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Spraying enzymes in microemulsions of AOT in nonpolar organic solvents for fabrication of enzyme electrodes

Shipovskov, Stepan LU ; Trofimova, D; Saprykin, E; Christenson, Andreas LU ; Ruzgas, Tautgirdas LU ; Levashov, A V and Ferapontova, Elena LU (2005) In Analytical Chemistry 77(21). p.7074-7079
Abstract
A new technique suitable for automated, large-scale fabrication of enzyme electrodes by air-spraying enzymes in organic inks is presented. Model oxidoreductases, tyrosinase (Tyr) and glucose oxidase (GOx), were adapted to octane-based ink by entrapment in a system of reverse micelles (RM) of surfactant AOT in octane to separate and stabilize the catalytically active forms of the enzymes in nonpolar organic media. Nonpolar caoutchouk polymer was also used to create a kind of "dry micelles" at the electrode/solution interface. Enzyme/RM/polymer-containing organic inks were air-brushed onto conductive supports and were subsequently covered by sprayed Nafion membranes. The air-brushed enzyme electrodes exhibited relevant bioelectrocatalytic... (More)
A new technique suitable for automated, large-scale fabrication of enzyme electrodes by air-spraying enzymes in organic inks is presented. Model oxidoreductases, tyrosinase (Tyr) and glucose oxidase (GOx), were adapted to octane-based ink by entrapment in a system of reverse micelles (RM) of surfactant AOT in octane to separate and stabilize the catalytically active forms of the enzymes in nonpolar organic media. Nonpolar caoutchouk polymer was also used to create a kind of "dry micelles" at the electrode/solution interface. Enzyme/RM/polymer-containing organic inks were air-brushed onto conductive supports and were subsequently covered by sprayed Nafion membranes. The air-brushed enzyme electrodes exhibited relevant bioelectrocatalytic activity toward catechol and glucose, with a linear detection range of 0.1-100 mu M catechol and 0.5-7 mM glucose; the sensitivities were 2.41 A M-1 cm(-2) and 2.98 mA M-1 cm(-2) for Tyr and GOx electrodes, respectively. The proposed technique of air-brushing enzymes in organic inks enables automated construction of disposable enzyme electrodes of various designs on a mass-production scale. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
77
issue
21
pages
7074 - 7079
publisher
The American Chemical Society
external identifiers
  • wos:000233125400053
  • scopus:27544478114
ISSN
1520-6882
DOI
10.1021/ac050505d
language
English
LU publication?
yes
id
1a9585d1-de06-4e91-9431-50f1da8cbd50 (old id 150881)
date added to LUP
2007-06-27 15:44:19
date last changed
2017-06-25 03:27:10
@article{1a9585d1-de06-4e91-9431-50f1da8cbd50,
  abstract     = {A new technique suitable for automated, large-scale fabrication of enzyme electrodes by air-spraying enzymes in organic inks is presented. Model oxidoreductases, tyrosinase (Tyr) and glucose oxidase (GOx), were adapted to octane-based ink by entrapment in a system of reverse micelles (RM) of surfactant AOT in octane to separate and stabilize the catalytically active forms of the enzymes in nonpolar organic media. Nonpolar caoutchouk polymer was also used to create a kind of "dry micelles" at the electrode/solution interface. Enzyme/RM/polymer-containing organic inks were air-brushed onto conductive supports and were subsequently covered by sprayed Nafion membranes. The air-brushed enzyme electrodes exhibited relevant bioelectrocatalytic activity toward catechol and glucose, with a linear detection range of 0.1-100 mu M catechol and 0.5-7 mM glucose; the sensitivities were 2.41 A M-1 cm(-2) and 2.98 mA M-1 cm(-2) for Tyr and GOx electrodes, respectively. The proposed technique of air-brushing enzymes in organic inks enables automated construction of disposable enzyme electrodes of various designs on a mass-production scale.},
  author       = {Shipovskov, Stepan and Trofimova, D and Saprykin, E and Christenson, Andreas and Ruzgas, Tautgirdas and Levashov, A V and Ferapontova, Elena},
  issn         = {1520-6882},
  language     = {eng},
  number       = {21},
  pages        = {7074--7079},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Spraying enzymes in microemulsions of AOT in nonpolar organic solvents for fabrication of enzyme electrodes},
  url          = {http://dx.doi.org/10.1021/ac050505d},
  volume       = {77},
  year         = {2005},
}