Glucose Generates Coincident Insulin and Somatostatin Pulses and Antisynchronous Glucagon Pulses from Human Pancreatic Islets.
Hellman, Bo ; Salehi, S Albert LU
; Gylfe, Erik
; Dansk, Heléne
and Grapengiesser, Eva
(2009)
In Endocrinology
150.
p.5334-5340
- Abstract
- The kinetics of insulin, glucagon and somatostatin release was studied in human pancreatic islets. Batches of 10-15 islets were perifused and the hormones measured with RIA in 30-sec fractions. Increase of glucose from 3 to 20 mM resulted in a brief pulse of glucagon coinciding with suppression of basal insulin and somatostatin release. There was a subsequent drop of glucagon release concomitant with the appearance of a pronounced pulse of insulin and a slightly delayed pulse of somatostatin. Continued exposure to 20 mM glucose generated pulsatile release of the three hormones with 7- to 8-min periods accounting for 60-70% of the secreted amounts. Glucose caused pronounced stimulation of average insulin and somatostatin release. However,... (More)
- The kinetics of insulin, glucagon and somatostatin release was studied in human pancreatic islets. Batches of 10-15 islets were perifused and the hormones measured with RIA in 30-sec fractions. Increase of glucose from 3 to 20 mM resulted in a brief pulse of glucagon coinciding with suppression of basal insulin and somatostatin release. There was a subsequent drop of glucagon release concomitant with the appearance of a pronounced pulse of insulin and a slightly delayed pulse of somatostatin. Continued exposure to 20 mM glucose generated pulsatile release of the three hormones with 7- to 8-min periods accounting for 60-70% of the secreted amounts. Glucose caused pronounced stimulation of average insulin and somatostatin release. However, the nadirs between the glucagon pulses were lower than the secretion at 3 mM glucose, resulting in 18% suppression of average release. The repetitive glucagon pulses were antisynchronous to coincident pulses of insulin and somatostatin. The resulting greater than 20-fold variations of the insulin to glucagon ratio might be essential for minute-to-minute regulation of the hepatic glucose production. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1500426
- author
- Hellman, Bo
; Salehi, S Albert
LU
; Gylfe, Erik
; Dansk, Heléne
and Grapengiesser, Eva
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Endocrinology
- volume
- 150
- pages
- 5334 - 5340
- publisher
- Oxford University Press
- external identifiers
-
- wos:000272042300018
- pmid:19819962
- scopus:71949101801
- pmid:19819962
- ISSN
- 0013-7227
- DOI
- 10.1210/en.2009-0600
- language
- English
- LU publication?
- yes
- id
- 2cc7a2f9-faa9-4e53-8726-799db25c8d38 (old id 1500426)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/19819962?dopt=Abstract
- date added to LUP
- 2016-04-04 08:09:26
- date last changed
- 2022-02-28 04:34:20
@article{2cc7a2f9-faa9-4e53-8726-799db25c8d38,
abstract = {{The kinetics of insulin, glucagon and somatostatin release was studied in human pancreatic islets. Batches of 10-15 islets were perifused and the hormones measured with RIA in 30-sec fractions. Increase of glucose from 3 to 20 mM resulted in a brief pulse of glucagon coinciding with suppression of basal insulin and somatostatin release. There was a subsequent drop of glucagon release concomitant with the appearance of a pronounced pulse of insulin and a slightly delayed pulse of somatostatin. Continued exposure to 20 mM glucose generated pulsatile release of the three hormones with 7- to 8-min periods accounting for 60-70% of the secreted amounts. Glucose caused pronounced stimulation of average insulin and somatostatin release. However, the nadirs between the glucagon pulses were lower than the secretion at 3 mM glucose, resulting in 18% suppression of average release. The repetitive glucagon pulses were antisynchronous to coincident pulses of insulin and somatostatin. The resulting greater than 20-fold variations of the insulin to glucagon ratio might be essential for minute-to-minute regulation of the hepatic glucose production.}},
author = {{Hellman, Bo and Salehi, S Albert and Gylfe, Erik and Dansk, Heléne and Grapengiesser, Eva}},
issn = {{0013-7227}},
language = {{eng}},
pages = {{5334--5340}},
publisher = {{Oxford University Press}},
series = {{Endocrinology}},
title = {{Glucose Generates Coincident Insulin and Somatostatin Pulses and Antisynchronous Glucagon Pulses from Human Pancreatic Islets.}},
url = {{http://dx.doi.org/10.1210/en.2009-0600}},
doi = {{10.1210/en.2009-0600}},
volume = {{150}},
year = {{2009}},
}