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Introduction to biological mass spectrometry: Determining identity and species of origin of two proteins

Reimann, Curt LU ; Mie, Axel LU ; Nilsson, Carina LU and Cohen, Arieh LU (2005) In Journal of Chemical Education 82(8). p.1215-1218
Abstract
We describe a laboratory exercise that is designed to give advanced undergraduate students in analytical chemistry a meaningful introduction to biological mass spectrometry. We ask the students to solve a mystery: given two proteins, what are they, and from which animals do they originate? This exercise involves use of two mass spectrometers available in our institution: a fairly basic matrix-assisted laser desorption ionization time-of-flight (MALDI–TOF) unit and an electrospray ionization (ESI) ion-trap unit. Our exercise can be completed in two days (including analysis) and provides several interesting and educational surprises. The students, in small groups, are introduced to total mass measurement with ESI, proceed to peptide mass... (More)
We describe a laboratory exercise that is designed to give advanced undergraduate students in analytical chemistry a meaningful introduction to biological mass spectrometry. We ask the students to solve a mystery: given two proteins, what are they, and from which animals do they originate? This exercise involves use of two mass spectrometers available in our institution: a fairly basic matrix-assisted laser desorption ionization time-of-flight (MALDI–TOF) unit and an electrospray ionization (ESI) ion-trap unit. Our exercise can be completed in two days (including analysis) and provides several interesting and educational surprises. The students, in small groups, are introduced to total mass measurement with ESI, proceed to peptide mass mapping with MALDI–TOF, and finally explore sequence information with ion-trap tandem (fragmentation) mass spectrometry. Even in the most advanced phase, we guide the students, but by this time they usually have their own ideas as to what avenues to pursue. We find that our students become excited after they solve the "mystery"of these proteins, and they receive enough education in the process to be able to join a research project involving biological mass spectrometry. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Journal of Chemical Education
volume
82
issue
8
pages
1215 - 1218
publisher
The American Chemical Society
external identifiers
  • wos:000230458700025
  • scopus:22944457848
ISSN
0021-9584
language
English
LU publication?
yes
id
b56a1149-6d88-44bf-8c95-daa33d353ff5 (old id 150989)
alternative location
http://portal.isiknowledge.com/portal.cgi/wos?SID=P21pPbNgc73D43HELg@
date added to LUP
2007-06-27 14:03:09
date last changed
2017-01-01 07:28:53
@article{b56a1149-6d88-44bf-8c95-daa33d353ff5,
  abstract     = {We describe a laboratory exercise that is designed to give advanced undergraduate students in analytical chemistry a meaningful introduction to biological mass spectrometry. We ask the students to solve a mystery: given two proteins, what are they, and from which animals do they originate? This exercise involves use of two mass spectrometers available in our institution: a fairly basic matrix-assisted laser desorption ionization time-of-flight (MALDI–TOF) unit and an electrospray ionization (ESI) ion-trap unit. Our exercise can be completed in two days (including analysis) and provides several interesting and educational surprises. The students, in small groups, are introduced to total mass measurement with ESI, proceed to peptide mass mapping with MALDI–TOF, and finally explore sequence information with ion-trap tandem (fragmentation) mass spectrometry. Even in the most advanced phase, we guide the students, but by this time they usually have their own ideas as to what avenues to pursue. We find that our students become excited after they solve the "mystery"of these proteins, and they receive enough education in the process to be able to join a research project involving biological mass spectrometry.},
  author       = {Reimann, Curt and Mie, Axel and Nilsson, Carina and Cohen, Arieh},
  issn         = {0021-9584},
  language     = {eng},
  number       = {8},
  pages        = {1215--1218},
  publisher    = {The American Chemical Society},
  series       = {Journal of Chemical Education},
  title        = {Introduction to biological mass spectrometry: Determining identity and species of origin of two proteins},
  volume       = {82},
  year         = {2005},
}