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LPS induces GROalpha chemokine production via NF-kappaB in oral fibroblasts.

Jönsson, Daniel LU ; Amisten, Stefan LU ; Bratthall, G.; Holm, Anders LU and Nilsson, Bengt-Olof LU (2009) In Inflammation Research1995-01-01+01:00 58(11). p.791-796
Abstract
Objective and design Chemotaxis of neutrophils from blood to the inflammation process plays an important role in development of periodontal inflammation. The novel chemokine GRO alpha, also named CXCL1, is a strong chemoattractant for neutrophils. Data on production and regulation of GRO alpha by oral fibroblasts have not previously been presented. Materials and methods GRO alpha mRNA and protein levels were determined in human periodontal ligament cells and mouse gingival fibroblasts by quantitative real-time PCR and ELISA. Results We disclose that both human periodontal ligament cells and mouse gingival fibroblasts produce GRO alpha in response to LPS stimulation. Stimulation with LPS for 24 h increased both mRNA for GRO alpha and GRO... (More)
Objective and design Chemotaxis of neutrophils from blood to the inflammation process plays an important role in development of periodontal inflammation. The novel chemokine GRO alpha, also named CXCL1, is a strong chemoattractant for neutrophils. Data on production and regulation of GRO alpha by oral fibroblasts have not previously been presented. Materials and methods GRO alpha mRNA and protein levels were determined in human periodontal ligament cells and mouse gingival fibroblasts by quantitative real-time PCR and ELISA. Results We disclose that both human periodontal ligament cells and mouse gingival fibroblasts produce GRO alpha in response to LPS stimulation. Stimulation with LPS for 24 h increased both mRNA for GRO alpha and GRO alpha protein. The steroid hormone estrogen had no effect on LPS-induced GRO alpha mRNA expression. Treatment with the glucocorticoid dexamethasone attenuated LPS-induced GRO alpha production, and the NF-kappa B blocker MG 132 fully prevented LPS-induced GRO alpha. Conclusions Oral fibroblasts respond to LPS stimulation by increasing GRO alpha production via the transcription factor NF-kappa B, suggesting that this mechanism may be involved in development of periodontal inflammation. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Estrogen, Chemokines, Dexamethasone, GRO alpha, PDL cells
in
Inflammation Research1995-01-01+01:00
volume
58
issue
11
pages
791 - 796
publisher
Birkhaüser
external identifiers
  • wos:000270652900008
  • scopus:70349917579
  • pmid:19430878
ISSN
1420-908X
DOI
10.1007/s00011-009-0049-z
language
English
LU publication?
yes
id
eec74461-4bde-4cb1-b426-05c4bc5c8e64 (old id 1507366)
date added to LUP
2009-11-20 14:14:33
date last changed
2017-08-13 03:32:14
@article{eec74461-4bde-4cb1-b426-05c4bc5c8e64,
  abstract     = {Objective and design Chemotaxis of neutrophils from blood to the inflammation process plays an important role in development of periodontal inflammation. The novel chemokine GRO alpha, also named CXCL1, is a strong chemoattractant for neutrophils. Data on production and regulation of GRO alpha by oral fibroblasts have not previously been presented. Materials and methods GRO alpha mRNA and protein levels were determined in human periodontal ligament cells and mouse gingival fibroblasts by quantitative real-time PCR and ELISA. Results We disclose that both human periodontal ligament cells and mouse gingival fibroblasts produce GRO alpha in response to LPS stimulation. Stimulation with LPS for 24 h increased both mRNA for GRO alpha and GRO alpha protein. The steroid hormone estrogen had no effect on LPS-induced GRO alpha mRNA expression. Treatment with the glucocorticoid dexamethasone attenuated LPS-induced GRO alpha production, and the NF-kappa B blocker MG 132 fully prevented LPS-induced GRO alpha. Conclusions Oral fibroblasts respond to LPS stimulation by increasing GRO alpha production via the transcription factor NF-kappa B, suggesting that this mechanism may be involved in development of periodontal inflammation.},
  author       = {Jönsson, Daniel and Amisten, Stefan and Bratthall, G. and Holm, Anders and Nilsson, Bengt-Olof},
  issn         = {1420-908X},
  keyword      = {Estrogen,Chemokines,Dexamethasone,GRO alpha,PDL cells},
  language     = {eng},
  number       = {11},
  pages        = {791--796},
  publisher    = {Birkhaüser},
  series       = {Inflammation Research1995-01-01+01:00},
  title        = {LPS induces GROalpha chemokine production via NF-kappaB in oral fibroblasts.},
  url          = {http://dx.doi.org/10.1007/s00011-009-0049-z},
  volume       = {58},
  year         = {2009},
}