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A novel role for constitutively expressed epithelial-derived chemokines as antibacterial peptides in the intestinal mucosa.

Kotarsky, Knut LU ; Sitnik, Katarzyna LU ; Stenstad, Hanna LU ; Kotarsky, Heike LU ; Schmidtchen, Artur LU ; Koslowski, M; Wehkamp, J and Agace, William LU (2010) In Mucosal Immunology 3. p.40-48
Abstract
Intestinal-derived chemokines have a central role in orchestrating immune cell influx into the normal and inflamed intestine. Here, we identify the chemokine CCL6 as one of the most abundant chemokines constitutively expressed by both murine small intestinal and colonic epithelial cells. CCL6 protein localized to crypt epithelial cells, was detected in the gut lumen and reached high concentrations at the mucosal surface. Its expression was further enhanced in the small intestine following in vivo administration of LPS or after stimulation of the small intestinal epithelial cell line, mIC(c12), with IFNgamma, IL-4 or TNFalpha. Recombinant- and intestinal-derived CCL6 bound to a subset of the intestinal microflora and displayed antibacterial... (More)
Intestinal-derived chemokines have a central role in orchestrating immune cell influx into the normal and inflamed intestine. Here, we identify the chemokine CCL6 as one of the most abundant chemokines constitutively expressed by both murine small intestinal and colonic epithelial cells. CCL6 protein localized to crypt epithelial cells, was detected in the gut lumen and reached high concentrations at the mucosal surface. Its expression was further enhanced in the small intestine following in vivo administration of LPS or after stimulation of the small intestinal epithelial cell line, mIC(c12), with IFNgamma, IL-4 or TNFalpha. Recombinant- and intestinal-derived CCL6 bound to a subset of the intestinal microflora and displayed antibacterial activity. Finally, the human homologs to CCL6, CCL14 and CCL15 were also constitutively expressed at high levels in human intestinal epithelium, were further enhanced in inflammatory bowel disease and displayed similar antibacterial activity. These findings identify a novel role for constitutively expressed, epithelial-derived chemokines as antimicrobial peptides in the intestinal mucosa.Mucosal Immunology advance online publication 7 October 2009. doi:10.1038/mi.2009.115. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Mucosal Immunology
volume
3
pages
40 - 48
publisher
Nature Publishing Group
external identifiers
  • wos:000272798800007
  • pmid:19812544
  • scopus:77249094080
ISSN
1933-0219
DOI
10.1038/mi.2009.115
language
English
LU publication?
yes
id
407d25a5-8b4c-4276-b0ca-a82a57450d3b (old id 1500491)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19812544?dopt=Abstract
date added to LUP
2009-11-04 09:30:03
date last changed
2018-05-29 10:14:32
@article{407d25a5-8b4c-4276-b0ca-a82a57450d3b,
  abstract     = {Intestinal-derived chemokines have a central role in orchestrating immune cell influx into the normal and inflamed intestine. Here, we identify the chemokine CCL6 as one of the most abundant chemokines constitutively expressed by both murine small intestinal and colonic epithelial cells. CCL6 protein localized to crypt epithelial cells, was detected in the gut lumen and reached high concentrations at the mucosal surface. Its expression was further enhanced in the small intestine following in vivo administration of LPS or after stimulation of the small intestinal epithelial cell line, mIC(c12), with IFNgamma, IL-4 or TNFalpha. Recombinant- and intestinal-derived CCL6 bound to a subset of the intestinal microflora and displayed antibacterial activity. Finally, the human homologs to CCL6, CCL14 and CCL15 were also constitutively expressed at high levels in human intestinal epithelium, were further enhanced in inflammatory bowel disease and displayed similar antibacterial activity. These findings identify a novel role for constitutively expressed, epithelial-derived chemokines as antimicrobial peptides in the intestinal mucosa.Mucosal Immunology advance online publication 7 October 2009. doi:10.1038/mi.2009.115.},
  author       = {Kotarsky, Knut and Sitnik, Katarzyna and Stenstad, Hanna and Kotarsky, Heike and Schmidtchen, Artur and Koslowski, M and Wehkamp, J and Agace, William},
  issn         = {1933-0219},
  language     = {eng},
  pages        = {40--48},
  publisher    = {Nature Publishing Group},
  series       = {Mucosal Immunology},
  title        = {A novel role for constitutively expressed epithelial-derived chemokines as antibacterial peptides in the intestinal mucosa.},
  url          = {http://dx.doi.org/10.1038/mi.2009.115},
  volume       = {3},
  year         = {2010},
}