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An amperometric biosensor based on laccase immobilized in polymer matrices for determining phenolic compounds

Yaropolov, A I; Shleev, Sergey LU ; Morozova, O V; Zaitseva, E A; Marko-Varga, G; Emnéus, Jenny LU and Gorton, Lo LU (2005) In Journal of Analytical Chemistry 60(6). p.553-557
Abstract
An amperometric enzyme electrode based on laccase for determining phenolic compounds is proposed. The following three types of polymer materials were used for enzyme immobilization on the surface of a glassy-carbon electrode: positively charged cetyl ethyl poly (ethyleneimine) (CEPEI) and negatively charged commercial Nafion and Eastman AQ 29D polymers. The advantages and disadvantages of each of the above polymers for enzyme immobilization are discussed. The detection limits of the model phenolic compounds hydroquinone and pyrocatechol in a buffer solution on laccase immobilization in a Nation membrane were 3.5 x 10(-8) and 5.0 x 10(-8) M, respectively, at a signal-to-noise ratio of 3. Electrodes with laccase immobilized in Nation and... (More)
An amperometric enzyme electrode based on laccase for determining phenolic compounds is proposed. The following three types of polymer materials were used for enzyme immobilization on the surface of a glassy-carbon electrode: positively charged cetyl ethyl poly (ethyleneimine) (CEPEI) and negatively charged commercial Nafion and Eastman AQ 29D polymers. The advantages and disadvantages of each of the above polymers for enzyme immobilization are discussed. The detection limits of the model phenolic compounds hydroquinone and pyrocatechol in a buffer solution on laccase immobilization in a Nation membrane were 3.5 x 10(-8) and 5.0 x 10(-8) M, respectively, at a signal-to-noise ratio of 3. Electrodes with laccase immobilized in Nation and Eastman AQ 29D membranes exhibited the shortest response time. The operating stability and the stability in storage can be significantly improved by the additional incorporation of gelatin in the polymer matrices. Gelatin prevents enzyme inactivation as a result of enzyme modification by the free-radical oxidation products of phenolic compounds. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Analytical Chemistry
volume
60
issue
6
pages
553 - 557
publisher
MAIK Nauka/Interperiodica
external identifiers
  • wos:000229909800012
  • scopus:21544455541
ISSN
1608-3199
DOI
10.1007/s10809-005-0138-2
language
English
LU publication?
yes
id
578095a0-222b-416c-ac2e-2f4f451628b6 (old id 151005)
date added to LUP
2007-06-27 16:43:22
date last changed
2017-11-19 03:39:36
@article{578095a0-222b-416c-ac2e-2f4f451628b6,
  abstract     = {An amperometric enzyme electrode based on laccase for determining phenolic compounds is proposed. The following three types of polymer materials were used for enzyme immobilization on the surface of a glassy-carbon electrode: positively charged cetyl ethyl poly (ethyleneimine) (CEPEI) and negatively charged commercial Nafion and Eastman AQ 29D polymers. The advantages and disadvantages of each of the above polymers for enzyme immobilization are discussed. The detection limits of the model phenolic compounds hydroquinone and pyrocatechol in a buffer solution on laccase immobilization in a Nation membrane were 3.5 x 10(-8) and 5.0 x 10(-8) M, respectively, at a signal-to-noise ratio of 3. Electrodes with laccase immobilized in Nation and Eastman AQ 29D membranes exhibited the shortest response time. The operating stability and the stability in storage can be significantly improved by the additional incorporation of gelatin in the polymer matrices. Gelatin prevents enzyme inactivation as a result of enzyme modification by the free-radical oxidation products of phenolic compounds.},
  author       = {Yaropolov, A I and Shleev, Sergey and Morozova, O V and Zaitseva, E A and Marko-Varga, G and Emnéus, Jenny and Gorton, Lo},
  issn         = {1608-3199},
  language     = {eng},
  number       = {6},
  pages        = {553--557},
  publisher    = {MAIK Nauka/Interperiodica},
  series       = {Journal of Analytical Chemistry},
  title        = {An amperometric biosensor based on laccase immobilized in polymer matrices for determining phenolic compounds},
  url          = {http://dx.doi.org/10.1007/s10809-005-0138-2},
  volume       = {60},
  year         = {2005},
}