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Different Requirements for Early and Late Phases of Azurophilic Granule-Phagosome Fusion.

Nordenfelt, Pontus LU ; Winberg, Martin E; Lönnbro, Per LU ; Rasmusson, Birgitta and Tapper, Hans LU (2009) In Traffic: the International Journal of Intracellular Transport 10. p.1881-1893
Abstract
Abstract Phagocytosis and killing of microorganisms are complex processes that involve tightly regulated membrane traffic events. Because many signaling molecules associate with membrane rafts and because these structures can be found on azurophilic granules, we decided to investigate raft recruitment and the signaling requirements for azurophilic granule secretion during phagosome maturation. At the site of phagocytosis of immunoglobulin G-opsonized prey in human neutrophils, we found that early secretion of azurophilic granules was both raft- and calcium-dependent. Subsequently, rafts at the phagocytic site were internalized with the prey. At the fully formed phagosome, the fusion of azurophilic granules was no longer dependent on rafts... (More)
Abstract Phagocytosis and killing of microorganisms are complex processes that involve tightly regulated membrane traffic events. Because many signaling molecules associate with membrane rafts and because these structures can be found on azurophilic granules, we decided to investigate raft recruitment and the signaling requirements for azurophilic granule secretion during phagosome maturation. At the site of phagocytosis of immunoglobulin G-opsonized prey in human neutrophils, we found that early secretion of azurophilic granules was both raft- and calcium-dependent. Subsequently, rafts at the phagocytic site were internalized with the prey. At the fully formed phagosome, the fusion of azurophilic granules was no longer dependent on rafts or calcium. These findings were found to be true also when using Streptococcus pyogenes bacteria as prey, and depletion of calcium affected the kinetics of bacterial intracellular survival. These findings suggest that the mechanisms for delivery of azurophilic content to nascent and sealed phagosomes, respectively, differ in their dependence on calcium and membrane rafts. (Less)
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author
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type
Contribution to journal
publication status
published
subject
in
Traffic: the International Journal of Intracellular Transport
volume
10
pages
1881 - 1893
publisher
Wiley-Blackwell
external identifiers
  • wos:000271714300014
  • pmid:19804565
  • scopus:70649099317
ISSN
1398-9219
DOI
10.1111/j.1600-0854.2009.00986.x
language
English
LU publication?
yes
id
27dd3dec-8a5a-4a30-be40-8cac7344c8cf (old id 1500664)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/19804565?dopt=Abstract
date added to LUP
2009-11-04 10:39:30
date last changed
2017-02-16 09:13:44
@article{27dd3dec-8a5a-4a30-be40-8cac7344c8cf,
  abstract     = {Abstract Phagocytosis and killing of microorganisms are complex processes that involve tightly regulated membrane traffic events. Because many signaling molecules associate with membrane rafts and because these structures can be found on azurophilic granules, we decided to investigate raft recruitment and the signaling requirements for azurophilic granule secretion during phagosome maturation. At the site of phagocytosis of immunoglobulin G-opsonized prey in human neutrophils, we found that early secretion of azurophilic granules was both raft- and calcium-dependent. Subsequently, rafts at the phagocytic site were internalized with the prey. At the fully formed phagosome, the fusion of azurophilic granules was no longer dependent on rafts or calcium. These findings were found to be true also when using Streptococcus pyogenes bacteria as prey, and depletion of calcium affected the kinetics of bacterial intracellular survival. These findings suggest that the mechanisms for delivery of azurophilic content to nascent and sealed phagosomes, respectively, differ in their dependence on calcium and membrane rafts.},
  author       = {Nordenfelt, Pontus and Winberg, Martin E and Lönnbro, Per and Rasmusson, Birgitta and Tapper, Hans},
  issn         = {1398-9219},
  language     = {eng},
  pages        = {1881--1893},
  publisher    = {Wiley-Blackwell},
  series       = {Traffic: the International Journal of Intracellular Transport},
  title        = {Different Requirements for Early and Late Phases of Azurophilic Granule-Phagosome Fusion.},
  url          = {http://dx.doi.org/10.1111/j.1600-0854.2009.00986.x},
  volume       = {10},
  year         = {2009},
}