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A cubic DNA-lipid complex

Bilalov, Azat LU ; Olsson, Ulf LU and Lindman, Björn LU (2009) In Soft Matter 5(20). p.3827-3830
Abstract
DNA-lipid interactions play a significant biological and biotechnological role but remain poorly understood. In investigations of mixed lipid-DNA systems a novel bicontinuous cubic phase, composed of lecithin, water and dodecyltrimethylammonium with DNA as the counterion (DNA-DTA), was identified. The cubic phase is stable only in a narrow composition range and in the temperature range 20-65 degrees C. The samples have a transparent rubber-like morphology, are rigid, optically isotropic and show a shear-induced optical birefringence. The NMR spectrum consists of a broadened singlet. Upon increasing the lecithin content the phase transforms to lamellar phase. The liquid crystal (LC) is determined to be in Ia3d symmetry from investigations... (More)
DNA-lipid interactions play a significant biological and biotechnological role but remain poorly understood. In investigations of mixed lipid-DNA systems a novel bicontinuous cubic phase, composed of lecithin, water and dodecyltrimethylammonium with DNA as the counterion (DNA-DTA), was identified. The cubic phase is stable only in a narrow composition range and in the temperature range 20-65 degrees C. The samples have a transparent rubber-like morphology, are rigid, optically isotropic and show a shear-induced optical birefringence. The NMR spectrum consists of a broadened singlet. Upon increasing the lecithin content the phase transforms to lamellar phase. The liquid crystal (LC) is determined to be in Ia3d symmetry from investigations by small angle X-ray scattering (SAXS). Model calculations indicate that the cubic phase is normal, i.e. being composed of DNA chains and water incorporated in polar group bilayers separating two hydrophobic domains containing hydrocarbon tails of lipids. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Soft Matter
volume
5
issue
20
pages
3827 - 3830
publisher
Royal Society of Chemistry
external identifiers
  • wos:000270542600006
  • scopus:77956940252
ISSN
1744-6848
DOI
10.1039/b908939j
language
English
LU publication?
yes
id
ca53fde9-016a-47d6-992f-4c6d85f99350 (old id 1507207)
date added to LUP
2009-11-20 15:29:52
date last changed
2017-10-01 04:25:07
@article{ca53fde9-016a-47d6-992f-4c6d85f99350,
  abstract     = {DNA-lipid interactions play a significant biological and biotechnological role but remain poorly understood. In investigations of mixed lipid-DNA systems a novel bicontinuous cubic phase, composed of lecithin, water and dodecyltrimethylammonium with DNA as the counterion (DNA-DTA), was identified. The cubic phase is stable only in a narrow composition range and in the temperature range 20-65 degrees C. The samples have a transparent rubber-like morphology, are rigid, optically isotropic and show a shear-induced optical birefringence. The NMR spectrum consists of a broadened singlet. Upon increasing the lecithin content the phase transforms to lamellar phase. The liquid crystal (LC) is determined to be in Ia3d symmetry from investigations by small angle X-ray scattering (SAXS). Model calculations indicate that the cubic phase is normal, i.e. being composed of DNA chains and water incorporated in polar group bilayers separating two hydrophobic domains containing hydrocarbon tails of lipids.},
  author       = {Bilalov, Azat and Olsson, Ulf and Lindman, Björn},
  issn         = {1744-6848},
  language     = {eng},
  number       = {20},
  pages        = {3827--3830},
  publisher    = {Royal Society of Chemistry},
  series       = {Soft Matter},
  title        = {A cubic DNA-lipid complex},
  url          = {http://dx.doi.org/10.1039/b908939j},
  volume       = {5},
  year         = {2009},
}