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Determination of the self-diffusion coefficient of intracellular water using PGSE NMR with variable gradient pulse length.

Åslund, Ingrid LU and Topgaard, Daniel LU (2009) In Journal of Magnetic Resonance 201(2). p.250-254
Abstract
A new pulsed-gradient spin-echo NMR protocol for assessing the local self-diffusion coefficient D(0) of water confined within living cells is proposed. Equations for the apparent mean-square displacement <Z(2)> as a function of the effective diffusion time t(d) and the duration of the displacement-encoding gradient pulses delta are derived. The standard method of estimating D(0) - reducing t(d) until the influence of collisions between the water molecules and the plasma membrane can be neglected - often fails because of the small size of typical cells. As demonstrated here, the decrease of the apparent <Z(2)> with increasing delta at constant t(d) can be utilized to measure D(0).
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Magnetic Resonance
volume
201
issue
2
pages
250 - 254
publisher
Elsevier
external identifiers
  • wos:000272006600017
  • pmid:19800273
  • scopus:70350617673
ISSN
1096-0856
DOI
10.1016/j.jmr.2009.09.006
language
English
LU publication?
yes
id
ef7e32ba-1b6b-4f38-84cc-d17419856a70 (old id 1500734)
date added to LUP
2009-12-01 10:15:33
date last changed
2017-11-05 03:55:08
@article{ef7e32ba-1b6b-4f38-84cc-d17419856a70,
  abstract     = {A new pulsed-gradient spin-echo NMR protocol for assessing the local self-diffusion coefficient D(0) of water confined within living cells is proposed. Equations for the apparent mean-square displacement &lt;Z(2)&gt; as a function of the effective diffusion time t(d) and the duration of the displacement-encoding gradient pulses delta are derived. The standard method of estimating D(0) - reducing t(d) until the influence of collisions between the water molecules and the plasma membrane can be neglected - often fails because of the small size of typical cells. As demonstrated here, the decrease of the apparent &lt;Z(2)&gt; with increasing delta at constant t(d) can be utilized to measure D(0).},
  author       = {Åslund, Ingrid and Topgaard, Daniel},
  issn         = {1096-0856},
  language     = {eng},
  number       = {2},
  pages        = {250--254},
  publisher    = {Elsevier},
  series       = {Journal of Magnetic Resonance},
  title        = {Determination of the self-diffusion coefficient of intracellular water using PGSE NMR with variable gradient pulse length.},
  url          = {http://dx.doi.org/10.1016/j.jmr.2009.09.006},
  volume       = {201},
  year         = {2009},
}