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Isolation of obligate anaerobic rumen bacteria capable of degrading the neurotoxin beta-ODAP (beta-N-oxalyl-L-alpha,beta-diaminopropionic acid) as evaluated by a liquid chromatography/biosensor analysis system

Marichamy, Sankar LU ; Yigzaw, Yirgalem LU ; Gorton, Lo LU and Mattiasson, Bo LU (2005) In Journal of the Science of Food and Agriculture 85(12). p.2027-2032
Abstract
Six pure strains of obligate anaerobes capable of degrading the toxin beta-N-oxalyl-L-alpha, beta-diaminopropionic acid (beta-ODAP) contained in grass pea (Lathyrus sativus) have been isolated from cow rumen. The new isolates were identified as Megasphaera elsdenii (five different genotypes) and Clostridium bifermentans using 16S rDNA analysis. The beta-ODAP degrading efficiency of the isolates was evaluated by measuring the amount of beta-ODAP in the growth medium, which contained beta-ODAP as the only carbon source, before and after incubation with the microbes. The method of analysis was liquid chromatography employing bioelectrochemical detection. The biosensor is based on coimmobilising two enzymes, glutamate oxidase (GlOx) and... (More)
Six pure strains of obligate anaerobes capable of degrading the toxin beta-N-oxalyl-L-alpha, beta-diaminopropionic acid (beta-ODAP) contained in grass pea (Lathyrus sativus) have been isolated from cow rumen. The new isolates were identified as Megasphaera elsdenii (five different genotypes) and Clostridium bifermentans using 16S rDNA analysis. The beta-ODAP degrading efficiency of the isolates was evaluated by measuring the amount of beta-ODAP in the growth medium, which contained beta-ODAP as the only carbon source, before and after incubation with the microbes. The method of analysis was liquid chromatography employing bioelectrochemical detection. The biosensor is based on coimmobilising two enzymes, glutamate oxidase (GlOx) and horseradish peroxidase (HRP), on the end of a spectrographic graphite electrode. beta-ODAP is oxidised by GlOx to form H2O2, which in turn is bioelectrocatalytically reduced by HRP through a mediated reaction using a polymeric mediator incorporating Os2+/3+ functionalities rapidly shuttling electrons with the electrode-giving rise to the analytical signal. On the basis of this analysis system, the new isolates are capable of utilising beta-ODAP as sole carbon source to a maximum of 90-95% within 5 days with concomitant increase in cell protein. (c) 2005 Society of Chemical Industry. (Less)
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of the Science of Food and Agriculture
volume
85
issue
12
pages
2027 - 2032
publisher
Wiley-Blackwell
external identifiers
  • wos:000231633800010
  • scopus:24344495816
ISSN
1097-0010
DOI
10.1002/jsfa.2211
language
English
LU publication?
yes
id
e42e61d9-50a3-43a4-af35-dcb90ba523f5 (old id 150974)
date added to LUP
2007-06-27 11:02:07
date last changed
2017-08-06 04:30:35
@article{e42e61d9-50a3-43a4-af35-dcb90ba523f5,
  abstract     = {Six pure strains of obligate anaerobes capable of degrading the toxin beta-N-oxalyl-L-alpha, beta-diaminopropionic acid (beta-ODAP) contained in grass pea (Lathyrus sativus) have been isolated from cow rumen. The new isolates were identified as Megasphaera elsdenii (five different genotypes) and Clostridium bifermentans using 16S rDNA analysis. The beta-ODAP degrading efficiency of the isolates was evaluated by measuring the amount of beta-ODAP in the growth medium, which contained beta-ODAP as the only carbon source, before and after incubation with the microbes. The method of analysis was liquid chromatography employing bioelectrochemical detection. The biosensor is based on coimmobilising two enzymes, glutamate oxidase (GlOx) and horseradish peroxidase (HRP), on the end of a spectrographic graphite electrode. beta-ODAP is oxidised by GlOx to form H2O2, which in turn is bioelectrocatalytically reduced by HRP through a mediated reaction using a polymeric mediator incorporating Os2+/3+ functionalities rapidly shuttling electrons with the electrode-giving rise to the analytical signal. On the basis of this analysis system, the new isolates are capable of utilising beta-ODAP as sole carbon source to a maximum of 90-95% within 5 days with concomitant increase in cell protein. (c) 2005 Society of Chemical Industry.},
  author       = {Marichamy, Sankar and Yigzaw, Yirgalem and Gorton, Lo and Mattiasson, Bo},
  issn         = {1097-0010},
  language     = {eng},
  number       = {12},
  pages        = {2027--2032},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of the Science of Food and Agriculture},
  title        = {Isolation of obligate anaerobic rumen bacteria capable of degrading the neurotoxin beta-ODAP (beta-N-oxalyl-L-alpha,beta-diaminopropionic acid) as evaluated by a liquid chromatography/biosensor analysis system},
  url          = {http://dx.doi.org/10.1002/jsfa.2211},
  volume       = {85},
  year         = {2005},
}