Advanced

Origin and destination of globules and irregular masses in the gonadotropin cells from the pituitary of the African catfish, Clarias gariepinus: a morphological study

Sharp-Baker, H. E.; Peute, J.; Diederen, J. H. B. and Brokken, Leon LU (1995) In Cell and Tissue Research 280(1). p.113-122
Abstract
The possible function of globules and irregular membrane-bound masses in the gonadotropin cells of the pituitary of Clarias gariepinus was studied. Strong secretory stimulation led to the disappearance of the secretory granules from gonadotropin cells but globules and irregular masses remained present. Acid phosphatase was detected enzyme-cytochemically in both globules and irregular masses. Radiolabelling with tritiated amino acids followed by autoradiography demonstrated that globules received radioactive material after secretory granules. The latter received radioactive material within 75 min of administration of radioactive amino acids but globules and irregular masses did not. Although some globules became radioactively labelled... (More)
The possible function of globules and irregular membrane-bound masses in the gonadotropin cells of the pituitary of Clarias gariepinus was studied. Strong secretory stimulation led to the disappearance of the secretory granules from gonadotropin cells but globules and irregular masses remained present. Acid phosphatase was detected enzyme-cytochemically in both globules and irregular masses. Radiolabelling with tritiated amino acids followed by autoradiography demonstrated that globules received radioactive material after secretory granules. The latter received radioactive material within 75 min of administration of radioactive amino acids but globules and irregular masses did not. Although some globules became radioactively labelled within 24 h of the administration of radioactive amino acids, irregular masses remained unlabelled during this period. Secretory granules reacted positively with antisera against a and ß gonadotropin subunits, whereas globules and irregular masses only reacted with the antiserum against the ß subunit. A moderate anti-7B2 immunoreactivity was demonstrated in secretory granules and globules, whereas irregular masses labelled strongly. The combined cytological results indicate that globules and irregular masses are degradative, possibly crinophagic structures which develop by fusional events from secretory granules to globules and then to irregular masses. (Less)
Please use this url to cite or link to this publication:
author
publishing date
type
Contribution to journal
publication status
published
subject
in
Cell and Tissue Research
volume
280
issue
1
pages
113 - 122
publisher
Springer
external identifiers
  • scopus:0028930083
ISSN
1432-0878
DOI
10.1007/BF00304516
language
English
LU publication?
no
id
982d2a70-2d19-4aaf-8b2c-ef064a7a31db (old id 1501034)
alternative location
http://link.springer.com/article/10.1007%2FBF00304516
date added to LUP
2013-08-06 15:20:05
date last changed
2017-01-01 05:07:05
@article{982d2a70-2d19-4aaf-8b2c-ef064a7a31db,
  abstract     = {The possible function of globules and irregular membrane-bound masses in the gonadotropin cells of the pituitary of Clarias gariepinus was studied. Strong secretory stimulation led to the disappearance of the secretory granules from gonadotropin cells but globules and irregular masses remained present. Acid phosphatase was detected enzyme-cytochemically in both globules and irregular masses. Radiolabelling with tritiated amino acids followed by autoradiography demonstrated that globules received radioactive material after secretory granules. The latter received radioactive material within 75 min of administration of radioactive amino acids but globules and irregular masses did not. Although some globules became radioactively labelled within 24 h of the administration of radioactive amino acids, irregular masses remained unlabelled during this period. Secretory granules reacted positively with antisera against a and ß gonadotropin subunits, whereas globules and irregular masses only reacted with the antiserum against the ß subunit. A moderate anti-7B2 immunoreactivity was demonstrated in secretory granules and globules, whereas irregular masses labelled strongly. The combined cytological results indicate that globules and irregular masses are degradative, possibly crinophagic structures which develop by fusional events from secretory granules to globules and then to irregular masses.},
  author       = {Sharp-Baker, H. E. and Peute, J. and Diederen, J. H. B. and Brokken, Leon},
  issn         = {1432-0878},
  language     = {eng},
  number       = {1},
  pages        = {113--122},
  publisher    = {Springer},
  series       = {Cell and Tissue Research},
  title        = {Origin and destination of globules and irregular masses in the gonadotropin cells from the pituitary of the African catfish, Clarias gariepinus: a morphological study},
  url          = {http://dx.doi.org/10.1007/BF00304516},
  volume       = {280},
  year         = {1995},
}