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Mapping the mechanics and macromolecular organization of hyaluronan-rich cell coats

Boehm, Heike; Mundinger, Tabea A.; Boehm, Christian H. J.; Hagel, Valentin; Rauch, Uwe LU ; Spatz, Joachim P. and Curtis, Jennifer E. (2009) In Soft Matter 5(21). p.4331-4337
Abstract
The hyaluronan (HA)-rich pericellular coat (PCC) enveloping most mammalian cells plays a vital role in biological processes such as cell adhesion, proliferation, motility and embryogenesis. In particular its presence on chondrocytes, which live in the load-bearing cartilage, has a wide range of implications in diseases such as osteoarthritis, highlighting its mechanical role in living organisms. Despite its significance, the macromolecular organization of the cell coat remains speculative. In order to obtain a more detailed spatial picture of highly hydrated PCCs, we present two independent but complementary non-invasive techniques for the position-resolved analysis of the cell coat's mechanical and structural properties.... (More)
The hyaluronan (HA)-rich pericellular coat (PCC) enveloping most mammalian cells plays a vital role in biological processes such as cell adhesion, proliferation, motility and embryogenesis. In particular its presence on chondrocytes, which live in the load-bearing cartilage, has a wide range of implications in diseases such as osteoarthritis, highlighting its mechanical role in living organisms. Despite its significance, the macromolecular organization of the cell coat remains speculative. In order to obtain a more detailed spatial picture of highly hydrated PCCs, we present two independent but complementary non-invasive techniques for the position-resolved analysis of the cell coat's mechanical and structural properties. Position-dependent microrheology provides a micromechanical map of the PCC that reveals a gradient of increasing elastic stiffness towards the plasma membrane on model rat chondrocyte cells (RCJ-P). This gradient can be correlated with the relative distribution of HA, which is inferred using an eGFP-labelled neurocan-binding domain, a small fluorescent molecule that binds to HA. The spatial variation of the HA concentration profile is consistent with the position-dependent elasticity. Combining these approaches sheds light on the molecular architecture of the PCC. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Soft Matter
volume
5
issue
21
pages
4331 - 4337
publisher
Royal Society of Chemistry
external identifiers
  • wos:000270837900035
  • scopus:70350087358
ISSN
1744-6848
DOI
10.1039/b905574f
language
English
LU publication?
yes
id
df0c8ae2-a2b9-4f37-b441-c284c5a68942 (old id 1505956)
date added to LUP
2009-11-24 10:05:01
date last changed
2017-01-01 05:34:44
@article{df0c8ae2-a2b9-4f37-b441-c284c5a68942,
  abstract     = {The hyaluronan (HA)-rich pericellular coat (PCC) enveloping most mammalian cells plays a vital role in biological processes such as cell adhesion, proliferation, motility and embryogenesis. In particular its presence on chondrocytes, which live in the load-bearing cartilage, has a wide range of implications in diseases such as osteoarthritis, highlighting its mechanical role in living organisms. Despite its significance, the macromolecular organization of the cell coat remains speculative. In order to obtain a more detailed spatial picture of highly hydrated PCCs, we present two independent but complementary non-invasive techniques for the position-resolved analysis of the cell coat's mechanical and structural properties. Position-dependent microrheology provides a micromechanical map of the PCC that reveals a gradient of increasing elastic stiffness towards the plasma membrane on model rat chondrocyte cells (RCJ-P). This gradient can be correlated with the relative distribution of HA, which is inferred using an eGFP-labelled neurocan-binding domain, a small fluorescent molecule that binds to HA. The spatial variation of the HA concentration profile is consistent with the position-dependent elasticity. Combining these approaches sheds light on the molecular architecture of the PCC.},
  author       = {Boehm, Heike and Mundinger, Tabea A. and Boehm, Christian H. J. and Hagel, Valentin and Rauch, Uwe and Spatz, Joachim P. and Curtis, Jennifer E.},
  issn         = {1744-6848},
  language     = {eng},
  number       = {21},
  pages        = {4331--4337},
  publisher    = {Royal Society of Chemistry},
  series       = {Soft Matter},
  title        = {Mapping the mechanics and macromolecular organization of hyaluronan-rich cell coats},
  url          = {http://dx.doi.org/10.1039/b905574f},
  volume       = {5},
  year         = {2009},
}