A cubic DNA-lipid complex
(2009) In Soft Matter 5(20). p.3827-3830- Abstract
- DNA-lipid interactions play a significant biological and biotechnological role but remain poorly understood. In investigations of mixed lipid-DNA systems a novel bicontinuous cubic phase, composed of lecithin, water and dodecyltrimethylammonium with DNA as the counterion (DNA-DTA), was identified. The cubic phase is stable only in a narrow composition range and in the temperature range 20-65 degrees C. The samples have a transparent rubber-like morphology, are rigid, optically isotropic and show a shear-induced optical birefringence. The NMR spectrum consists of a broadened singlet. Upon increasing the lecithin content the phase transforms to lamellar phase. The liquid crystal (LC) is determined to be in Ia3d symmetry from investigations... (More)
- DNA-lipid interactions play a significant biological and biotechnological role but remain poorly understood. In investigations of mixed lipid-DNA systems a novel bicontinuous cubic phase, composed of lecithin, water and dodecyltrimethylammonium with DNA as the counterion (DNA-DTA), was identified. The cubic phase is stable only in a narrow composition range and in the temperature range 20-65 degrees C. The samples have a transparent rubber-like morphology, are rigid, optically isotropic and show a shear-induced optical birefringence. The NMR spectrum consists of a broadened singlet. Upon increasing the lecithin content the phase transforms to lamellar phase. The liquid crystal (LC) is determined to be in Ia3d symmetry from investigations by small angle X-ray scattering (SAXS). Model calculations indicate that the cubic phase is normal, i.e. being composed of DNA chains and water incorporated in polar group bilayers separating two hydrophobic domains containing hydrocarbon tails of lipids. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1507207
- author
- Bilalov, Azat LU ; Olsson, Ulf LU and Lindman, Björn LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Soft Matter
- volume
- 5
- issue
- 20
- pages
- 3827 - 3830
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000270542600006
- scopus:77956940252
- ISSN
- 1744-6848
- DOI
- 10.1039/b908939j
- language
- English
- LU publication?
- yes
- id
- ca53fde9-016a-47d6-992f-4c6d85f99350 (old id 1507207)
- date added to LUP
- 2016-04-01 14:36:05
- date last changed
- 2022-01-28 01:30:26
@article{ca53fde9-016a-47d6-992f-4c6d85f99350,
abstract = {{DNA-lipid interactions play a significant biological and biotechnological role but remain poorly understood. In investigations of mixed lipid-DNA systems a novel bicontinuous cubic phase, composed of lecithin, water and dodecyltrimethylammonium with DNA as the counterion (DNA-DTA), was identified. The cubic phase is stable only in a narrow composition range and in the temperature range 20-65 degrees C. The samples have a transparent rubber-like morphology, are rigid, optically isotropic and show a shear-induced optical birefringence. The NMR spectrum consists of a broadened singlet. Upon increasing the lecithin content the phase transforms to lamellar phase. The liquid crystal (LC) is determined to be in Ia3d symmetry from investigations by small angle X-ray scattering (SAXS). Model calculations indicate that the cubic phase is normal, i.e. being composed of DNA chains and water incorporated in polar group bilayers separating two hydrophobic domains containing hydrocarbon tails of lipids.}},
author = {{Bilalov, Azat and Olsson, Ulf and Lindman, Björn}},
issn = {{1744-6848}},
language = {{eng}},
number = {{20}},
pages = {{3827--3830}},
publisher = {{Royal Society of Chemistry}},
series = {{Soft Matter}},
title = {{A cubic DNA-lipid complex}},
url = {{http://dx.doi.org/10.1039/b908939j}},
doi = {{10.1039/b908939j}},
volume = {{5}},
year = {{2009}},
}