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Glucose Generates Coincident Insulin and Somatostatin Pulses and Antisynchronous Glucagon Pulses from Human Pancreatic Islets.

Hellman, Bo; Salehi, S Albert LU ; Gylfe, Erik; Dansk, Heléne and Grapengiesser, Eva (2009) In Endocrinology 150. p.5334-5340
Abstract
The kinetics of insulin, glucagon and somatostatin release was studied in human pancreatic islets. Batches of 10-15 islets were perifused and the hormones measured with RIA in 30-sec fractions. Increase of glucose from 3 to 20 mM resulted in a brief pulse of glucagon coinciding with suppression of basal insulin and somatostatin release. There was a subsequent drop of glucagon release concomitant with the appearance of a pronounced pulse of insulin and a slightly delayed pulse of somatostatin. Continued exposure to 20 mM glucose generated pulsatile release of the three hormones with 7- to 8-min periods accounting for 60-70% of the secreted amounts. Glucose caused pronounced stimulation of average insulin and somatostatin release. However,... (More)
The kinetics of insulin, glucagon and somatostatin release was studied in human pancreatic islets. Batches of 10-15 islets were perifused and the hormones measured with RIA in 30-sec fractions. Increase of glucose from 3 to 20 mM resulted in a brief pulse of glucagon coinciding with suppression of basal insulin and somatostatin release. There was a subsequent drop of glucagon release concomitant with the appearance of a pronounced pulse of insulin and a slightly delayed pulse of somatostatin. Continued exposure to 20 mM glucose generated pulsatile release of the three hormones with 7- to 8-min periods accounting for 60-70% of the secreted amounts. Glucose caused pronounced stimulation of average insulin and somatostatin release. However, the nadirs between the glucagon pulses were lower than the secretion at 3 mM glucose, resulting in 18% suppression of average release. The repetitive glucagon pulses were antisynchronous to coincident pulses of insulin and somatostatin. The resulting greater than 20-fold variations of the insulin to glucagon ratio might be essential for minute-to-minute regulation of the hepatic glucose production. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Endocrinology
volume
150
pages
5334 - 5340
publisher
Endocrine Society
external identifiers
  • wos:000272042300018
  • pmid:19819962
  • scopus:71949101801
ISSN
0013-7227
DOI
10.1210/en.2009-0600
language
English
LU publication?
yes
id
2cc7a2f9-faa9-4e53-8726-799db25c8d38 (old id 1500426)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19819962?dopt=Abstract
date added to LUP
2009-11-03 14:45:06
date last changed
2017-09-24 04:37:58
@article{2cc7a2f9-faa9-4e53-8726-799db25c8d38,
  abstract     = {The kinetics of insulin, glucagon and somatostatin release was studied in human pancreatic islets. Batches of 10-15 islets were perifused and the hormones measured with RIA in 30-sec fractions. Increase of glucose from 3 to 20 mM resulted in a brief pulse of glucagon coinciding with suppression of basal insulin and somatostatin release. There was a subsequent drop of glucagon release concomitant with the appearance of a pronounced pulse of insulin and a slightly delayed pulse of somatostatin. Continued exposure to 20 mM glucose generated pulsatile release of the three hormones with 7- to 8-min periods accounting for 60-70% of the secreted amounts. Glucose caused pronounced stimulation of average insulin and somatostatin release. However, the nadirs between the glucagon pulses were lower than the secretion at 3 mM glucose, resulting in 18% suppression of average release. The repetitive glucagon pulses were antisynchronous to coincident pulses of insulin and somatostatin. The resulting greater than 20-fold variations of the insulin to glucagon ratio might be essential for minute-to-minute regulation of the hepatic glucose production.},
  author       = {Hellman, Bo and Salehi, S Albert and Gylfe, Erik and Dansk, Heléne and Grapengiesser, Eva},
  issn         = {0013-7227},
  language     = {eng},
  pages        = {5334--5340},
  publisher    = {Endocrine Society},
  series       = {Endocrinology},
  title        = {Glucose Generates Coincident Insulin and Somatostatin Pulses and Antisynchronous Glucagon Pulses from Human Pancreatic Islets.},
  url          = {http://dx.doi.org/10.1210/en.2009-0600},
  volume       = {150},
  year         = {2009},
}