley & Sons},
  series       = {American Journal of Hematology},
  title        = {Identification of molecular targets associated with transformed diffuse large B cell lymphoma using highly purified tumor cells.},
  url          = {http://dx.doi.org/10.1002/ajh.21549},
  volume       = {84},
  year         = {2009},
}